除草剂最低致死剂量(MLHD)使用新技术概述

本文论述了荷兰MLHD技术的基本内容、技术原理、应用程序等．并针对荷兰的农业特点、开发背景，对该技术在荷兰与中国的应用前景及可行性等进行了比较分析．作者认为合理地利用该技术将有利于我国农业的可持续发展。     

Taxonomic and Bioactivity Characterizations of Mameliella alba Strain LZ-28 Isolated from Highly Toxic Marine Dinoflagellate Alexandrium catenella LZT09

Microalgae host varied microbial consortium harboring cross-kingdom interactions with fundamental ecological significance in aquatic ecosystems. Revealing the complex biofunctions of the cultivable bacteria of phycosphere microbiota is one vital basis for deeply understanding the mechanisms governing these dynamic associations. In this study, a new light-yellow pigmented bacterial strain LZ-28 was isolated from the highly-toxic and harmful algal bloom-forming dinoflagellate Alexandrium catenella LZT09. Collective phenotypic and genotypic profiles were obtained to confidently identify this strain as a new Mameliella alba member. Comparative genomic analysis showed that strain LZ-28 shared highly similar functional features with other four marine algae-derived M. alba strains in spite of their distinctive isolation sources. Based on the bioactivity assaying, the mutual growth-promoting effects between bacterial strain LZ-28 and algal strain LZT09 were observed. After the culture conditions were optimized, strain LZ-28 demonstrated an extraordinary production ability for its bioflocculanting exopolysaccharides (EPS). Moreover, the portions of two monosaccharides glucose and fucose of the EPS were found to positively contribute to the bioflocculanting capacity. Therefore, the present study sheds light on the similar genomic features among the selected M. alba strains, and it also reveals the potential pharmaceutical, environmental and biotechnological implications of active EPS produced by this new Mameliella alba strain LZ-28 recovered from toxic bloom-forming marine dinoflagellate.     

美洲鲥鱼嗜水气单胞菌病原的分离与鉴定

2021年,山东省临沂市一养殖场养殖的美洲鲥鱼(Alosa sapidissima)突发疾病并出现严重死亡,日死亡率高峰期达到2.5%,累积死亡率约为90%。患病鱼主要症状为体表出血、溃疡,解剖可见腹腔腹水、肝脏暗红,并伴有肠炎。组织病理学检测发现,病鱼肝脏出现弥散性坏死,嗜碱性粒细胞增多和细胞肿胀空泡变性;脾脏出现出血性贫血性坏死灶、核破裂和核固缩;肾脏淋巴细胞坏死脱落,肾小体毛细血管球萎缩,近端小管和远端小管内的细胞出现不同程度的细胞结构消失。发病鱼肉眼和显微镜观察未见明显寄生虫,利用PCR方法检测鲤疱疹病毒2型(Cyprinid herpesvirus 2)、鲈鱼蛙病毒(largemouth bass ranavirus)等淡水鱼类常见病毒均为阴性。细菌分离培养结果显示,从发病鱼的肝脏、肾脏和脾脏中分离得到形态一致的优势菌,命名为AS-AH2101。经16S rRNA测序比对和生理生化鉴定,确定AS-AH2101为嗜水气单胞菌(Aeromonas hydrophila)。毒力基因检测结果显示,AS-AH2101携带气溶素(aerA)、溶血素(hlyA)、丝氨酸蛋白酶(ahpA)、...     

半轴套管热挤压成形工艺数值模拟

通过对半轴套管的工艺分析,基于体积不变原则,采用逆向法计算零件毛坯,确定了热挤压成形工艺方案;结合刚粘塑性有限元法基本原理,运用有限元软件对半轴套管的反挤压过程进行3-D热力耦合模拟。影响半轴套管热挤压成形的主要工艺参数有挤压温度、挤压速度、模具预热温度等。在其他条件相同情况下,分别设置不同的挤压温度、挤压速度、模具预热温度,通过对不同参数模拟结果的分析比较,得出工艺参数对半轴套管成形的影响状况,揭示热挤压成形过程中的金属流动规律。优化的成形参数,提高了半轴套管的成形性能和经济性能。     

桐梓县杂交玉米氮磷钾的合理配施效应

采用"3414"平衡施肥方案,在桐梓县肥力较高的小黄沙泥土上进行了杂交玉米的肥料效应田间试验.结果表明,增产效果最佳的肥料配方为处理6,即尿素32.6kg·667m-2、普钙62.5kg·667m-2、氯化钾26.7kg·667m-2;从经济效益分析来看,以低氮低磷中钾处理的肥料增产效益最好,其肥料的产/投比为2.42,其肥料配方为处理12(N1P1K2),即尿素13.6kg·667m-2、普钙31.25kg·667m-2、氯化钾13.3kg·667-2.     

浅谈呼玛县林业局森林资源调查与发展规划

文章根据呼玛县林业局森林资源现状及发展规划,提出几点经营措施供参考。     

江苏省铜山县日光温室现状及选型问题的探讨

日光节能温室是我县20世纪90年代迅速发展起来的一种蔬菜生产的保护地形式,与过去的玻璃温室相比,具有结构简单、造价低、使用方便、节能、经济效益高等特点.但是,温室结构、工程造价、配套技术等方面基本上没有统一和规范,存在着很大的随意性,影响标准化配套技术的普及推广,影响着温室结构优化.本文即对我县蔬菜日光温室的现状进行了较深入的调查,对高效节能型温室的选型、结构参数提出了一些改进措施.     

Oncolytic Vaccinia Virus Carrying Aphrocallistes vastus Lectin (oncoVV-AVL) Enhances Inflammatory Response in Hepatocellular Carcinoma Cells

Aphrocallistes vastus lectin (AVL) is a C-type marine lectin derived from sponges. Our previous study demonstrated that oncolytic vaccinia virus carrying AVL (oncoVV-AVL) significantly enhanced the cytotoxicity of oncoVV in cervical cancer, colorectal cancer and hepatocellular carcinoma through the activation of Ras/ERK, MAPK/ERK and PI3K/Akt signaling pathways. In this study, the inflammatory response induced by oncoVV-AVL in a hepatocellular carcinoma cell (HCC) model was investigated. The results showed that oncoVV-AVL increased the levels of inflammatory cytokines including IL-6, IL-8 and TNF-α through activating the AP-1 signaling pathway in HCC. This study provides novel insights into the utilization of lectin AVL in the field of cancer therapy.     

当前我国农村土地制度改革的必要性与对策思考

我国农村土地制度与"三农"问题紧密相关。与其他国家的农村土地制度相比,我国的农村土地制度更具独特性,因此推进我国农村土地制度改革必须立足基本国情,而不能照抄他国的土地制度。该文分析了当前我国农村土地制度的必要性,并对推进我国农村土地制度改革提出对策思考。     

影响我国水产饲料产业升级的科学性和技术性问题分析

我国的水产饲料工业发展历程非常短,真正开展水产动物营养研究与饲料生产应该是在20世纪80年代后期了,到现在不过30年的发展时间,但水产饲料工业的发展速度是非常快的,到2008年全国饲料总量为13 700万吨(2008中国饲料工业年鉴),而水产饲料为1 342万吨、占全国饲料总量的9.79%.