苹果锈果类病毒辽宁分离物的克隆与序列分析

 类病毒是迄今为止发现的最小病原物,是一类单链共价闭合环状的裸露RNA分子,由246~401个核苷酸组成,具有自我复制能力,是感染某些高等植物的低分子致病性因子。目前已确定的果树类病毒有18种,分属于马铃薯纺锤块茎类病毒科(Pospiviroidae)和鳄梨日斑类病毒科(Avsunviroidae)。     

黄河兰州段多环芳烃生态风险的初步评价

以黄河兰州段11个不同采样点3种多环芳烃的临测浓度及其对6～38种水生生物的LC50为基础资料,分别应用商值法、概率密度函数重叠面积和联合概率曲线3种风险浮价方法对黄河兰州段苯并(a)芘、荧蒽、芘的生态风险进行了评价.结果表明:黄河兰州段3种PAHs残留具有一定的生态风险.其中低暴露风险条件下(受威胁生物不超过1%),...     

Infection of human enterovirus 71 in immunosuppressed rhesus monkeys

AIM: To investigate the infection process of human enterovirus 71 (EV71) in a rhesus monkey model of immunosuppression.METHODS: The monkey immunosuppression model was established by oral administration of cyclosporin A at a daily dose of 15 mg/kg for 7 days. Fourteen days after inoculation of EV71 FY23 strain via respiratory and digestive tracts, the infectious monkeys were sacrificed, and then the pathological changes of systemic organs were observed and the viral analysis in different tissues was performed.RESULTS: Compared with the experimental group without cyclosporin A, more positive results were observed in the immunosuppressed monkeys, such as more severe clinical symptoms, higher viral load/antigen expression and more pathological changes in the organs.CONCLUSION: The findings of the viral multiplication in the host provide evidence for evaluation of EV71 vaccine, and also indicate the delimitation for the population using EV71 vaccine in future.     

含笑新品种‘梦星’

‘梦星’是以乐昌含笑(Michelia chapensis Dandy)为母本,紫花含笑(M.crassipes Law)为父本杂交选育出的新品种。常绿乔木,叶片薄革质,长圆状倒卵形,先端渐尖,内轮花被片紫红色,色晕较深,外轮较浅,雄蕊群紫红色,花期3—4月,喜温暖湿润气候,可在江南各地广泛种植。     

嫁接对西瓜根际微生物种群数量及群落结构的影响

分别采用微生物培养的方法、16S rDNA PCR-RFLP方法和phl D基因PCR-RFLP方法研究了嫁接对西瓜根际微生物种群数量、细菌群落结构及拮抗菌（2, 4-DAPG产生菌）群落结构的影响。结果表明：① 嫁接西瓜根际细菌和真菌数量有所提高,而放线菌数量则有所减少。② 嫁接西瓜与自根西瓜具有1个相同的主要根际细菌基因型;不同砧木嫁接的西瓜与自根西瓜也分别具有不同基因型的细菌。③ 嫁接西瓜具有与自根西瓜不同的主要根际拮抗菌基因型。     

芋优异种质资源鉴定评价研究

对农业行业标准《农作物种质资源鉴定评价规范芋》编制的技术路线进行了分析研究,以期为芋优异种质资源的鉴定评价提供参考。     

我国甜菜抗逆性研究进展

从盐、水、温度、病害等方面综述了甜菜抗逆性研究进展,并对其研究前景进行了展望。     

Increased expression of CD40 ligand by peripheral blood mononuclear cells in patients with systemic lupus erythematosus

AIM:To investigate expression and function of CD40 ligand by peripheral blood mononuclear cells (PBMCs) in patients with systemic lupus erythematosus (SLE).METHODS:Expression of CD40 ligand by PBMCs in patients with SLE and control were examined by flow cytometric analysis before and after stimulated by phytohemagglutinin(PHA)and depressed by Dexamethasone(Dex). The correlation between expression of CD40 ligand and SLE activity index(SLEDAI) was analysed in patients with SLE.RESULTS:The expression of CD40 ligand by PBMCs in patients with active SLE was higher than that in patients with inactive SLE and control. Though the expression of CD40 ligang by PBMCs could be stimulated by PHA in three groups, it was the highest in patients with active SLE. Dex depressed the expression of CD40 ligand by PBMCs significantly in patients with SLE, but not in control. There was high positive correlation between expression of CD40 ligand and SLEDAI in patients with active and inactive SLE.CONCLUSION:Increased expression of CD40L by PBMCs in patients with SLE may play an important role in pathogenesis of SLE.     

低浓度玉米小斑病菌C小种毒素培养滤液对玉米叶片过氧化物酶活性的诱导作用

分别用不同低浓度玉米小斑病菌C小种毒素培养滤液处理两种基因型(C103和B37)的同核异质系的玉米叶片,以提高玉米叶片内过氧化物酶(POD)的活性;结果说明:过氧化物酶的活性变化与植物抗病性呈正相关,低浓度C毒素培养滤液本身能够作为激发子来诱导玉米获得抗性,故低浓度C毒素培养滤液作为激发子来诱导玉米的系统获得性抗性具有一定的广适性。对CB37和CC103的处理中,1∶60处理组效果均表现为较好,POD酶活性最高。     

云南高海拔粳稻区白叶枯病菌致病型的鉴定

水稻白叶枯病是危害水稻的重要细菌性病害之一,病原为稻生黄单胞菌水稻致病变种Xanthomonas oryzae pv.oryzae (Xoo).近10年来云南省水稻白叶枯病的危害向高海拔粳稻区扩散,在海拔2200m的剑川县,白叶枯病已成为最严重的水稻病害[1-2].开展高海拔粳稻区白叶枯病菌致病型的鉴定、分布和分化研究,对云南高海拔粳稻区稻作生产具有指导意义.