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Dissecting the genetic basis of maize deep-sowing tolerance by combining association mapping and gene expression analysis 下载免费PDF全文
YANG Yue MA Yu-ting LIU Yang-yang Demar LYLE LI Dong-dong WANG Ping-xi XU Jia-liang ZHEN Si-han LU Jia-wen PENG Yun-ling CUI Yu FU Jun-jie DU Wan-li ZHANG Hong-wei WANG Jian-hua 《农业科学学报》2022,21(5):1266-1277
Deep-sowing is an important method for avoiding drought stress in crop species,including maize.Identifying candidate genes is the groundwork for investigating the molecular mechanism underlying maize deep-sowing tolerance.This study evaluated four traits(mesocotyl length at 10 and 20 cm planting depths and seedling emergence rate on days 6 and 12) related to deep-sowing tolerance using a large maize population containing 386 inbred lines genotyped with 0.5 million high-quality single nucleotide ... 相似文献
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为建立可应用于快速检测鹿茸及鹿血中布鲁氏菌的方法,保证鹿产品的药用、食用安全,试验根据布鲁氏菌特异性基因IS711设计合成引物和探针,建立实时荧光定量PCR方法,对反应条件进行优化,并绘制标准动力学曲线,Y=-3.14X+37.62,R2=0.997.结果 表明:该方法具有良好的重复性、敏感性和特异性,组内、组间重复性... 相似文献
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CHEN Yu-lu WANG Xin-yu ZHENG Shu-zhan DU Yan-fei YE Qiang LI Jia-fu FAN Zhong-cai LI Guang 《园艺学报》2000,36(8):1531-1536
AIM To observe the effect of recombinant mouse interleukin-11 (rmIL-11)injected subcutaneously into mice on heart structure and function and to determine its pro-fibrotic effect. METHODS C57BL/6 mice were randomly divided into experimental group and control group.The mice in experimental group were injected subcutaneously with recombinant mouse IL-11 at the dose of 100 μg·kg-1·d-1 for 3 consecutive weeks, while the control group were given equal volume of normal saline in the same way. After the experiment was finished, the parameters of heart function were measured by echocardiography.The heart weight was weighed and the cardiac weight index (CWI) was calculated. HE staining and Masson's trichrome staining were performed to observe the pathological changes and the extent of myocardial fibrosis in mouse myocardia respectively, and the cardiac collagen volume fraction (CVF) was calculated. The expression levels of extracellular matrix proteins in the myocardial tissues of mice, including type Ⅰ collagen, type Ⅲ collagen and fibronectin, were determined by Western blot. RESULTS Left ventricular ejection fraction and left ventricular fraction shortening in experimental group were obviously lower than those in control group (P <0.01), however left ventricular end-diastolic diamension and left ventricular end systolic dimension were significantly higher than those in control group (P <0.05).Compared with control group, the CWI was increased (P <0.01), the myocardial arrangement was disorder, the necrosis of cardiac myocytes was increased, and excessive deposition of collagen was observed in the myocardial tissues in experimental group. Correspondingly, the CVF and protein levels of type Ⅰ collagen, type Ⅲ collagen and fibronectin in the left ventricle in experimental group were increased significantly (P <0.05). CONCLUSION Injection of rmIL-11 into the mice subcutaneously induces fibrogenesis in the heart, which implies that IL-11 is likely a novel pro-fibrotic factor. 相似文献
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AIM To study the effect of dihydroartemisinin (DHA) on the radiotherapy efficiency in hepatocellular carcinoma H22 cell tumor-bearing mice and the role of phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway in this process. METHODS A model of H22 cell tumor-bearing mice was established. The mice was divided into model group, single radiotherapy group, 5-fluorouracil (5-FU) group, and low-, medium- and high-dose DHA groups. The body weight and tumor volume in each group were measured every other day. At the end of administration, blood was collected from the tail of the mice and the animals were killed by neck removal immediately. The synergistic effect of DHA on radiotherapy was determined, and tumor growth inhibitory rate was calculated. The degree of lymphocyte transformation and natural killer (NK) cell activity were measured by MTT, the serum levels of interleukin-2 (IL-2) and IL-4 were measured by ELISA, and the protein levels of PI3K, AKT and p-AKT were determined by Western blot. RESULTS The H22 cell tumor-bearing mouse model was successfully constructed. Compared with model group, the TGT3 (tumor growth time to reach 3 times of volume) of single radiotherapy group was remarkably increased (P <0.05), while tumor weight, lymphocyte transformation degree, NK cell activity, IL-2 and IL-4 levels, PI3K protein level and AKT phosphorylation level were remarkably decreased (P <0.05). Compared with single radiotherapy group, TGT3, EF (enhancement factor), tumor inhibitory rate, lymphocyte transformation degree, NK cell activity, IL-2 level and IL-4 level were increased with the increase in DHA dose (P <0.05), and the PI3K protein level and AKT phosphorylation level were decreased (P <0.05). CONCLUSION DHA may enhance the immunity of tumor-bearing mice by inhibiting the activity of PI3K/AKT signaling pathway, thereby enhancing the efficacy of radiotherapy. 相似文献
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花色苷含量是荔枝果实呈现鲜红色的重要次生代谢产物,前人研究结果表明,LcMYB1通过调控关键基因的表达而影响荔枝果皮中花色苷的积累,其中LcDFR和LcUFGT1是荔枝果皮花色苷生物合成的关键基因,但是LcMYB1如何影响基因的表达,是否与结构基因启动子结合以及具体结合区段目前还不清楚。本研究通过克隆和分析LcDFR和LcUFGT1的启动子区域并对其进行分段处理,利用双荧光素酶和酵母单杂交实验,探究LcMYB1与LcDFR和LcUFGT1的启动子的结合区域。结果表明,LcDFR起始密码子上游1927 bp和LcUFGT1起始密码子上游1584 bp的启动子上分别有3个可能的MYB结合位点;双荧光素酶和酵母单杂交实验均显示LcMYB1可以结合LcDFR基因起始密码子上游904 bp到1425 bp包含有1个MYB-CORE元件的区域,LcMYB1与LcUFGT1基因启动子结合的区域是起始密码到上游610 bp,此区域包含有2个MYB-CORE元件。研究结果进一步证实了LcMYB1通过与基因LcDFR和LcUFGT1启动子结合发挥调控作用,并缩小了结合位点的范围。 相似文献
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根据金黄色葡萄球菌特异性STAA-Aul基因序列,设计、合成了一对引物,运用聚合酶链式反应(PCR)技术从10头患有奶牛乳房炎的奶牛奶样中扩增得到8个大小为420 bp的DNA产物;阴性样品中未扩增到产物。测定了其中2个DNA片段的核酸序列,确定为金黄色葡萄球菌的STAA-Aul基因。 相似文献
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体外研究了不同质量浓度5、20、40 mg/L棉酚对瘤胃厌氧真菌和瘤胃细菌发酵稻草活力的影响。瘤胃真菌发酵试验中,各处理组的底物消失率均显著低于对照组(0)(P<0.05)。产气量结果表明,20、40 mg/L两试验组的发酵明显滞后于其它各组,且发酵24 h和48 h的累计产气量显著低于对照组及51、0 mg/L试验组(P<0.05),说明20 mg/L和40 mg/L的棉酚可显著抑制瘤胃真菌的发酵。在瘤胃细菌发酵试验中,51、0 mg/L棉酚处理组的底物消失率与对照组(0)无明显差异,而20和40 mg/L棉酚处理组的底物消失率显著低于对照组(P<0.05)。累计产气量结果表明,各浓度水平的棉酚对瘤胃细菌发酵无明显的滞后效应。因此,瘤胃真菌对棉酚较为敏感,而瘤胃细菌对棉酚有较强的耐受能力,但高浓度的棉酚对细菌发酵活力有一定的抑制作用。 相似文献