Historical spatial baselines in conservation and management of marine resources

Increased knowledge on the spatial distribution of marine resources is crucial for the implementation of a true ecosystem approach to management and the conservation of marine organisms. For exploited fish species characterized by aggregation behaviour during spawning time, the identification and tracking of spawning areas is essential for a correct assessment of their productivity and population abundance. To elucidate this concept, we reconstructed the spatio‐temporal distribution of adult plaice (Pleuronectes platessa, Pleuronectidae) during spawning time along the 20^th century. Historical data reveal that not only the abundance but also the former population richness was much higher than previously estimated and has declined because of protracted over‐exploitation during the last 30 years. We conclude that forecast of stock recovery to former levels of abundance neglecting spatial reorganizations might be over‐optimistic and shaded by a lost memory of the past population richness. These results reinforce the importance of managing exploited marine resources at a greater spatial resolution than has been carried out in the history of fishery management.     

Effects of transition season management on soil N dynamics and system N balances in rice–wheat rotations of Nepal

In the low-input rice–wheat production systems of Nepal, the N nutrition of both crops is largely based on the supply from soil pools. Declining yield trends call for management interventions aiming at the avoidance of native soil N losses. A field study was conducted at two sites in the lowland and the upper mid-hills of Nepal with contrasting temperature regimes and durations of the dry-to-wet season transition period between the harvest of wheat and the transplanting of lowland rice. Technical options included the return of the straw of the preceding wheat crop, the cultivation of short-cycled crops during the transition season, and combinations of both. Dynamics of soil N_min, nitrate leaching, nitrous oxide emissions, and crop N uptake were studied throughout the year between 2004 and 2005 and partial N balances of the cropping systems were established. In the traditional system (bare fallow between wheat and rice) a large accumulation of soil nitrate N and its subsequent disappearance upon soil saturation occurred during the transition season. This nitrate loss was associated with nitrate leaching (6.3 and 12.8 kg ha⁻¹ at the low and high altitude sites, respectively) and peaks of nitrous oxide emissions (120 and 480 mg m⁻² h⁻¹ at the low and high altitude sites, respectively). Incorporation of wheat straw at 3 Mg ha⁻¹ and/or cultivation of a nitrate catch crop during the transition season significantly reduced the build up of soil nitrate and subsequent N losses at the low altitude site. At the high altitude site, cumulative grain yields increased from 2.35 Mg ha⁻¹ with bare fallow during the transition season to 3.44 Mg ha⁻¹ when wheat straw was incorporated. At the low altitude site, the cumulative yield significantly increased from 2.85 Mg ha⁻¹ (bare fallow) to between 3.63 and 6.63 Mg ha⁻¹, depending on the transition season option applied. Irrespective of the site and the land use option applied during the transition season, systems N balances remained largely negative, ranging from −37 to −84 kg N ha⁻¹. We conclude that despite reduced N losses and increased grain yields the proposed options need to be complemented with additional N inputs to sustain long-term productivity.     

Grain number and grain weight in wheat lines contrasting for stem water soluble carbohydrate concentration

In wheat, the ability to store and remobilise large amounts of stem water soluble carbohydrates (WSC) to grain constitutes a desirable trait to incorporate into germplasm targeted to regions with frequent terminal drought. The main aim of this paper was to examine the relationships between WSC storage, grain number and grain weight across several environments. A small set of recombinant inbred lines (2–4) contrasting in stem WSC were grown in six field trials where water availability, sowing date and/or N level were manipulated, with line yields ranging from 400 to 850 g m⁻² across experiments. Biomass, N and WSC concentration (WSCc, mg g⁻¹ dry weight) and amount (WSCa, g m⁻²) were monitored. A resource-oriented area-based model [Fischer, R.A., 1984. Growth and yield of wheat. In: Smith, W.H., Bante, S.J. (Eds.), Potential Productivity of Field Crops Under Different Environments. International Rice Research Institute, Los Baños, pp. 129–154] and intrinsic rates of organ growth were used to investigate the consequences on grain number of potential competition between spike and stem around flowering.     

Movements by wild brown trout in a boreal river: response to habitat and flow contrasts

Abstract  Radio transmitters were implanted in wild brown trout, Salmo trutta L., in the River Måna at low summer water flows ( n  = 18), higher flow in summer ( n  =   20), and variable, peaking flows in autumn ( n  =   20), and tracked two to four times day and night for 4–5 weeks. Individuals were caught and released in a 4-km uniformly channelised section, and in a 4-km natural diverse river section. Substantial individual variation in home range and total movement (924–85 818 m² and 295–7014 m) suggested flexibility to adapt to local environmental conditions. Fish were stationary most of the time (median movement 0 m), but some individuals undertook few and apparently sporadic longer movements, sometimes involving shifts in home range. No consistent diurnal pattern in movements was found. Trout in the uniform habitat section appeared to have larger home ranges and moved more than trout in the natural section. Differences were, however, not statistically significant in most comparisons, due to large individual variation. Similarly, larger home ranges and movements between trials related to higher flow were found, but differences were generally not significant. No consistent effects of sudden, extreme peaking flows on area use or movements by the brown trout were observed.     

Processes of collating a European fisheries database to meet the objectives of the European Union Water Framework Directive

Abstract  FIsh Database of European Streams, a common database for the FAME project, was merged using existing data on electric fishing and environmental data. FIsh Database of European Stream is a relational database with eight tables. Metrics based on classification of fish species into guilds were calculated, and provided in separate tables. FIsh Database of European Stream contained information about 150 freshwater fish species, from 12 countries, 17 ecoregions, 40 main river regions, 2651 rivers and 8228 sites. Examples of data coverage and use are given. Relationships between environmental variables were illustrated using principal component analysis, which resulted in three environmental components – latitude, size and altitude. Environmental component scores were correlated with fish metrics used in the European Fish Index. Results exemplify how fish guilds reflect gradients in environmental variation. Benefits and problems concerning standardisation and data availability at the global level are discussed.     

Molecular evidence for cosmopolitan distribution of platyhelminth parasites of tunas (Thunnus spp.)

Global distribution of platyhelminth parasites and their host specificities are not well known. Our hypothesis was that platyhelminth parasites of large pelagic fishes are common around the world. We analysed molecular variation in three different taxa of platyhelminth parasites infecting four species of tunas: yellowfin tuna (Thunnus albacares, Scombridae) from Western Australia, southern bluefin tuna (Thunnus maccoyii, Scombridae) from South Australia, Pacific bluefin tuna (Thunnus orientalis, Scombridae) from Pacific Mexico and northern bluefin tuna (T. thynnus, Scombridae) from two localities in the Mediterranean (Spain and Croatia). Comparisons of ITS2 and partial 28S rDNA demonstrated two congeneric species of blood flukes (Digenea: Sanguinicolidae) from multiple hosts and localities: Cardicola forsteri from southern bluefin and northern bluefin tunas, and Cardicola sp. from Pacific bluefin and northern bluefin tunas; and a gill fluke, Hexostoma thynni (Polyopisthocotylea: Hexostomatidae), from yellowfin, southern bluefin and northern bluefin tunas. Partial 28S rDNA indicates that a second type of fluke on the gills, Capsala sp. (Monopisthocotylea: Capsalidae), occurs on both southern bluefin and Pacific bluefin tunas. This appears to be the first report of conspecific platyhelminth parasites of teleosts with a wide‐ranging geographical distribution that has been confirmed through molecular approaches. Given the brevity of the free‐living larval stage of both taxa of flukes on the gills (H. thynni and Capsala sp.), we conclude that the only feasible hypothesis for the cosmopolitan distribution of these flatworms is migrations of host tunas. Host migration also seems likely to be responsible for the widespread occurrence of the two species of blood flukes (Cardicola spp.), although it is also possible that these were translocated recently by the spread of infected intermediate hosts.     

Spatial and non-spatial risk factors associated with cage-level distribution of infectious salmon anaemia at three Atlantic salmon, Salmo salar L., farms in Maine, USA

The distribution of infectious salmon anaemia (ISA) was examined among 80 cages from three Atlantic salmon grow-out farms in Maine, USA that were stocked with smolts from a single hatchery. Cage-level disease was broadly defined as one or more moribund fish testing positive for infectious salmon anaemia virus (ISAV) by RT-PCR and a second confirmatory test (IFAT, culture or genotype sequence). Spatio-temporal and cage-level risks were explored using logistic regression and survival analysis. Non-spatial risk factors associated with ISA, or shortened survival time to disease, included increased predation, trucking company choice for smolt transfers, a finely-sedimented benthic substrate, and smaller average size of smolts at stocking. Univariable analysis identified the time-dependent spatial factor 'adjacency to newly infected cages' to be predictive of new infection in neighbouring cages 11-12 weeks later. However, none of the spatial factors, or their lags retained relevance in multiple-variable models. The results suggest a diffuse distribution of virus exposure throughout infected sites, with host-susceptibility factors probably influencing disease manifestation in individual cages. The narrow focus of the current study may limit application of the findings to other sites and year-classes. However, these data support the relevance of husbandry efforts to optimize fish health in regions affected by ISAV.     

Selected pathological, immunohistochemical and ultrastructural changes associated with an infection by Diphyllobothrium dendriticum (Nitzsch, 1824) (Cestoda) plerocercoids in Coregonus lavaretus (L.) (Coregonidae)

The pathological changes induced by an infection of Diphyllobothrium dendriticum (Nitzsch, 1824) plerocercoids in powan, Coregonus lavaretus (L.), from Loch Lomond, Scotland, were assessed using immunohistochemical and ultrastructural techniques. In a sample of 26 powan, the occurrence of encysted plerocercoids of D. dendriticum on the outer surface of the stomach was 38.5% (n = 10) with the number of cysts ranging from 4 to 15 and measuring 4.2 +/- 1.0 mm x 3.4 +/- 0.9 mm (mean +/- SD). Histological examination of intestinal samples also revealed plerocercoids (2-21) encapsulated within a proliferation of mesenteric fibrous tissues of the gastric wall and, occasionally, by the gut lamina propria-submucosa and lamina muscularis. In section, cysts were tri-layered and were formed from a series of concentric whorls of fibroblast and collagen fibre-based connective elements. The extent of necrosis within each muscle layer and the serosa of the stomach differed, notably within the latter that was marked by a chronic inflammatory reaction and fibrosis. Within the cyst and around it, a large number of degranulating mast cell/eosinophilic granule cells were seen, in addition to melano-macrophage centres. Immunohistochemical staining of sections of infected stomach revealed a high density of elements, in close proximity to plerocercoids, staining positive for serotonin, bombesin, substance P and galanin. Uninfected material did not present the same levels of activity. Sections through both infected and uninfected tissue were also tested for elements containing vasoactive intestinal peptide, met-enkephalin, calcitonin gene-related peptide, neuropeptide Y and nitric oxide synthase, but these were absent.