Enzyme activities and microbial community structure in semiarid agricultural soils

This study investigated the effect of management on -glucosidase, -glucosaminidase, alkaline phosphatase, and arylsulfatase activities and the microbial community structure in semiarid soils from West Texas, USA. Surface samples (0–5 cm) were taken from a fine sandy loam, sandy clay loam, and loam that were under continuous cotton ( Gossypium hirsutum L.) or in cotton rotated with peanut ( Arachis hypogaea L.), sorghum ( Sorghum bicolor L.), rye ( Secale cereale) or wheat ( Triticum aestivum L.), and had different water management (irrigated or dryland), and tillage (conservation or conventional). The enzyme activities were higher in the loam and sandy clay loam than in the fine sandy loam. Soil pH was not affected by management, but the soil organic C and total N contents were generally affected by the different crop rotations and tillage practices studied. The trends of the enzyme activities as affected by management depended on the soil, but in general crop rotations and conservation tillage increased the enzyme activities in comparison to continuous cotton and conventional tillage. The soil enzyme activities were significantly correlated with the soil organic C ( r -values up to 0.90, P< 0.001), and were correlated among each other ( r -values up to 0.90, P <0.001). There were differences in the fatty acid methyl ester profiles between the fine sandy loam and the sandy clay loam and loam, and they reflected the differences in the enzyme activities found among the soils. For example, a 15:0 ranged from 1.61±0.25% in cotton-peanut/irrigated/no-till in the fine sandy loam to 3.86±0.48% in cotton-sorghum/dryland/conservation tillage in the sandy clay loam. There were no differences due to management within the same soil.Trade names and company names are included for the benefit of the reader and do not infer any endorsement or preferential treatment of the product by USDA-ARS     

The environments of loess uplands to the north and east of the Carpathians during the penultimate interglacial (MOIS 7) in palaeopedological and palaeobotanical records

The stratigraphic rank of warming, distinguished in marine deposits as ‘marine oxygen isotope stage 7’ (MOIS 7) and recorded in terrestrial deposits as a palaeosol, has been discussed for a long time. The purpose of this paper is to describe the climate and environment of the time, determined from palaeopedological and palynological investigations of loess‐palaeosol sequences in the peri‐ and metha‐Carpathian zones, and to correlate these sequences with the Pleistocene stratigraphic units. The changes in chemical composition of the deposits were used to reconstruct the processes of chemical weathering and translocation of mobile elements during pedogenesis in three sections (Orzechowce, Galych and Velykyi Glybochok). The variability of the mean annual temperature and precipitation was estimated from the changes of elemental ratios resulting from chemical weathering and vegetation composition. From micromorphological analysis, the stages of palaeosol development and their transformation in cold periods were reconstructed. Pollen analysis carried out for the Velykyi Glyboczok, Maksymivka and Stari Kodaky sites, was used to reconstruct the vegetation cover and the temperature changes of the coldest and warmest months were calculated. The results indicate that the penultimate interglacial period, correlated with MOIS 7, comprised two warming periods separated by a profound cooling period. Warm periods were cooler than the last interglacial and more recent conditions. Vegetation was characterized by the co‐occurrence in changing proportions of patches of mixed forests and steppe vegetation. In the cold period, permafrost developed in places and cryogenic structures were formed. Regional differentiation of biotope environments, resulting from the increase in the continental nature of the climate towards the east, occurred during the whole interglacial period.     

IMPACT OF SELENIUM FERTILIZATION ON GLUCOSINOLATE CONCENTRATION IN ARABIDOPSIS THALIANA AND RAPID CYCLING BRASSICA OLERACEA

Brassica vegetables are a significant source of glucosinolates (GSs), which are sulfur (S)-containing phytonutrients exhibiting, upon hydrolysis by endogenous myrosinase, antioxidant and anti-carcinogenic activity. Selenium (Se) is an essential micronutrient in mammalian health exhibiting antioxidant activity by inhibiting experimental carcinogenesis in animal models and reducing cancer incidence in human clinical trials. Selenium is readily accumulated in both Brassica species and Arabidopsis thaliana, a model species with similar S-metabolism. The research objectives for this project were to: 1) compare the impact of Se fertilization on GS concentrations between Arabidopsis thaliana and rapid cycling Brassica oleracea; and 2) determine the level of Se fertilization needed to optimize plant tissue Se concentration to maximize potential human health benefits. Both S and Se concentrations increased in A. thaliana and B. oleracea tissues in response to increasing Se treatments. Concentrations of glucoiberin, glucoraphanin, aliphatic, and total GS differed significantly between A. thaliana and B. oleracea. Data demonstrates that anti-carcinogenic GSs can be modified through changes in Se treatment concentrations, yet high levels of anti-carcinogenic GSs can be maintained while increasing Se concentration to 0.8 mg Se L^-1. Thus, it is feasible to increase Se to beneficial dietary levels without compromising GS concentrations.     

Evaluation of Pine Bark or Pine Bark with Goat Manure or Sewage Sludge Cocomposts As Growing Media for Vegetable Seedlings

The suitability as growing media of composts made from pine bark or pine bark cocomposted with goat manure or sewage sludge and either inoculated or not inoculated with effective microorganisms, was evaluated under greenhouse conditions with and without fertilization using cabbage as the test crop. The treatments were replicated three times and arranged in a randomized complete block design in a fibre glass covered greenhouse. Cabbage seedlings were grown in cavity trays for five weeks, after which plants were harvested and fresh and dry weights determined. Samples were also analyzed for N, P, K and selected heavy metal concentrations. Results revealed that pine bark-goat manure cocomposts supported good seedling growth and could thus be good substitutes for pine bark alone as a growing medium where goat manure or similar manures are available. The results also showed that despite the superior nutritional value of these alternative growing media, nutrient supplementation may still be necessary where seedlings are kept in the nursery for extended periods due to nutrient exhaustion through plant uptake and leaching. Pine bark-sewage sludge compost also had positive effects on seedling growth but could only be recommended as a growing medium for nonfood plants because its composting did not reach the thermophillic temperatures necessary for adequate pathogen kill. Inoculation with effective microorganisms improved seedling growth in sewage sludge and goat manure based composts but the mechanisms involved remain to be established.     

Influence of temperature,humidity duration and growth stage on the infection and mycotoxin production by Fusarium langsethiae and Fusarium poae in oats

High occurrence of Fusarium poae (FP) and Fusarium langsethiae (FL) and their mycotoxins nivalenol (NIV) and T-2/HT-2 have been observed in Swiss oats. Early prediction of mycotoxin levels is important for farmers and the cereal industry to minimize the risk of contaminated food and feed. Therefore, climate chamber experiments were conducted to investigate the influence of different temperatures (10, 15, 20 °C) and durations (4, 8, 12 h) at 99% relative humidity (RH) on the infection of oats with FP and FL. In addition, to discover the most susceptible period of oats, artificial FL inoculations were conducted at different growth stages. Field experiments were performed to observe the dispersal of these fungal species within the field and to investigate the weather conditions that influence the dispersal. The climate chamber experiments revealed higher contamination with NIV and T-2/HT-2 in the 10 °C treatments and with a prolonged humidity duration of 12 h 99% RH. Inoculations of oat plants at early (DC 61) and mid (DC 65) anthesis, led to higher FL infection and T-2/HT-2 accumulation in the grains compared with treatments at earlier growth stages, which might be due to an increased susceptibility during anthesis. No indication for spore dispersal was observed in the field experiments. The results obtained, together with the cropping factors that influence infection and mycotoxin production, could be used as a first step in developing forecasting models to predict the contamination of oats with the mycotoxins NIV and T-2/HT-2.     

Aerosol transmission of arthropathic and amyloidogenic Enterococcus faecalis.

One-day-old brown layer chicks were exposed to an aerosol of an arthropathic and amyloidogenic Enterococcus faecalis strain alone or after being subjected to treatment with formaldehyde gas (100-200 ppm). Four-day-old chicks were also treated with the same aerosol but after treatment with a Newcastle disease vaccine virus (NDVV) aerosol or intramuscular injection with methylprednisolon at day 1. The same E. faecalis strain was inoculated intramuscularly in day-old chicks as positive control. Bacteremia with time showed that 24 hr after the aerosol the day-old exposed chicks had the highest rate of positive blood cultures (70%-80%). Lower numbers of bacteremic birds at this point in time were found in the chicks treated with E. faecalis aerosol at day 4 (3/10 in the methylprednisolon-treated group and 0/10 in the NDVV-treated group) and the E. faecalis intramuscular-injected group at day 1 (2/10). Formaldehyde gas treatment did not favor the occurrence of bacteremia. NDVV aerosol exposure or injection with corticosteroids did not favor the occurrence of bacteremia 24 hr after E. faecalis aerosol exposure at day 4 either, although 66 days after aerosol, one bird (1/14) treated with NDVV showed bacteremia. A few bacteremic birds were found 10 days after aerosol in the NDVV- and methylprednisolon-treated groups, whereas at 14 days after aerosol, one bacteremic bird was seen in the group subjected to E. faecalis aerosol at day 1, indicating the occurrence of chronic bacteremia. In contrast to the E. faecalis intramuscular-inoculated birds, no joint pathology was seen in the aerosol-exposed groups in spite of the occurrence of chronic bacteremia.     

Comparison of metrizamide and iohexol for cisternal myelographic examination of dogs

A double-blind study, using metrizamide, iohexol, or Ringer's solution (control) as cisternal myelographic agents, was performed on 25 dogs. Before myelographic examination was done, each dog was subjected to physical, clinical pathologic, and neurologic examinations, as well as examinations by electroencephalography and computerized tomography. These were repeated 24 hours after completion of the myelographic examination. The group of dogs given metrizamide (group II) had a significantly greater occurrence of seizure activity (6 of 10) than did the control dogs (group I; 0 of 5) or dogs given iohexol (group III; 0 of 10; P less than 0.003). In group II, the CSF microprotein concentration was significantly greater 24 hours after myelography was done than were the values in groups I and III (P less than 0.003). Myelograms of the group II dogs (metrizamide) and group III dogs (iohexol) had similar diagnostic qualities. At 24 hours after myelographic examination was done, computerized tomography scan revealed that each dog given metrizamide and iohexol had myelographic contrast material in the brain and cervical spinal cord parenchyma. Seemingly, iohexol has good diagnostic quality, but is less epileptogenic than metrizamide when used in cervical myelographic examinations of dogs.     

Milk vetch dwarf virus infection in the Solanaceae and Caricaceae families in Southeast Asia

Milk vetch dwarf virus (MDV) is an important member of the genus Nanovirus and is transmitted by the aphid Aphis craccivora. MDV has multiple single-stranded DNA genome components, each approximately 1 kb, and two or three alpha-satellite molecules. It mainly infects plants of the legume family Fabaceae. Recently, papaya (Carica papaya) collected in Yesan, South Korea, displaying symptoms of leaf yellowing and dwarfism, was identified as a new host for MDV. To examine the geographical distribution of MDV, papaya samples with symptoms were harvested in South Korea, Vietnam, and Taiwan in August 2018, along with tomato and pepper samples grown in adjacent fields in Vietnam. The results revealed the presence of MDV not only in papaya but also in pepper and tomato. This MDV infection in members of the Solanaceae family was confirmed by Southern blot hybridization performed using a PCR product of segment S as a probe. Based on sequence analysis of three MDV components (M, S, and C3), we verified the presence of three different isolates of MDV in these three countries and homology between sequences of isolates from papaya and from members of the Solanaceae from Vietnam. Taken together, our results clearly demonstrate MDV infection in Vietnam and Taiwan for the first time and confirm that MDV can infect economically important pepper and tomato.