Six young tortoises Testudo marginata Schoepff, 1792 were experimentally infected with Hemolivia mauritanica (Sergent et Sergent, 1904). The prepatent period ranged from 6 to 8 weeks. Young, smaller, club-like forms (6-9 x 3-6 Am) of gametocytes appeared in the peripheral blood first, whereas mature, elongated, cylindrical forms (9-12 x 5-7 Am) were detected after 1-2 weeks and predominated during later patency. Three of the infected tortoises were euthanized and dissected to study the endogenous stages. Meronts occurred in the cells of the reticulo-endothelial system and in the erythrocytes; these were observed mostly in parenchymatous organs. Mature forms measured 14.2 x 9.3 microm and contained 7-12 merozoites. Cysts with two (exceptionally one) cystozoites were also found predominantly in parenchymatous organs and measured 14.8 x 7.9 microm. Pathological changes attributable to Hemolivia were mild and limited to liver and kidneys. The role of individual developmental stages of haemogregarines is discussed with respect to evolution of heteroxenous life cycle and long-term persistence of parasites in their intermediate hosts. 相似文献
ABSTRACT Sweet pepper-Leveillula taurica microclimate relations were studied under controlled conditions and in commercial greenhouses. Conidial germination occurred at 10 to 37 degrees C and was optimal at 20 degrees C. Conidial viability declined as temperatures increased to 40 degrees C for 6 h. Leaf colonization was optimal at 15 to 25 degrees C. Severe leaf infections occurred at 15 to 20 degrees C and conidiation was suppressed at 20 to 25 degrees C. Highest germination rates were observed at 75 to 85% relative humidity (RH). Severity of leaf coverage by symptoms was high for plants which were subjected to longer periods of temperatures between 10 to 15 degrees C and daytime RH between 85 to 95%, and positively correlated with nighttime RH. Disease severity was negatively correlated with lengthy periods of temperatures >25 degrees C, day and night average temperatures, and average daytime RH. Conversely, leaf shedding was relatively high under conditions characterized by long periods of temperatures >20 degrees C and <13 degrees C, and positively correlated with average daytime temperatures and periods of RH <75%. Increasing nighttime temperatures by heating and daytime temperatures by closing the greenhouse side walls reduced disease in two commercial greenhouse experiments. A midseason shift from a cooler greenhouse climate to warm daytime climate halted epidemic development. Flower number and yield were reduced in infected crops. 相似文献
ABSTRACT The basidiomycete Oncobasidium theobromae was identified as the cause of a devastating disease of cacao named vascular-streak dieback (VSD) in Papua New Guinea in the 1960s. VSD now causes losses among cacao seedlings and kills branches in mature cacao trees throughout Southeast Asia and parts of Melanesia. The characteristic symptoms include a green-spotted chlorosis and fall of leaves beginning on the second or third flush behind the stem apex, raised lenticels, and darkening of vascular traces at the leaf scars and infected xylem. Eventually complete defoliation occurs and, if the fungus spreads to the trunk, the tree will die. O. theobromae is a highly specialized, near-obligate parasite of cocoa. It is a windborne, leaf-penetrating, vascular pathogen, and may have evolved as an endophyte on an as yet unidentified indigenous host. The rate of disease spread on cocoa is limited because basidiocarps develop only on fresh leaf scars during wet weather, and basidiospores remain viable for a few hours on the night they are shed. Consequently, very few new infections occur beyond 80-m from diseased trees. Transmission of the disease through seed or infected cuttings has not been demonstrated. Strict quarantine measures applied to the movement of intact plants are crucial in reducing spread of the disease. Integrated management, including the planting of less susceptible genotypes, nursery construction and management, canopy pruning and shade management, provides effective control. 相似文献
Journal of Pest Science - Long-term monitoring networks that generate data on pest abundance are the foundation of integrated pest management. Monitoring can estimate local risk from pests and... 相似文献
Agroforestry Systems - Tropical agroforestry practices play a crucial role in mitigating global climate change by absorbing CO2 from the atmosphere through photosynthesis and storing carbon in... 相似文献
Phymastichus coffea LaSalle (Hymenoptera:Eulophidae) is an adult endoparasitoid of the coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera:Curculionidae:Scolytinae), which has been introduced in many coffee producing countries as a biological control agent. To determine the effectiveness of P. coffea against H. hampei and environmental safety for release in Hawaii, we investigated the host selection and parasitism response of adult females to 43 different species of Coleoptera, including 23 Scolytinae (six Hypothenemus species and 17 others), and four additional Curculionidae. Non-target testing included Hawaiian endemic, exotic and beneficial coleopteran species. Using a no-choice laboratory bioassay, we demonstrated that P. coffea was only able to parasitize the target host H. hampei and four other adventive species of Hypothenemus: H. obscurus, H. seriatus, H. birmanus and H. crudiae. Hypothenemus hampei had the highest parasitism rate and shortest parasitoid development time of the five parasitized Hypothenemus spp. Parasitism and parasitoid emergence decreased with decreasing phylogenetic relatedness of the Hypothenemus spp. to H. hampei, and the most distantly related species, H. eruditus, was not parasitized. These results suggest that the risk of harmful non-target impacts is low because there are no native species of Hypothenemus in Hawaii, and P. coffea could be safely introduced for classical biological control of H. hampei in Hawaii.
The source–sink ratio during grain filling is a critical factor that affects crop yield in wheat, and the main objective of this study was to determine the source–sink relations at both the canopy scale and the individual culm level under two nitrogen (N) levels at the post-jointing stage. Nine widely-used cultivars were chosen for analyzing source–sink relations in southwestern China; and three typical cultivars of different plant types were subjected to artificial manipulation of the grain-filling source–sink ratio to supplement crop growth measurements. A field experiment was conducted over two consecutive seasons under two N rates (N+, 150 kg ha–1; N–, 60 kg ha–1), and three manipulations were imposed after anthesis: control (Ct), removal of flag and penultimate leaves (Lr) and removal of spikelets on one side of each spike (Sr). The results showed that the single grain weights in the three cultivars were significantly decreased by Lr and increased by Sr, which demonstrated that wheat grain yield potential seems more source-limited than sink-limited during grain filling, but the source–sink balance was obviously changed by climatic variations and N deficient environments. Grain yield was highly associated with sink capacity (SICA), grain number, biomass, SPAD values, and leaf area index during grain filling, indicating a higher degree of source limitation with an increase in sink capacity. Therefore, source limitation should be taken into account by breeders when SICA is increased, especially under non-limiting conditions. Chuanmai 104, a half-compact type with a mid-sized spike and a long narrow upper leaf, showed relatively better performance in source–sink relations. Since this cultivar showed the characteristics of a lower reduction in grain weight after Lr, a larger increase after Sr, and a lower reduction in post-anthesis dry matter accumulation, then the greater current photosynthesis during grain filling contributed to the grain after source and sink manipulation. 相似文献
● Virtual joint centers on N agronomy were established between UK and China.● Key themes were improving NUE for fertilizers, utilizing livestock manures, and soil health.● Improved management practices and technologies were identified and assessed.● Fertilizer emissions and improved manure management are key targets for mitigation. Two virtual joint centers for nitrogen agronomy were established between the UK and China to facilitate collaborative research aimed at improving nitrogen use efficiency (NUE) in agricultural production systems and reducing losses of reactive N to the environment. Major focus areas were improving fertilizer NUE, use of livestock manures, soil health, and policy development and knowledge exchange. Improvements to fertilizer NUE included attention to application rate in the context of yield potential and economic considerations and the potential of improved practices including enhanced efficiency fertilizers, plastic film mulching and cropping design. Improved utilization of livestock manures requires knowledge of the available nutrient content, appropriate manure processing technologies and integrated nutrient management practices. Soil carbon, acidification and biodiversity were considered as important aspects of soil health. Both centers identified a range of potential actions that could be taken to improve N management, and the research conducted has highlighted the importance of developing a systems-level approach to assessing improvement in the overall efficiency of N management and avoiding unintended secondary effects from individual interventions. Within this context, the management of fertilizer emissions and livestock manure at the farm and regional scales appear to be particularly important targets for mitigation. 相似文献
Effects of adrenaline on the equilibrium distributions of Na+ , K+ , H+ , Cl– , and H2O across the cell membrane of rainbow trout (Salmo gairdneri) erythrocytes were determinedin vitro, as a function of P CO2 (1.76–7.77 torr). CO2-carrying capacity of the blood was also examined. Plasma catecholamine concentrations inunanaesthetized, unrestrained trout were 3.1 nM adrenaline and 1.2 nM noradrenaline. Elevation of the plasma adrenaline concentrationin vitro to 4.6 × 103 nM resulted in net gains of Na+ , Cl– and H2O by red cells, a net loss of H+ from red cells, and a pronounced red cell swelling. Adrenaline also reduced the CO2-carrying capacity of trout bloodin vitro. The magnitudes of these effects increased with PCO2 and, thus, were sensitive to blood HCO3– concentrations. The distribution of K+ between red cells and plasma was unaffected by adrenaline. Adrenergic-mediated ion movements and red cell swelling were sensitive to both propranolol and SITS. These results are consistent with the symport NaCl uptake model for adrenergic-mediated swelling of Baroinet al. (1984). The adrenergic response of fish erythrocytes may function to ameliorate the effects of blood acidoses on O2-carrying capacity by maintaining red cell pH in the face of a decrease in plasma pH. 相似文献