石家庄市园林植物蚧壳虫种类调查初报

通过随机抽样调查的方法,采集了石家庄市为害园林植物的蚧壳虫样本,并对其种类进行了鉴定。结果表明:石家庄市为害园林植物的蚧壳虫种类主要有7科(蜡蚧科、绒蚧科、粉蚧科、盾蚧科、绵蚧科、蚧科和珠蚧科)17种,其中,草履蚧、卫矛失尖盾蚧、桑白盾蚧、白蜡囊粉蚧、紫薇绒蚧和日本龟蜡蚧等对园林植物为害相对较严重。同时,对蚧壳虫的防治技术进行了探讨,以期为石家庄市园林绿化工作中蚧壳虫的防治提供有益参考。     

抗寒锻炼期间三种楸树叶片激素含量变化

在抗寒锻炼期间,以梓楸、滇楸、灰楸3种楸树为材料,利用酶联免疫吸附方法(ELISA)测定其叶片内源激素IAA(吲哚乙酸)、ABA(脱落酸)、GA3(赤霉素)、ZR(玉米核苷)的含量。结果表明:抗寒锻炼期间,3种楸树叶片ABA含量基本上均呈上升趋势,其它3种激素含量变化趋势不一致。说明在抗寒锻炼期间,3种楸树的抗寒能力逐渐增加。     

豫中浓香型烟区烤烟替代品种豫烟5号、豫烟7号特性研究

为了找到最具浓香型特色的烤烟替代品种,2008年在河南省襄城县种植了豫烟5号、豫烟7号。以常规品种中烟100作为对照,调查各品种长势长相、物理性状、化学性状、经济性状、外观质量及感官评吸质量,结果表明,豫烟5号、豫烟7号与对照相比,具有长势强,上部叶叶质重低,氯含量低,总糖含量高,钾氯比值大,产量高,外观质量好,评吸结果总分高等优势,但也有下部叶叶质重低,含梗率高,上部叶钾含量较低等缺点。综合比较,豫烟5号与豫烟7号烟叶品质优于对照中烟100,但豫烟7号不易烘烤,需要进一步研究配套烘烤技术。     

Effect of EGFP gene transfection on the cell cycle distribution of primary cultured human chondrocytes

AIM: To investigate the effect of enhanced green fluorescence protein (EGFP) gene transfection on the cell cycle distribution of primary cultured human chondrocytes in order to establish a tracking method of cultured human nasoseptal chondrocytes. METHODS: pEGFP-N1 plasmid was amplified in E.coli, and purified by high purity kit. Primary cultured human chondrocytes,which were initially obtained from the nasoseptal cartilage, were cultured in vitro and transferred with pEGFP-N1 by means of electroporation with Amaxa nucleofector device. Transfering process and transient expression were evaluated by laser scanning confocal microscope (LSCM), the transfer efficiency and the cell cycle distribution were evaluated by flow cytometry. RESULTS: There was significant expression of EGFP at 24 h after transferring. The transfection efficiency of pEGFP-N1 into primary cultured human chondrocytes reached 35.37％ at 48 h. It didn't affect the process of cell adherance and had no effect on the cell cycle distribution. CONCLUSION: Primary cultured human chondrocytes, which were transfected with pEGFP, are alive in vitro, and the transferring process doesn't affect the cell cycle distribution. These results suggest that pEGFP-N1 is an ideal transient expression vector for primary cultured human chondrocytes and it might be a well tracer in construction tissue engineered cartilage.     

Effects of Yangxue-Jiedu decoction on imiquimod-induced psoriasis-like lesions in STAT3 transgenic mice

AIM: To observe the effects of Yangxue-Jiedu (YXJD) decoction on imiquimod-induced psoriasis-like lesions in STAT3 transgenic mice.METHODS: STAT3 transgenic mice (n=24) were randomly divided into 4 groups: control group (using purified water for oral administration), model group (topical 5% imiquimod 42 mg and using purified water for oral administration), YXJD groups (topical 5% imiquimod 42 mg and using YXJD decoction for oral administration), and methotrexate (MTX) group (1 mg/kg MTX solution for oral administration, with the same topical imiquimod as model group). On day 7, the skin lesions were collected for examination. The lesions were evaluated according to the psoriasis area and severity index (PASI). The skin barrier function was evaluated by assessing oil and water components in the skin. The inflammation of psoriasis-like lesions was assessed by histological method. The expression of proliferating cell nuclear antigen (PCNA) and CD3 was assessed by immunohistochemical staining. The mRNA expression of IL-17A, IL-17C, IL-22 and RORγt was detected by real-time PCR. The levels of JAK/STAT3 pathway-related proteins in isolated T cells were determined by Western blot.RESULTS: Administration of YXJD decoction inhibited imiquimod-induced keratinocyte proliferation and infiltration of CD3⁺ T cells in psoriatic lesions, and ameliorated the epidermal barrier by up-regulation of the oil and water components in psoriatic lesions. Meanwhile, administration of YXJD decoction improved the systemic immune responses by reducing the weight of the spleen. The inflammatory cytokines IL-17A, IL-17C, IL-22 and RoRγt, and the levels of JAK/STAT3 pathway-related proteins STAT3, p-STAT3, JAK3 and p-JAK3 were decreased by administration of YXJD decoction.CONCLUSION: YXJD decoction likely alleviates imiquimod-induced psoriasis-like lesions in the STAT3 transgenic mice by inhibiting the phosphorylation of STAT3, and reducing the expression of IL-17A, IL-17C, IL-22 and RORγt.     

深层灌水对冬小麦耗水特性及水分利用效率的影响

以高产中晚熟冬小麦品种良星99为材料,在运城市盐湖区山西水利职业技术学院实训基地进行田间试验,研究了深层灌水对冬小麦耗水特性和水分利用效率的影响。结果表明:整个生育期,深层灌水处理根区20~160 cm土层土壤水分动态变化比地表灌处理明显;T1(地表灌水)处理总耗水量最大,显著高于T2(湿润层深度为根系60%)、T3(湿润层深度为根系75%)和T4(湿润层深度为根系90%),深层灌水增加了降雨和灌溉水的消耗,降低了土壤贮水的消耗;T2和T3处理间无显著差异,T3在抽穗至灌浆期末、灌浆至成熟期的耗水量和耗水模系数均较大;不同湿润层深度条件下,T1处理水分利用效率和产量最低,随湿润层深度增加,其他处理水分利用效率呈先增加后降低的趋势。湿润层深度为150 mm和188 mm的T2和T3产量、水分利用效率和灌溉水利用效率表现最好,T1处理最低。T3为本试验条件下高产节水的最佳处理。     

长白忍冬株间种子性状的观测与分析

以吉林省集安产地的长白忍冬为材料,观测株间种子性状,结果表明:株间种子大小存在差异,长度值变动于3.0～5.0 mm之间,平均值为4.2 mm;宽度值变动于2.0～3.0 mm之间,平均值为2.8 mm.种子扁状椭圆形,发芽处微尖,一面有纵向微凸起,种皮无光泽,相对光滑,株间无差异.株间种子千粒重变化范围在4.4～6....     

NO、TNF和IL-2与新型鸭肝炎病毒感染雏鸭肝脑组织损伤的关系

用新型鸭肝炎病毒人工感染9日龄健康樱桃谷雏鸭，对感染后12、24、48、96、168h和14d雏鸭血液、肝和脑组织中一氧化氮（NO）含量，血液中肿瘤坏死因子（TNF）和白细胞介素2（IL-2）含量进行了测定，同时对感染雏鸭的组织病理学变化进行了观察。结果表明．血清中NO含量在接种后48h开始升高，一直持续到接种后96h，接种后7d恢复正常；肝脏组织中NO含量仅在接种后24h与对照组比较显著升高，在其他时间未表现有差异；脑组织中NO含量在整个试验期间没有变化。血清中的TNF和IL-2含量在接种后24h均表现升高，接种后96h降低，其他时间无改变。感染雏鸭的肝组织在接种后24h表现出血性坏死性肝炎变化，接种后48～96h呈增生性病变，而接种后各时期脑组织均呈非化脓性脑炎变化。由此表明，新型鸭肝炎病毒感染可导致雏鸭体内NO、TNF和IL-2发生变化，并且与肝组织损伤、疾病的发生发展有关。     

不同繁殖季节绵羊卵巢组织中piRNAs序列特征及表达差异分析

为了分析绵羊卵巢组织中piRNAs的序列特征及不同繁殖季节绵羊卵巢组织中piRNAs的表达差异,本试验采用Solexa测序技术对休情季节（休情期）和发情季节（卵泡期和黄体期）滩羊卵巢组织进行高通量测序,采用生物信息学方法筛选绵羊卵巢中的piRNAs,对piRNAs的长度分布、首位碱基偏好、基因组来源、染色体分布、链特异性、ping-pong循环特征、piRNAs比对重复序列类型进行分析,并对发情季节与休情季节绵羊卵巢之间差异表达的piRNAs进行靶基因预测及其靶基因的GO功能和KEGG Pathway富集分析。结果显示,绵羊卵巢中不同长度的piRNAs首位碱基偏好性不一致;3个时期（休情期、黄体期和卵泡期）的piRNAs均主要比对到基因内含子、编码区及lncRNA区域,而比对到重复序列的piRNAs相对较少;绵羊卵巢piRNAs在染色体上呈不均匀分布;绵羊piRNAs簇存在很强的链特异性,但首位碱基偏好性不明显;3个时期的piRNAs均无明显的ping-pong循环特征。KEGG富集分析发现,差异piRNA靶基因主要富集在RNA运输通路（RNA transport pathway）、嘌呤代谢通路（purine metabolism pathway）、黏着斑通路（focal adhesion）、PI3K-Akt信号通路（PI3K-Akt signaling pathway）、Hippo信号通路（Hippo signaling pathway）等与绵羊繁殖相关的通路。本研究揭示了不同繁殖季节绵羊卵巢组织中的piRNAs序列特征,暗示绵羊卵巢组织中的piRNAs可能通过BAD等基因和黏着斑通路等调控绵羊季节性繁殖,为深入解析绵羊季节性发情分子机制提供一定参考。     

女性身体·五四话语·革命救赎

在革命意识形态成为主流话语左右着文学创作时,丁玲试图通过对女性身体的革命救赎寓言化书写将女性书写融入主流话语体系之中。女性主体意识在这一过程中被深度扼制。然而,女身书写的自身逻辑却在另一层面干扰着文本意义的生成,不自觉地流露出作者未被泯灭的女性意识。