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11.
We describe a new molecular class of genetic-pairing system that has a native DNA backbone but has all four base pairs replaced by new, larger pairs. The base pairs include size-expanded analogs of thymine and of adenine, both extended by the width of a benzene ring (2.4 A). The expanded-diameter double helices are more thermodynamically stable than the Watson-Crick helix, likely because of enhanced base stacking. Structural data confirm a right-handed, double-stranded, and base-paired helical form. Because of the larger base size, all the pairs of this helical system are fluorescent, which suggests practical applications in detection of natural DNA and RNA. Our findings establish that there is no apparent structural or thermodynamic prohibition against genetic systems having sizes different from the natural one. 相似文献
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ObjectiveTo investigate the contribution of K+ channels on peripheral antinociception induced by ketamine.Study designProspective experimental study.Animals110 male Wistar rats weighing 160–200 g.MethodsThe paw pressure required to elicit limb flexion was designated as the nociceptive threshold. Hyperalgesia was induced by intraplantar injection of prostaglandin E2. All drugs were administered locally into the right hind paw of rats. Ketamine was administered into the right hind paw 2 hours and 55 minutes after local injection of PGE2. Tetraethylammonium was administered 30 minutes prior to ketamine and the other K+ channel blockers, glibenclamide, dequalinium and paxilline, were administered 5 minutes prior to ketamine.ResultsProstaglandin E2 (2 μg per paw) induced hyperalgesia. Ketamine (10, 20, 40 and 80 μg per paw) elicited a local peripheral antinociceptive effect that was antagonized by a specific blocker of ATP‐sensitive K+ channels, glibenclamide (20, 40 and 80 μg per paw). In another experiment, the non‐selective voltage‐dependent K+ channel blocker tetraethylammonium (30 μg per paw) and small and large conductance blockers of Ca2+‐activated K+ channels, dequalinium (50 μg per paw) and paxilline (20 μg per paw), were ineffective at blocking the effect of a local ketamine injection.Conclusions and clinical relevanceAnalysis of these results provides evidence that ketamine, may in part, induce peripheral antinociceptive effects by ATP‐sensitive K+ channel pathway activation. 相似文献
14.
D C Baker L F James W J Hartley K E Panter H F Maynard J Pfister 《American journal of veterinary research》1989,50(8):1396-1399
Three groups of 5 pigs each were fed a high selenium (Se) diet by mixing either Astragalus praelongus (31.6 ppm Se in feed), A bisulcatus (31.7 ppm Se in feed), or sodium selenate (26.6 ppm Se in feed) with commercial hog feed. Ten control pigs were fed only commercial hog chow containing trace selenium (0.44 ppm Se). Pigs were fed for 9 weeks and necropsied when they had ataxia or paralysis. Blood was collected for hematologic and serum biochemical determinations, and samples of various tissues were collected and fixed in neutral-buffered 10% formalin for histologic evaluation or frozen for determination of selenium concentration. All forms of selenium induced clinical signs of weight and hair loss, with cracked hooves and inflamed coronary bands developing in all Na2SeO4-fed pigs and 1 A praelongus-fed pig, but not in A bisulcatus-fed pigs. Serum calcium, phosphorus, and albumin concentrations were unchanged or significantly decreased from prefeeding values in groups fed selenium. Serum aspartate transaminase (AST) activities in Astragalus species-fed groups, and amylase activities and PCV in all groups of pigs fed selenium, were increased. Serum alkaline phosphatase and creatine kinase activities were significantly increased in the A praelongus-fed pigs and significantly decreased in Na2SeO4-fed pigs. Terminal tissue and body fluid selenium concentrations were determined in all groups of pigs fed selenium and compared with values in control pigs. Urine and bile concentrations were increased by the greatest factor (40 to 100x), with tissue concentrations of selenium increased by a lesser factor (6 to 17x).(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
15.
NH Bonadeo J Erland D Gammon D Park DS Katzer DG Steel 《Science (New York, N.Y.)》1998,282(5393):1473-1476
Picosecond optical excitation was used to coherently control the excitation in a single quantum dot on a time scale that is short compared with the time scale for loss of quantum coherence. The excitonic wave function was manipulated by controlling the optical phase of the two-pulse sequence through timing and polarization. Wave function engineering techniques, developed in atomic and molecular systems, were used to monitor and control a nonstationary quantum mechanical state composed of a superposition of eigenstates. The results extend the concept of coherent control in semiconductors to the limit of a single quantum system in a zero-dimensional quantum dot. 相似文献
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Cumulative Effect of Annual Additions of Uncomposted Oak and Maple Leaves on the Yield of Vegetables
Abigail A. Maynard 《Compost science & utilization》2018,26(2):98-103
For 22 years, plots were amended annually with oak (Quercus velutina Lam.) or sugar maple leaves (Acer saccharum Marsh.) in Mt. Carmel, CT on a loamy upland soil. Leaves were incorporated into the soil immediately after application in the fall. Yields of 12 different vegetable crops (lettuce, peppers, eggplant, tomatoes, edamame, delicata squash, spaghetti squash, onions, leeks, rutabaga, carrots, and beans) from these plots were compared to yields from unamended controls. All plots received equal amounts of fertilizer. There were no significant differences in vegetable yields with any of the crops between any of the treatments indicating there are no deleterious effects of annual applications of uncomposted leaves. Some soil characteristics such as organic matter and pH improved on the leaf amended plots. Study shows that annual applications of leaves to cropland (sheet composting) is a viable alternative for the disposal of leaves. 相似文献
18.
Abigail A. Maynard 《Compost science & utilization》2013,21(4):38-44
This three year study was conducted to evaluate the suitability of municipal solid waste (MSW) compost as a soil amendment or a mulch to suppress weeds in the production of field grown nursery stock. In the soil amendment experiment, compost was applied at the rates of 0, 56, and 112 t/ha and incorporated into the soil prior to planting. In the mulch/weed control experiment, 224 t/ha of compost was applied to one set of plots as a mulch after planting and compared to plots treated with herbicide and untreated controls. Each plot in both experiments contained four rows with one each of red maple (Acer rubrum L.), sugar maple (Acer saccharum Marsh.), white pine (Pinus strobus L.), and pin oak (Quercus palustris Muenchh.). Mortality and weed counts were determined during the first growing season. Annual measurements of crown height, crown width, and caliper (at the 1 foot level) were made the following three winters. Two inches of compost mulch (224 t/ha) provided adequate weed control only in the first year. Mowing suppressed the weeds that invaded the plots beyond the first year. For maples, first year mortality in plots mulched with 224 t/ha compost or amended with 112 t/ha compost was significantly less than unamended controls. In subsequent years, the mulched maples grew more than trees in the unamended controls. In the plots amended with 56 or 112 t/ha compost, sugar maples had increased height, canopy, and caliper growth compared to the unamended controls. The hearty pin oak saplings survived transplanting and there was a significant increase in caliper growth in plots amended with 112 t/ha (1 inch) of compost. Mortality of white pine increased when mulched with 224 t/ha compost. Increased mortality was most likely due to high concentrations of ammonium and soluble salts in the immature compost. Beyond the first year, there were no negative impacts on the growth of white pine from any of the compost treatments. 相似文献
19.
Abigail A. Maynard 《Compost science & utilization》2013,21(3):203-209
For two consecutive years, one-inch (50 T/A) of leaf compost was applied to plots on a sandy terrace soil (Windsor, Connecticut) and a loamy upland soil (Mt. Carmel, Connecticut). These compost-amended plots were fertilized with 10-10-10 (N-P2O5-K2O) at three rates: 0, 650 (half), 1300 (full) lb/A and cottonseed meal at a rate of 2166 lb/A. Tomato (Lycopersicon esculentum Miller) yield from compost-amended plots were compared to yield from unamended control plots fertilized with 1300 lb 10-10-10/A. In both years, at both sites, plots only amended with compost had yields equivalent to the fertilized control plots. In both years, the greatest yields at Mt. Carmel were from plots amended with compost and the full rate of inorganic fertilizer. In the second year, yields from compost-amended plots fertilized at half the rate were equivalent to compost-amended plots fertilized at full rate. The yields from the organic plots were similar to the control plots the first year and to plots amended with compost and half the rate of fertilizer the second year. At Windsor, the greatest yields for both years were from plots amended with compost and the full rate of fertilizer. The compost-amended plots fertilized with cottonseed meal produced the lowest yields, both years. Cumulative effects of compost on soils were measured by increases in pH and organic matter percentage at both sites. Tomato fruit in plots amended with compost and no fertilizer developed less blossom-end rot than fruit in all other treatments. 相似文献
20.
Chlamydiosis in workers at a duck farm and processing plant 总被引:4,自引:0,他引:4
DG HINTON A. SHIPLEY† JW GALVIN‡ JT HARKIN‡ RA BRUNTON† 《Australian veterinary journal》1993,70(5):174-176
SUMMARY An outbreak of chlamydiosis was Investigated in workers at a duck farm and processing plant during winter 1989. Chlamydia psittaci was isolated from ducks, but there was little evidence of clinical chlamydiosis in the ducks. Serological tests showed that 76% of workers had been exposed to Infection, 12% recently, 16% had not been exposed and 8% had inconclusive test results. There was a strong association (P < 0.05) between years of employment and exposure to Infection. Infection appeared to be contracted during the first year of employment. Measures were Implemented to reduce shed contamination and control rodents and wild birds. At the same time, ducks were medicated, the processing plant was modified, work practices were altered and workers were advised about chlamydiosis to reduce the risk of human infection. 相似文献