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941.
Fusarium graminearum TRI14 is required for high virulence and DON production on wheat but not for DON synthesis in vitro 总被引:1,自引:0,他引:1
Dyer RB Plattner RD Kendra DF Brown DW 《Journal of agricultural and food chemistry》2005,53(23):9281-9287
Fusarium head blight (FHB) of wheat (Triticum aestivum L.), caused by the fungus Fusarium graminearum, is a major concern worldwide. FHB grain is reduced in yield, may fail to germinate, and is often contaminated with deoxynivalenol, a trichothecene mycotoxin linked to a variety of animal diseases and feed refusals. Annual losses in the tens of millions of dollars due to FHB underscore the need to develop improved methods of disease control and prevention. Previous research has identified deoxynivalenol biosynthesis as a virulence factor on wheat. Recently, we found that the TRI14 gene of F. sporotrichioides, closely related to F. graminearum, was not required for synthesis of a related trichothecene, T-2 toxin. TRI14 does not share similarity with any previously described genes in the databases. In this study, we examined the role that F. graminearum TRI14 may play in both deoxynivalenol synthesis and in virulence on wheat. TRI14 deletion mutants synthesize deoxynivalenol on cracked maize kernel medium and exhibit wild-type colony morphology and growth rate on complex and minimal agar media. However, FHB assays on greenhouse-grown wheat indicate that FgDeltaTri14 mutants cause 50-80% less disease than wild type and do not produce a detectable quantity of deoxynivalenol on plants. We discuss a number of possible roles that TRI14 may play in the disease process. 相似文献
942.
Sharpless KE Phinney CS Wood LJ Yen JH Howell DW 《Journal of agricultural and food chemistry》2003,51(23):6745-6751
Standard Reference Material (SRM) 2387 peanut butter was recently issued, and the process used for value assignment of nutrient and aflatoxin concentrations is reported herein. Values were assigned using data provided by the National Institute of Standards and Technology (NIST) and collaborating laboratories. SRM 2387 is intended for use as a primary material for assigning values to in-house control materials and for validation of analytical methods for measurements in peanut butter and similar high-fat matrixes. SRM 2387 lies in sector 3 of AOAC International's fat-protein-carbohydrate triangle. With the addition of SRM 2387, NIST now offers materials within-or on the borders between-all sectors of the triangle. The Certificate of Analysis for SRM 2387 provides assigned values for concentrations of fatty acids, proximates, elements, and total dietary fiber, for which product labeling is required by the Nutrition Labeling and Education Act of 1990, as well as several vitamins, amino acids, and aflatoxins, for which labeling is not required. (Aflatoxin levels in peanut butter are regulated by the Food and Drug Administration.) 相似文献
943.
944.
The legumes are an important group of flowering plants with a poorly documented evolutionary history. New fossil evidence provides data on the timing of the origin of the two derived subfamilies of legumes (the Mimosoideae and Papilionoideae). These data strongly suggest the importance of bee pollinators during a major period of angiosperm diversification. 相似文献
945.
946.
Parsons DW Li M Zhang X Jones S Leary RJ Lin JC Boca SM Carter H Samayoa J Bettegowda C Gallia GL Jallo GI Binder ZA Nikolsky Y Hartigan J Smith DR Gerhard DS Fults DW VandenBerg S Berger MS Marie SK Shinjo SM Clara C Phillips PC Minturn JE Biegel JA Judkins AR Resnick AC Storm PB Curran T He Y Rasheed BA Friedman HS Keir ST McLendon R Northcott PA Taylor MD Burger PC Riggins GJ Karchin R Parmigiani G Bigner DD Yan H Papadopoulos N Vogelstein B Kinzler KW Velculescu VE 《Science (New York, N.Y.)》2011,331(6016):435-439
Medulloblastoma (MB) is the most common malignant brain tumor of children. To identify the genetic alterations in this tumor type, we searched for copy number alterations using high-density microarrays and sequenced all known protein-coding genes and microRNA genes using Sanger sequencing in a set of 22 MBs. We found that, on average, each tumor had 11 gene alterations, fewer by a factor of 5 to 10 than in the adult solid tumors that have been sequenced to date. In addition to alterations in the Hedgehog and Wnt pathways, our analysis led to the discovery of genes not previously known to be altered in MBs. Most notably, inactivating mutations of the histone-lysine N-methyltransferase genes MLL2 or MLL3 were identified in 16% of MB patients. These results demonstrate key differences between the genetic landscapes of adult and childhood cancers, highlight dysregulation of developmental pathways as an important mechanism underlying MBs, and identify a role for a specific type of histone methylation in human tumorigenesis. 相似文献
947.
The recently discovered periodic comet Machholz 1986 VIII (1986e) travels closer to the sun than any known planet and any known comet with an orbital period of less than 150 years, thus providing astronomers with a unique object for studying cometary evolution. The comet is spiraling steadily closer to the sun, from perihelion distance q [unknown] 0.9 astronomical unit at about A.D. 700 to q [unknown] 0.13 at present (orbital period, 5.25 years), to an expected q [unknown] 0.03 by about 2450; should the comet survive such increasingly close perihelion passages, q will begin steadily to increase shortly thereafter. A review of observations made since discovery is presented, together with a discussion of numerical investigations of the comet's orbit over 4000 years and prospects for observing the upcoming return to perihelion in 1991. 相似文献
948.
Superconductivity above the temperature of liquid nitrogen in copper oxide-based systems has led to optimism that superconductors may at last find wide application. The critical temperature, however, is just one of the required parameters. The materials must be made into usable forms such as wire, thick films, thin films, and bulk ceramics. In addition, the critical current in these various forms is a crucial test of their utility. This article reviews the processing techniques used to fabricate potentially useful forms and assesses remaining problems. Considerable improvement in critical current density is necessary in bulk materials, and thin films need to be made compatible with other thin-film technology. 相似文献
949.
Two specimens of flint described by Green have been subjected to intensive x-ray fabric analysis. The specimen compressed in the beta-quartz field recrystallized to a fabric with a strong maximum of c-axes parallel to the direction of compression and a weaker equatorial concentration perpendicular to the axis of compression. Annealing of a similar specimen eliminated the equatorial concentration and strengthened the axial concentration six- to eightfold. In the latter specimen, the fabric determined by x-ray analysis agrees closely with Green's optically measured fabric. It is possible to obtain c-axis fabrics directly from the very weak 0003 diffraction peak. 相似文献
950.
Peres CA Baider C Zuidema PA Wadt LH Kainer KA Gomes-Silva DA Salomão RP Simões LL Franciosi ER Cornejo Valverde F Gribel R Shepard GH Kanashiro M Coventry P Yu DW Watkinson AR Freckleton RP 《Science (New York, N.Y.)》2003,302(5653):2112-2114
A comparative analysis of 23 populations of the Brazil nut tree (Bertholletia excelsa) across the Brazilian, Peruvian, and Bolivian Amazon shows that the history and intensity of Brazil nut exploitation are major determinants of population size structure. Populations subjected to persistent levels of harvest lack juvenile trees less than 60 centimeters in diameter at breast height; only populations with a history of either light or recent exploitation contain large numbers of juvenile trees. A harvesting model confirms that intensive exploitation levels over the past century are such that juvenile recruitment is insufficient to maintain populations over the long term. Without management, intensively harvested populations will succumb to a process of senescence and demographic collapse, threatening this cornerstone of the Amazonian extractive economy. 相似文献