Investigation of genome polymorphism and seed coat anatomy of species of section Adenolinum from the genus Linum

Twelve species of section Adenolinum from genus Linum have been studied using cytogenetic methods (DAPI/C-banding, Ag-NOR staining, FISH with 5S and 26S rDNA probes), RAPD analysis, and seed surface ultrastructure and seed coat anatomy investigation. Karyotype analysis revealed that these species have a chromosome number of 2n = 18 or 2n = 36. The DAPI/C-banding patterns enabled the identification of all chromosomes and revealed the similarities in chromosome patterns in all of the studied species. It was found that 18-chromosome plants are diploids, whereas 36-chromosome forms are tetraploids. In diploid species, 5S and 26S rRNA genes are located in similar positions on a single pair of chromosomes or on two chromosome pairs in tetraploids. Ag-NOR staining showed NOR activity in both genomes of tetraploids. RAPD analysis and seed coat anatomy investigation revealed some interspecies differences. Species from nearby areas have more similar RAPD characteristics compared with more geographically distant species. The results of this study may serve as an additional argument in favour of a monophyletic origination of the Adenolinum species and its strong isolation from other Linum species.     

Tyzzer's disease in cats experimentally infected with feline leukaemia virus

Three cases of feline Tyzzer's disease have occurred, since 1971, in kittens infected experimentally with feline leukaemia virus (FeLV). It is suggested that the immunosuppression induced by FeLV may have predisposed the kittens to fatal infections with Bacillus piliformis.Clinical, pathological and ultrastructural features of the disease are described.     

A pathological study of the salivary glands of rabid dogs in the Philippines

Rabies is a zoonotic disease caused by the rabies virus. While the salivary glands are important as exit and propagation sites for the rabies virus, the mechanisms of rabies excretion remain unclear. Here, we investigated the histopathology of the salivary glands of rabid dogs and analyzed the mechanism of excretion into the oral cavity. Mandibular and parotid glands of 22 rabid dogs and three control dogs were used. Mild to moderate non-suppurative sialadenitis was observed in the mandibular glands of 19 of the 22 dogs, characterized by loss of acinar epithelium and infiltration by lymphoplasmacytic cells. Viral antigens were detected in the mucous acinar epithelium, ganglion neurons and myoepithelium. Acinar epithelium and lymphocytes were positive for anti-caspase-3 antibodies and TUNEL staining. In contrast, no notable findings were observed in the ductal epithelial cells and serous demilune. In the parotid gland, the acinar cells, myoepithelium and ductal epithelium all tested negative. These findings confirmed the path through which the rabies virus descends along the facial nerve after proliferation in the brain to reach the ganglion neurons of the mandibular gland, subsequently traveling to the acinar epithelium via the salivary gland myoepithelium. Furthermore, the observation that nerve endings passing through the myoepithelium were absent from the ductal system suggested that viral proliferation and cytotoxicity could not occur there, ensuring that secretions containing the virus are efficiently excreted into the oral cavity.     

Swallowtail butterflies show positive edge responses predicted by resource use

Context

The prevalence of edges is increasing due to anthropogenic landscape change. Edge responses can vary considerably between and within species. Understanding species’ responses to edges, and the causes of variation in such responses is central to managing biodiversity in contemporary landscapes.

Objective

A resource distribution model predicts that species that require complementary resources in different land cover types will be most abundant at edges, displaying a positive edge response. Eastern tiger (Papilio glaucus) and spicebush (P. troilus) swallowtail butterflies use forest plant species for oviposition sites but open-habitat plants for nectar. They are excellent models for testing the positive edge response and exploring sources of variability in edge responses, such as species-specific traits or temporal effects.

Methods

In southwestern Ontario, we examined both the abundance and flight orientation of these species in relation to forest/meadow edges and at different times of day. We used a transect method similar to the Pollard walk and a catch and release method, respectively.

Results

The distribution and flight behaviour of these butterfly species were overall consistent with a positive edge response. Both species were most abundant at the edge and oriented their flight towards the edge from the forest and meadow. However, P. glaucus demonstrated a much stronger positive edge response, while P. troilus showed temporal variation in its response.

Conclusions

Our results confirm the ability of the resource distribution model to predict species edge responses and movement behaviours, but also indicate that species-specific traits and time of sampling can influence such responses.

     

Immunogenicity of purified lipopolysaccharide or protein–oligosaccharide conjugates of Pasteurella multocida type 6:B in mice

Purified lipopolysaccharide (LPS) of Pasteurella multocida type 6:B, while toxic at higher doses, was protective at lower dose levels against experimentally-induced pasteurellosis in mice. However, the observed protection was abrogated if such LPS was digested with proteinase K prior to use in immunisation. The O-antigen polysaccharide side-chain (OS) of LPS did not appear to contribute to the observed protection as judged by the fact that immunisation of mice with purified OS or OS-protein conjugates, all of which were non-toxic, failed to confer protection against challenge with homologous virulent organisms. This was despite generation of significant levels of OS-specific antibodies, predominantly either of the IgM or IgG isotypes, in immunised mice.

Résumé

Le lipopolysaccharide purifié (LPS) de Pasteurella multocida type 6:B, toxique à haute dose, est protecteur à faible dose contre la pasteurellose expérimentale de la souris. Cependant, la protection observée est supprimée si le LPS est soumis à l'action de la protéinase K avant immunisation. L'antigène O polysaccharidique du LPS ne semble pas contribuer à la protection observée si l'on en juge par le fait que l'immunisation des souris à l'aide de l'antigène O purifé ou conjugué avec des protéines -- non toxiques -- ne confère pas de protection contre une épreuve virulente homologue. Ceci en dépit de l'apparition de taux significatif d'anticorps anti O principalement soit du type IgM ou IgG chez la souris.     

PATHOGENICITY OF A STRAIN OF FELINE CALICIVIRUS FOR DOMESTIC KITTENS

A strain of feline calicivirus, isolated from a cheetah exhibiting ulcerative glossitis and conjunctivitis, was administered by aerosol to 4 domestic cats and by contact to a fifth cat. Despite the limited number of animals available for the experiment, the pathogenicity of the virus strain for domestic cats was established. In aerosol-infected animals, clinical signs were referable to infection of both upper and lower respiratory tracts. The virus produced an interstitial pneumonia which, early in infection, was uncomplicated by secondary bronchopneumonia. The in-contact cat exhibited clinical signs referable to infection of the oral cavity only and no lesions were noted in the lower respiratory tract at autopsy. Ulcerative glossitis was a feature of the disease in aerosol-infected and in-contact cats. The virus was isolated from the pharynx of all cats for up to 21 days after infection and from the tonsils at autopsy. The tonsils were considered to be a probable site of multiplication of virus in "carrier" cats.     

Bacteria use type IV pili to walk upright and detach from surfaces

Bacterial biofilms are structured multicellular communities involved in a broad range of infections. Knowing how free-swimming bacteria adapt their motility mechanisms near surfaces is crucial for understanding the transition between planktonic and biofilm phenotypes. By translating microscopy movies into searchable databases of bacterial behavior, we identified fundamental type IV pili-driven mechanisms for Pseudomonas aeruginosa surface motility involved in distinct foraging strategies. Bacteria stood upright and "walked" with trajectories optimized for two-dimensional surface exploration. Vertical orientation facilitated surface detachment and could influence biofilm morphology.     

Characterization and Biocompatibility Properties In Vitro of Gel Beads Based on the Pectin and κ-Carrageenan

This study aimed to investigate the influence of kappa (κ)-carrageenan on the initial stages of the foreign body response against pectin gel. Pectin-carrageenan (P-Car) gel beads were prepared from the apple pectin and κ-carrageenan using gelling with calcium ions. The inclusion of 0.5% κ-carrageenan (Car0.5) in the 1.5 (P1.5) and 2% pectin (P2) gel formulations decreased the gel strength by 2.5 times. Car0.5 was found to increase the swelling of P2 gel beads in the cell culture medium. P2 gel beads adsorbed 30–42 mg/g of bovine serum albumin (BSA) depending on pH. P2-Car0.2, P2-Car0.5, and P1.5-Car0.5 beads reduced BSA adsorption by 3.1, 5.2, and 4.0 times compared to P2 beads, respectively, at pH 7. The P1.5-Car0.5 beads activated complement and induced the haemolysis less than gel beads of pure pectin. Moreover, P1.5-Car0.5 gel beads allowed less adhesion of mouse peritoneal macrophages, TNF-α production, and NF-κB activation than the pure pectin gel beads. There were no differences in TLR4 and ICAM-1 levels in macrophages treated with P and P-Car gel beads. P2-Car0.5 hydrogel demonstrated lower adhesion to serous membrane than P2 hydrogel. Thus, the data obtained indicate that the inclusion of κ-carrageenan in the apple pectin gel improves its biocompatibility.     

Migratory passerine birds in Britain carry Phytophthora ramorum inoculum on their feathers and “feet” at low frequency

In this study, we investigated whether birds could be vectors facilitating long‐distance spread of Phytophthora ramorum in Britain. Migratory bird species associated with the main sporangium‐producing host plants and most likely to pick up P. ramorum spores were considered. Swabs were taken from the flank and “feet” of 1,014 birds over a 12‐month period (April 2011–March 2012) in the west of Britain and subsequently analyzed for the presence of P. ramorum using nested PCR. Ten positive samples from 10 birds were identified: three in Cornwall, one in Devon, three in Gloucestershire, two in north Wales and one in Merseyside. Phytophthora ramorum was detected on samples from four species of thrushes (Redwing Turdus iliacus, Fieldfare T. pilaris, Blackbird T. merula and Song Thrush T. philomelos) and one species of warbler (Chiffchaff Phylloscopus collybita). All birds that tested positive were sampled in late autumn and winter (October–February), when long‐distance movements (over 100 km) would have stopped. The low incidence of P. ramorum found using PCR suggests that the incidence of inoculum, whether viable or not, on birds was low. The apparently low incidence of inoculum on birds suggests migratory passerine birds can carry P. ramorum inoculum on their feathers and “feet,” albeit at low frequency. The dates of positive samples indicate that birds would not have been moving long distances at the time but further work is needed to estimate the extent of their contribution to the spread of P. ramorum in Britain.