虾蛄蛋白浆对河蟹摄食和生长影响的研究

分别用虾蛄蛋白浆替代饲料中2%和4%的鱼粉,并以乌贼膏为对照,研究其对河蟹摄食和生长的影响。结果表明,饲料中添加虾蛄蛋白浆对河蟹的诱食作用与乌贼膏相当,但摄食量显著高于乌贼膏组。此外,虾蛄蛋白浆还具有明显的促进河蟹脱壳生长的作用,其作用机制可能与其中氨基酸、小肽、胆固醇等含量丰富有关。     

22种植物提取液对杨分月扇舟蛾的生物活性的影响

对22种植物提取液的杀虫活性进行了初步筛选,结果表明苍耳植物提取液对杨分月扇舟蛾2龄幼虫有很强的拒食作用,其种子提取液对供饲叶碟24 h和48 h后的拒食率分别为82.83%和75.15%,茎提取液24 h和48 h后的拒食率分别为55.56%和65.87%。苍耳种子、地锦全株和牛膝叶提取液对杨分月扇舟蛾幼虫有非常强的毒杀作用,6 d后校正死亡率分别为91.94%、83.87%和81.13%。     

Study on the Tissue Culture of Coleus blumei

This paper studied on the way of Coleus blumei, the leaf was chosen as explants and was inoculated on 1/2 MS medium with different combinations of 6-benzyladenine （6-BA）, α-naphthaleneacetic acid （NAA） and indole-3-butyric acid （IBA）. The optimal conditions of callus induction from explants were achieved on the medium containing 6-BA （2.0 mg· L^-1） and NAA （1.0 mg· L^-1）. Shoot tips were induced on the medium containing 6-BA （4.0 mg·L^-1） and NAA （0.5 mg·L^-1）. The same media conditions were found suitable for shoot multiplication, we multiplied shoots rooted best on 1/2 MS medium supplemented with IBA （0.1 mg·L^-1）.     

犬新孢子虫NcSRS2基因原核表达质粒的构建

根据已发表的犬新孢子虫NcSRS2基因序列,设计1对含有EcoRⅠ和NotⅠ酶切位点的引物。以提取犬新孢子虫虫体基因组DNA为模板,应用PCR扩增获得NcSRS2 ORF基因片段,将此基因片段克隆到pMD18-T Simple载体上,用EcoRⅠ和NotⅠ双酶切该片段,回收得到含有两个酶切位点黏端的Nc-SRS2 ORF基因,将此基因片段克隆至相同酶切回收后的PGEM-4T-2原核表达载体中,获得重组质粒pGEX-NcSRS2,经PCR鉴定,限制性内切酶分析和克隆片段序列测定比较,证实了重组质粒的正确性。     

海岸带风口沙地提高木麻黄造林效果的研究

自１９９８年５月以来,在福建省惠安县赤湖林场和东山县赤山林场木麻黄基干林带前沿风口干旱沙地,进行提高木麻黄造要效果配套技术研究。经２年试验研究,在分析风口困难立地造林失败主要原因基础上,从木麻黄抗逆品系选择应用,因地制宜确定造林方式,采用深挖整地、放客土、拌泥浆、提早造林季节、选择大雨天冒雨造林、大苗深栽等抗旱造林配套技术以及旱季培土抚育保墒、浇水保苗和筑沙堤设风障防潮防风工程措施相结合的造林方法     

Isolation and Characterization of GhLEA3 Gene from Upland Cotton and Its Expression in Response to Low Temperature Stress

[Objective] Based on the analysis of cotton GhLEA3 gene structure and its expression model in chilling stress, we investigated the resistance function of GhLEA3 in response to cold stress. [Method] We cloned GhLEA3 from upland cotton by homologous sequence cloning way. We analyzed the properties of the GhLEA3, and constructed phylogenetic tree by bioinformatics analysis. We constructed the transient expression vector 35S∷GhLEA3-GFP by In-Fusion connection technology and studied the subcellular localization of GhLEA3. The expression of GhLEA3 gene in leaves of TM-1 at three-leaf stage under low temperature stress treatments (4 ℃, 24 h) was performed. Agrobacterium tumefaciens mediated floral dipping method was used to transform the gene with expression vector 35S∷GhLEA3-GFP into Arabidopsis thaliana wild type. Low temperature germination experiment at 4 ℃ was conducted to verify germination ability of the transgenic Arabidopsis thaliana T₃ seed. The transgenic Arabidopsis seedlings were treated at 4 ℃(low temperature), and all the leaves were taken to measure their electrical conductivity. [Result] The coding sequence of GhLEA3 gene is 1 218 bp, which encodes 405 amino acids. GhLEA3 protein includes 4 functional domains of PF02987. Phylogenetic analysis showed that the similarity of amino acid sequences between upland cotton GhLEA3 and Arabidopsis thaliana AtLEA3 was 52.6%. GhLEA3 might be mainly localized in vacuoles and small vesicles. GhLEA3 was up-regulated in leaves after low temperature treatment. The germination rate of transgenic Arabidopsis thaliana of T₃ generation at low temperature was significantly higher than that of wild type, and its electrical conductivity of leaves after low temperature treatment was significantly lower than that of wild type. [Conclusion] GhLEA3 belonged to the member of LEA3 family. Phylogenetic analysis showed that GhLEA3 had the closest relationship with AtLEA3. GhLEA3 was induced by low temperature stress and may play an important role in improving cold resistance.     

化肥减施对烤烟根系和烟株产质量的影响

通过减施化肥增施不同种类和数量有机肥对烤烟根系和产质量的影响的研究,结果表明:(1)减施化肥增施有机肥提高了烟株根系活力,以有机肥施用量较多的处理表现较好,相同有机肥施用量比较又以施用生物有机肥的处理较好些。(2)减施化肥增施有机肥降低了根系鲜干重和田间病害发病率,但随着有机肥施用量的增加,根系鲜干重有逐渐增加的趋势,田间病害发病率有降低的趋势。(3)上等烟比例、均价和产值均以施常规化肥750 kg.hm-2+精致有机肥225 kg.hm-2处理较高。减施化肥增施有机肥处理相比,随着减施化肥和增施有机肥数量的增加,上等烟比例和均价逐渐增加,但产量和产值下降。(4)减施化肥增施有机肥提高了钾含量,降低了烟碱、总糖、还原糖、淀粉和氯含量,但各处理相差不大。(5)减施化肥增施有机肥各处理烤后烟叶评吸质量差异较小,基本属同一档次,对感官评吸的影响主要在杂气和刺激性方面有所差异。     

利用RAPD引物对四川乳肉兼用牛及其父母本的遗传距离鉴定

为了鉴定四川乳肉兼用牛与父母本的亲缘关系,从50条随机引物中筛选出6条引物对四川乳肉兼用牛及其父母本进行扩增.6条RAPD引物共扩增出334个条带.将这些多态性条带进行聚类分析,结果显示:四川乳肉兼用牛与3个父母本品种牛的遗传差距并不大,在0.01～0.25之间,其中与西门塔尔牛遗传距离最近,与宣汉本地黄牛的遗传距离最远.这与传统的形态学结果一致.     

不同饲养方式对肉鸡肌纤维组织学特性及肌肉嫩度影响的研究

[目的]研究了不同饲养方式对河田鸡肌纤维组织学特性及肌肉嫩度的影响,为肉鸡品质调控提供理论依据。[方法]研究户外散养、室内平养、室内笼养3种饲养方式对不同日龄河田鸡腿肌和胸肌肌纤维组织学特性的影响及其与肌肉嫩度的关系。[结果]结果表明,户外散养鸡的胸肌和腿肌肌纤维直径和肌肉剪切力显著大于笼养鸡（P〈0．05）,而肌纤维密度显著低于笼养鸡（P〈0．05）。随着日龄的增加,肌纤维的直径和肌肉剪切力显著增加,而肌纤维密度显著下降。公鸡的肌纤维直径和剪切力大于母鸡。相关性分析表明,胸肌肌肉剪切力与肌纤维直径呈显著正相关（P〈0．05）,而与肌纤维密度呈负相关（P〈0．05）;腿肌肌肉剪切力与肌纤维直径和密度关系不显著（P〉0．05）。[结论]饲养方式对肉鸡肌纤维的组织学特性有显著影响,户外散养促进肉鸡肌纤维发育,相应增加了肌肉剪切力。     

弥河流域重金属在冬小麦不同器官中的富集特征

农作物中的重金属含量是影响其品质与安全的重要方面。为了探讨山东省弥河流域不同条件下重金属在冬小麦根系-茎叶-籽粒中的富集特征,该文采用实地调查和室内分析相结合的方法进行了研究,用电感偶合等离子光谱仪（ICP-AES）分别测定了研究区所采集样点的土壤和植物样品中的重金属Cr、Cu、Ni、Zn、Mn和V的全量,用数理统计法进行了分析。结果表明：冬小麦对重金属Cr、Cu、Ni、Zn、Mn和V的富集能力差异明显,根系对重金属富集能力由强到弱的顺序是：Zn〉Cu〉Ni〉Mn〉Cr〉V,茎叶对重金属富集能力由强到弱的顺序是：Zn〉Cu〉Mn〉Cr〉Ni〉V,籽粒对重金属富集能力由强到弱的顺序是：Zn〉Cu〉Mn〉Ni〉Cr〉V;冬小麦不同器官对重金属的富集能力不同,Cr、和Mn富集能力的顺序是：根系〉茎叶〉籽粒;重金属Cu、Ni、和Zn富集能力的顺序是：根系〉籽粒〉茎叶;而重金属V只有在根系富集,茎叶和籽粒均不富集重金属V。