Effect of canopy cover,seeding depth,and soil moisture on emergence of Centaurea maculosa and C. diffusa*

The effects of canopy cover, seeding depth, and soil moisture on emergence of Centaurea maculosa Lam. and C. diffusa Lam. were studied. Canopy cover had no effect on ermergence rate (seedlings/day/100 seeds) of either species. Seeds of both species emerged faster when placed on the soil surface. Emergence rate decreased as seeding depth increased. Both species required more than 55% initial soil moisture to initiate emergence, with 65–70% being optimum. Percentage emergence followed exactly the same trend as emergence rate. C. maculosa had better emergence characteristics over a wider range of conditions than C. diffusa, possibly being some of the reasons for the former having a wider geographical distribution in the United States and Canada.     

Effects of age on the tryptophan requirement of laying hens

1. White Leghorn pullets which had been used for an assay of tryptophan requirement between 32 and 40 weeks of age were used for similar determinations between 63 and 73 and, after a moult, from 97 to 106 weeks of age.

2. A tryptophan‐limiting protein mixture was used and by dilution seven dietary protein contents were produced, supplying from 0.84 to 1–92 g tryptophan/kg diet. The diet of lowest protein content was also sup‐lemented with free tryptophan. These diets were fed in experiments using 24 groups of 72 pullets at 63 to 73 weeks and 45 groups of 21 hens at 97 to 106 weeks.

3. The relationship between egg output and tryptophan intake was the same in moulted hens as in young pullets, but pullets of 63 to 73 weeks of age yielded a different response curve; more tryptophan being needed for a given egg output.

4. It is concluded that tryptophan required, per day, does not decrease during the first laying year, despite a decrease in rate of egg output.     

Mercury residues in breast muscle of wild ducks, 1970-71.

Samples of breast muscle from 327 ducks collected from October 1970 to March 1971 in the conterminous United States were analyzed for total mercury by flameless atomic absorption spectrometry. Mercury levels for the entire collection ranged from less than 0.01 to 3.91 ppm wet weight with a median of 0.10 ppm. Twenty-five ducks had levels equalling or exceeding the 0.5 ppm guideline for fish and shellfish established by the Food and Drug Administration, U.S. Department of Health, Education, and Welfare. Dabbling ducks, which are shallow-water feeders and mostly vegetarian in fall, winter, and spring, usually had lower levels than diving and sea ducks. Levels were generally higher in ducks collected in areas where environmental mercury levels were known to be greater than in ducks from nonsuspect areas. Despite the mobility of the ducks, levels seemed more closely linked to local environmental contamination than to various factors associated with large geographic areas.     

The effects of ultraviolet-B (UV-B: 290–320 nm) radiation on blister blight disease of tea (Camellia sinensis)

Sunlight is known to reduce the incidence of blister blight disease of tea, caused by Exobasidium vexans . The effects of the ultraviolet-B (UV-B: 290–320 nm) component of solar radiation on the disease and the development of E. vexans were studied in the field in Sri Lanka using UV-screening filter materials held over a commercial crop. Exclusion of UV-B radiation by Polyester, which reduces fluxes in the UV-B region by approximately 75–85%, increased both the number of translucent spots (immature sites of infection) and number of sporulating blisters (mature sites of infection), but it had little or no effect on the sporulation of the pathogen. When basidiospores were artificially inoculated on leaves, and were exposed to full or filtered solar radiation, their survival and germination increased when UV-B wavelengths were removed; significant differences were found 62 h after inoculation. However, UV-B did not affect the extension of germ tubes. This study indicates that the UV-B component of solar radiation plays an important role in the natural regulation of blister blight disease in the field.