全文获取类型
收费全文 | 83篇 |
免费 | 11篇 |
专业分类
林业 | 4篇 |
基础科学 | 1篇 |
11篇 | |
综合类 | 18篇 |
农作物 | 3篇 |
水产渔业 | 2篇 |
畜牧兽医 | 34篇 |
园艺 | 13篇 |
植物保护 | 8篇 |
出版年
2023年 | 2篇 |
2022年 | 3篇 |
2021年 | 5篇 |
2020年 | 6篇 |
2019年 | 2篇 |
2018年 | 5篇 |
2017年 | 2篇 |
2016年 | 6篇 |
2015年 | 2篇 |
2014年 | 1篇 |
2013年 | 9篇 |
2012年 | 2篇 |
2011年 | 6篇 |
2010年 | 2篇 |
2009年 | 2篇 |
2008年 | 6篇 |
2007年 | 3篇 |
2006年 | 2篇 |
2005年 | 3篇 |
2004年 | 3篇 |
2003年 | 1篇 |
2002年 | 1篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1978年 | 2篇 |
1977年 | 1篇 |
1975年 | 1篇 |
1972年 | 1篇 |
1971年 | 1篇 |
1966年 | 1篇 |
1957年 | 1篇 |
1951年 | 1篇 |
1948年 | 1篇 |
1941年 | 1篇 |
排序方式: 共有94条查询结果,搜索用时 890 毫秒
81.
Fedoseeva Elena Stepanov Andrey Yakimenko Olga Patsaeva Svetlana Freidkin Mikhail Khundzhua Daria Terekhova Vera 《Journal of Soils and Sediments》2019,19(6):2688-2688
Journal of Soils and Sediments - Incorrect and incomplete wording: Funding information. The study was funded by the RFBR research project no. 18–016-00078. Correct and complete wording:... 相似文献
82.
The gene encoding equine herpesvirus 1 (equine abortion virus; EHV-1) glycoprotein D was engineered into the prokaryotic vector pEX, and expressed as a β-galactosidase fusion product, which was recognized by pooled equine sera and anti-EHV-1 rabbit sera. Antibodies raised against the EHV-1 gD fusion product identified strong bands in infected cells at 66 and 68 K and at 138 K in purified virus, thus characterizing the several forms of this major envelope glycoprotein which is an important candidate for inclusion in subunit vaccines. 相似文献
83.
Recent evidence suggests that feline members of the genus Porphyromonas are of consequence in periodontal disease in cats. Several possible virulence factors from feline strains of Porphyromonas gingivalis have been described that have similarities to those of human P. gingivalis. Both human and feline strains of P. gingivalis produce superoxide dismutase (SOD) which has been proposed as modulator of the inflammatory response during infection. The objective of this study was to clone the superoxide dismutase gene of feline P. gingivalis, to compare the characteristics of its product with that of the native enzyme and to determine its immunoreactivity in cats with periodontal disease. The sod gene of the feline strain Veterinary Pathology and Bacteriology (VPB) 3457 of P. gingivalis was amplified by PCR and cloned in frame with the alpha-peptide of the LacZ gene of E. coli in plasmid pUC19. This construct expressed SOD activity in E. coli with characteristics similar to those of the native SOD enzyme of P. gingivalis human strain 381 and the parent feline strain VPB 3457. The recombinant SOD had an apparent molecular weight of 54,700+/-1300 (S.E.M.) and was inactivated by 5mM hydrogen peroxide but not by 2mM KCN. There was a significant association (P=0.005) between the immunoreactivity of cats to P. gingivalis VPB 3457 soluble whole cell proteins on immunoblots and their responsiveness to the SOD protein. This suggests that cats showing a marked serum responsiveness to P. gingivalis itself, react to the SOD enzyme and further supports the role of feline P. gingivalis in periodontal disease. 相似文献
84.
Daria N. Love R.F. Jones Marilyn Bailey R.S. Johnson Nicola Gamble 《Veterinary microbiology》1982,7(5):455-461
Samples from 19 cases of feline empyaemia were examined. All cases had no prior treatment and specimens were collected by thoracocentesis after preparation of the skin as for aseptic surgery. Of the 87 bacterial strains isolated, 70 (80.5%) were anaerobes and 17 (19.5%) were facultative anaerobes. Fourteen cases contained mixtures of anaerobes and facultative anaerobes, and five contained anaerobes only. Bacteroides was the most commonly isolated genus (42.5% of all isolates). Clostridium villosum (16.1%) was the most frequently isolated bacterial species followed by Peptostreptococcus anaerobius and Pasteurella multocida (each 12.6%). The most commonly isolated anaerobic species was Clostridium villosum sp.nov. (20% of anaerobic isolates and 16.1% of all isolates) and Pasteurella multocida was the most commonly isolated facultatively anaerobic species (64.7% of facultative isolates and 12.6% of all isolates). 相似文献
85.
Michał Czopowicz Olga Szaluś‐Jordanow Marcin Mickiewicz Agata Moroz Lucjan Witkowski Iwona Markowska‐Daniel Daria Reczyńska Emilia Bagnicka Jarosław Kaba 《Animal Science Journal》2018,89(9):1364-1370
We carried out this study to determine for how long small ruminant lentivirus (SRLV)‐specific antibodies can be detected by three commercial ELISA kits in goat kids after suckling infected does in field conditions. Forty‐one kids born to SRLV‐seropositive asymptomatic does were blood sampled prior to colostrum consumption, and then weekly for 6 months in total. The sera were screened with three commercial ELISA kits: whole‐virus ELISA (wELISA), recombinant transmembrane and capsid antigen ELISA (TM/CA‐ELISA), and surface antigen ELISA (SU‐ELISA). All but one kid were seronegative in all three ELISAs right after birth. At the age of 1 week all kids turned seropositive in wELISA, 39 kids (95%) in TM/CA‐ELISA, and 35 kids (85%) in SU‐ELISA. All seropositive kids turned seronegative in wELISA by the 15th week, and in SU‐ELISA by the 19th week (median of 8 weeks in both ELISA), whereas in TM/CA‐ELISA five kids (13% of 39 initially seropositive) were still seropositive at the age of 6 months (median of 11 weeks). Antibody levels at the age of 1 week proved significantly linked to the duration of maternal antibodies in all three ELISAs and could be employed to predict for how long maternal antibodies would remain detectable. 相似文献
86.
Nematode eggs observed in cytology of cerebrospinal fluid diagnostic for intramedullary Spirocerca lupi spinal cord migration
下载免费PDF全文
![点击此处可从《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Sigal Klainbart Orit Chai Rafael Vaturi Kira Rapoport Itamar Aroch Merav Hagar Shamir 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2018,47(1):138-141
Spinal spirocercosis due to aberrant Spirocerca lupi nematode migration is an emerging etiology for acute myelitis in dogs in Israel, causing severe, mostly nonsymmetrical hind limb paresis or paralysis, and sometimes tetraparesis or tetraparalysis. So far, incidental identification of parasites during spinal surgery or at necropsy provides the only definite diagnosis, while antemortem diagnosis of this condition has been uncertain. Specifically, antemortem diagnosis is based on the typical clinical presentation of acute, progressive, asymmetrical hind limb paresis or paralysis, with moderate to severe eosinophilic to mixed cerebrospinal fluid (CSF) pleocytosis and increased CSF protein concentration. Exclusion of other differential diagnoses also requires using spinal cord imaging. In this novel report, we document a case of an intradural spinal spirocercosis in a dog, diagnosed antemortem, by detecting S lupi eggs in the CSF, and subsequent treatment, resulting in the resolution of the clinical signs. 相似文献
87.
Di Vincenzo D Maranz S Serraiocco A Vito R Wiesman Z Bianchi G 《Journal of agricultural and food chemistry》2005,53(19):7473-7479
The triacylglycerol, fatty acid, and polycyclic triterpene compositions of shea butter were determined for 150 samples from the sub-Saharan countries of Mali, Burkina Faso, Nigeria, and Uganda. The compositional profiles showed high variability in all three classes of compounds. Shea butter is made up mainly of four triglycerides (TAG) differing in carbon number (CN) by two, starting from CN 50 to CN 56. The greatest source of variation was in the CN 54 TAG. Shea butter is characterized by 16 saturated and unsaturated fatty acids in greatly varying proportion, the major ones being the even homologues in the range of C(16)-C(20). Oleic acid is dominant in Ugandan provenances, whereas stearic acid is dominant in West African shea butter. Acetyl and cinnamyl polycyclic triterpene means for countries ranged from 3.69 to 12.57%, with the highest values found in Nigerian provenances. Statistical comparisons of fat composition show that the geographic distance between shea populations is reflected in the degree of separation of their chemical profiles. 相似文献
88.
Daria A. Tur Nikita V. Khotskin Andrey E. Akulov 《Journal of animal physiology and animal nutrition》2021,105(5):984-988
This study aimed to assess the sex differences in the feeding behaviour of non-obese diabetic severe combined immunodeficient (NOD SCID) mice in a pharmacological model of type 1 diabetes mellitus (T1Dm). In our study, we chose NOD SCID mice of both sexes and assessed their feeding behaviour, body weight, body fat and water content under identical experimental conditions and diets. After 1 month of diabetes mellitus in mice in the experimental group, males and females did not show any increase in body weight, and they weighed significantly less than the control group. However, compared with the control group, in females with a background of T1Dm, there was a significant decrease in body fat. The amount of water consumed in the experimental groups was higher than that in the control groups. The amount of food consumed by males increased when they increased their water consumption, whereas food consumption in females decreased significantly with an increase in water consumption. Thus, we discovered sex differences in the feeding behaviour, body weight and body fat and water content in the pharmacological model of T1Dm after 1 month in NOD SCID mice. 相似文献
89.
The paper examines key factors affecting the marketing of various types of Non-Wood Forest Products and Services (NWFP&S)
through a comparative analysis of case studies in Europe, according to the main results of Working Group 3 (WG3) of the COST
Action E30 ‘Economic integration of urban consumers’ demand and rural forestry production. Seventeen Italian case studies
have been analysed in addition to the COST Action E30 experiences. Products and services are classified within a conceptual
framework developed by the WG3 and described in detail according to three main categories: mass-produced, specialized and
complementary NWFP&S. Especially this latter category can play a relevant role in improving the profitability of small and
medium-scale forest-based enterprises and in maintaining competitiveness of the forest product-consumer chain in rural areas,
but new marketing strategies are necessary for commercial success. Differentiation, integration and creation of networking
among both private and public actors and the development of the so-called ‘territorial marketing’ are considered the most
important tools for the role of complementary NWFP&S in improving the economic value of small-scale forestry in marginal areas. 相似文献
90.
Walsh T McClellan JM McCarthy SE Addington AM Pierce SB Cooper GM Nord AS Kusenda M Malhotra D Bhandari A Stray SM Rippey CF Roccanova P Makarov V Lakshmi B Findling RL Sikich L Stromberg T Merriman B Gogtay N Butler P Eckstrand K Noory L Gochman P Long R Chen Z Davis S Baker C Eichler EE Meltzer PS Nelson SF Singleton AB Lee MK Rapoport JL King MC Sebat J 《Science (New York, N.Y.)》2008,320(5875):539-543
Schizophrenia is a devastating neurodevelopmental disorder whose genetic influences remain elusive. We hypothesize that individually rare structural variants contribute to the illness. Microdeletions and microduplications >100 kilobases were identified by microarray comparative genomic hybridization of genomic DNA from 150 individuals with schizophrenia and 268 ancestry-matched controls. All variants were validated by high-resolution platforms. Novel deletions and duplications of genes were present in 5% of controls versus 15% of cases and 20% of young-onset cases, both highly significant differences. The association was independently replicated in patients with childhood-onset schizophrenia as compared with their parents. Mutations in cases disrupted genes disproportionately from signaling networks controlling neurodevelopment, including neuregulin and glutamate pathways. These results suggest that multiple, individually rare mutations altering genes in neurodevelopmental pathways contribute to schizophrenia. 相似文献