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81.
de Bruin T de Rooster H van Bree H Cox E 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2005,52(9):460-465
Proliferation assays performed on peripheral blood mononuclear cells (PBMC) are commonly used in experimental and clinical immunology. A prerequisite for an in vitro assay is the ability to obtain relatively pure populations of mononuclear cells from whole blood, as contaminating polymorphonuclear cells may affect the proliferation of lymphocytes. Purification of canine leucocytes from whole blood is associated with difficulties in obtaining pure lymphocytes in high yields. The aim of this study was to optimize the lymphocyte purification from canine whole blood in terms of total cell recovery and purity, while not influencing the proliferation capacity of the isolated cells. To acquire optimal isolation of canine lymphocytes several density gradient media of different densities and osmolalities were examined. For optimal phagocyte removal, pre-treatment of whole blood with carbonyl iron/arabic gum and/or adherence to fibrinogen pre-coated polystyrene tissue flasks were examined. Lectin-induced proliferation was used as measurement of cell activity of the obtained cell fractions after the different separation procedures. Canine blood pre-treated with carbonyl iron/arabic gum followed by density gradient centrifugation with medium 'G' (density: 1.079 g/cm(3), osmolality: 256 mOsm) and adherence to pre-coated polystyrene tissue flask obtained the best PBMC cultures with a median lymphocyte purity of 88% and a median yield of recovered lymphocytes of 54%. This culture also resulted in the highest proliferation and subsequently the highest stimulation index upon lectin stimulation. 相似文献
82.
Isolation and identification of goose parvovirus in the UK 总被引:2,自引:0,他引:2
83.
84.
Malignant hyperthermia in a Greyhound 总被引:6,自引:0,他引:6
R J Bagshaw R H Cox D H Knight D K Detweiler 《Journal of the American Veterinary Medical Association》1978,172(1):61-62
A rapid rise in rectal temperature (to 45 C) in a young male Greyhound was attributed to the use of halothane and succinylcholine. Circulatory failure and death occurred within approximately 90 minutes of the onset of the hyperthermia. 相似文献
85.
86.
The influence of the level of magnesium (Mg) in the ration on reproduction by the hen was studied in 2 experiments. In experiment 1, it was found that feeding a semi‐purified ration, containing 56 p.p.m. of Mg, resulted in decreased egg production and reduced serum Mg concentration within 10 to 14 days. Although fertility of eggs was not affected noticeably by Mg deficiency, hatchability of fertile eggs was decreased markedly. The influence of inadequate dietary Mg was partially alleviated by adding 250 p.p.m. Mg to the ration. In experiment 2, a decline in hatchability preceded decreases in egg production and food consumption. A decrease in Mg concentration in egg contents accompanied the decline in hatchability. Hens fed rations containing 50 or 150 p.p.m. added Mg produced eggs of relatively low hatchability by day 14 of the trial. Subsequent to being fed a ration containing 550 p.p.m. added Mg, these hens increased in rate of egg production while hatchability of fertile eggs also increased. The results indicate that the dietary Mg requirement of the laying hen, sufficient to maintain a high rate of production of eggs which hatch satisfactorily, is 400 or more p.p.m. 相似文献
87.
88.
89.
The binding kinetics of radiolabeled Salmonella california 1989/O (mannose-sensitive hemagglutinin-positive [MSHA+]) to immobilized mucus or enterocytes isolated from broiler ceca and inhibition of binding by D-mannose and sodium metaperiodate were characteristic of adherence of mannose-sensitive type 1 fimbriae of bacteria to eukaryotic mannose-containing receptors. Binding by radiolabeled strains 1989/O (in the presence of D-mannose) and S. typhimurium S 7471 N (MSHA-, non-fimbriated) indicated non-specific binding that was characterized by less binding to enterocytes and mucus and lack of inhibition by carbohydrates or prior treatment with sodium metaperiodate. Inhibition of non-specific binding to enterocytes by pretreatment with various enzymes or by the presence of tetramethylurea or p-nitrophenol (known to disrupt hydrophobic interactions) indicate involvement of multiple sites and hydrophobic bonding. Strain-specific outer-membrane preparations inhibited non-specific binding to a greater extent than did lipopolysaccharide, Escherichia coli outer-membrane preparations, or bovine serum albumin. 相似文献
90.
Species of Staphylococcus isolated from animal infections 总被引:3,自引:0,他引:3
One hundred randomly selected clinical strains of staphylococci were identified by species using a commercial micromethod system. Eight species of staphylococci were identified. Staphylococcus intermedius was the most frequent (n = 74) species identified and accounted for 70/74 (94.6%) of the coagulase-positive strains and 70/79 (88.6%) of the total isolates from dogs. Other species identified, in order of their frequency, included S. epidermidis (8), S. aureus (7), S. simulans (4), S. sciuri (2), S. xylosus (2), S. hyicus (2) and S. saprophyticus (1). These results show that at least 8 different species of staphylococci can be recovered from animal infections and that coagulase-positive species such as S. intermedius may be more common than S. aureus. The relative significance of these other species in animal infections needs to be assessed. 相似文献