Prokaryotic Expression and Antigenicity Analysis of Porcine Japenese Encephalitis Virus Envelope Protein Domain Ⅲ

In order to analyze the antigenicity of porcine Japanese encephalitis virus (JEV) E protein domain Ⅲ, which was expressed by pET-28a vector with His-tag and purified through Ni-NTA, the BALB/c mice were immunized with the purified protein.We identified the antigenicity of domain Ⅲ of E protein and the anti-mice and anti-porcine JEV E Ⅲ protein specific antibody titers by SDS-PAGE, Western blotting, indirect ELISA and IFA.SDS-PAGE results showed the expressed target protein existed mainly in the form of inclusion body.Western blotting, ELISA test results showed that the protein had good reactivity with anti-serum.The mice immunized with the purified JEV E Ⅲ protein generated 1×10⁵ anti-JEV E Ⅲ protein specific antibody titers by ELISA, and the porcine immunized with the porcine JEV generated 5.1×10⁴ anti-JEV specific antibody titers.The IFA results showed that JEV E Ⅲ protein anti-serum could identify JEV antigen.The above results showed that the recombinant JEV E Ⅲ had good antigenicity.These results provided important basis for development of diagnostic antigen for JEV.     

苏北沿海滩涂马齿苋高效栽培技术

根据江苏苏北沿海滩涂地区的气候、土壤等因素,结合几年的田间试验,总结了江苏苏北沿海滩涂地区马齿苋高产栽培的适宜播种量、施肥、病虫害防治、采收等栽培管理措施。     

不同性能薄膜对草莓气调保鲜效果的影响

以PE/OPP/PE复合膜、PE膜及电晕处理PE膜为试材,采用与空气相同(21%O_2+0.9%CO2)的初始气体比例被动气调包装件,研究不同透气、透湿性能的薄膜对草莓气调保鲜效果的影响。结果表明:在室温(15±2)℃、相对湿度33%~35%的条件下,在草莓6d贮存期内,PE/OPP/PE复合膜保持了草莓较高的感官评价和硬度,电晕处理PE膜更有利于延缓草莓可溶性固形物含量的下降及pH的升高。故PE/OPP/PE复合膜和电晕处理PE膜对草莓的保鲜效果优于PE膜。     

Nonlinearity of Electronic Throttle and Its Control

This paper introduces the development of the electronic throttle control system (ETCS) , the structure and the principle of the system are also involved. After analyzing the nonlinearity problem existing in the mechanical structure of the system, a mathematical model of the ETCS is built up. Then three controllers (PID controller, Fuzzy controller, sliding mode controller) were designed and simulated in the matlab simulink. The result indicates that the PID controller has an overshoot,but the Fuzzy controller and the sliding mode controller can settle the nonlinearity problem better.     

Portable ECG Monitor Front- ended Design with C8051 F020

The electrocardiogram (ECG) signal is a recording of the electrical activity on the body surface generated by the heart frequency of which is weak and low. Many researchers devote themselves to get more perfect ECG in many ways. This article introduces the characterize of an ECG signal , then discusses the requirements for the sampling circuit of an ECG. Since there are some shortcoming in common design means, the authors give an effective circuit. The system is composed of a low-cost , low-power and high-performance MCU C8051F020 , amplifier AD620 , right-leg drive circuit ,communication circuit and power management circuit. Then software design is given which is used to sampling and filtering ECG signal. Levkov filter arithmetic and smooth filter arithmetics are used in this design. In the end , the authors advance a distributing and networking ECG monitor system.     

0.6m3水力碎浆机传动系统的设计

在水力碎浆机的设计过程中,传动系统的设计非常关键,因传动系统的设计直接决定了整台机器的性能、效率。传动系统将转距由电动机通过带轮传动、主轴传送至转盘、刀片,转盘的回转形成浆流的循环和物料的摩擦,加之转盘及槽体上安装的刀片的碎解作用,从而把物料碎解成浆。0.6m3水力碎浆机是专为造纸实验室而设计,该产品传动系统采用转子传动装置 皮带传动 立式电动机的结构形式,主要包括主轴、大皮带轮、轴承、轴承座、受浆盘和转盘等部件。与传统水力碎浆机设计不同的是,0.6m3水力碎浆机槽体内不设计传动结构,动力消耗少,运行平稳。     

温室雨水收集系统的应用研究

根据雨水资源的现状。对我国温室雨水资源进行了分析。为了提高对雨水的利用。提出了利用温室雨水收集系统收集雨水用于温室灌溉．并对系统的经济性及应用前景作了分析。     

上海青浦章浜河泵闸自动控制系统的设计

结合上海市青浦区章浜河泵闸系统的设计，提出了泵闸自动控制设计方案的新思路，该方案对中小型泵闸而言，具有结构简捷，运行可靠和性能价格比高的特点，泵闸运行实践表明，系统完全达到了设计要求，适宜于在中小型泵闸领域推广。