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81.
Bchira Zitoun Virginie Bronzini de Caraffa Jean Giannettini Catherine Breton Ahmed Trigui Jacques Maury Claude Gambotti Brahim Marzouk Liliane Berti 《Scientia Horticulturae》2008
Eighty-four olive accessions obtained from the National Conservatory of Boughrara-Sfax (Tunisia), previously evaluated for morphological traits, were analysed with 47 random amplified polymorphic DNA (RAPD) markers. They were compared with other olive genotypes originated from Eastern or Western Mediterranean. The highest and lowest similarities between genotypes, estimated by simple matching algorithm, were 0.98 and 0.40, respectively. A dendrogram based on Ward's method and a factorial correspondence analysis (FCA) showed that most of Tunisian accessions are closely related to olive genotypes originating from the Eastern Mediterranean and some are clustering with genotypes originated from the Western Mediterranean. These findings suggested multiple and complex origin of Tunisian olive. A comparative study between a previous morphological analysis and current RAPD assay was carried out and discussed. 相似文献
82.
Background
To face climate change and subsequent rainfall instabilities, crop breeding strategies now include root traits phenotyping. Rapid estimation of root traits in controlled conditions can be achieved by using parallel electrical capacitance and its linear correlation with root dry mass. The aim of the present study was to improve robustness and efficiency of methods based on capacitance and other electrical variables, such as serial/parallel resistance, conductance, impedance or reactance. Using different electrode configurations and stem contact electrodes, we have measured the electrical impedance spectra of wheat plants grown in pots filled with three types of soil.Results
For each configuration, parallel capacitance and other linearly independent electrical variables were computed and their quality as root dry mass estimator was evaluated by a ‘sensitivity score’ that we derived from Pearson’s correlation coefficient r and linear regression parameters. The highest sensitivity score was obtained by parallel capacitance at an alternating current frequency of 116 Hz in three-terminal configuration. Using a clamp, instead of a needle, as a stem electrode did not significantly affect the capacitance measurements. Finally, in handheld LCR meter equivalent conditions, capacitance had the highest sensitivity score and determination coefficient (r 2 = 0.52) at 10 kHz frequency.Conclusion
Our benchmarking of linear correlations between different electrical variables and root dry mass enables to determine more coherent practices for ensuring a sensitive and robust root dry mass estimation, including in handheld LCR meter conditions. This would enhance the value of electrical capacitance as a tool for screening crops in relation with root systems in breeding programs.83.
Evaluation of the Ultraviolet Spectrophotometric Method for the Measurement of Total Nitrogen in Water 总被引:1,自引:0,他引:1
Abstract.— A simple, accurate, and environmentally sound procedure based on spectroscopy in the UV range was modified to measure total nitrogen concentrations in both freshwater and brackishwater. Water samples from freshwater fish ponds were analyzed for total nitrogen with the persulfate digestion method followed by analysis of nitrate with the UV method for comparison with the NAS reagent method. Brackishwater samples with salinities of 8 to 10 ppt from shrimp ponds were digested by the persulfate method and analyzed with the UV method. Percentage recovery of nitrogen and also precision were evaluated for the UV method. Nitrate concentrations in the freshwater persulfate digests determined by the UV method were similar to those obtained by the NAS reagent method ( P < 0.05). The slope of the regression line did not differ from 1.0 ( P < 0.05). Precision of both methods was acceptable over the range of nitrogen concentration used, but precision of the UV method tended to be better than that of the NAS method. Spike recovery averaged 97.4% in the NAS reagent method and 107.2% in the UV method. Interference from organic matter was minor in the UV method, because virtually all of the organic matter was oxidized during the digestion. Precision with brackishwater samples was slightly better than the freshwater samples and percentage spike recovery averaged 102.8%. Because spectrophotometers in most laboratories have the capability of reading in the UV range, the UV method is an attractive alternative for total nitrogen analysis. 相似文献
84.
85.
Availability and plant uptake of nutrients were evaluated in three tropical acid soils (Kandiudult) amended with paper pulp and lime under greenhouse conditions. Amendments were applied to attain target pH values of 5.5, 6.0, and 6.5. A control treatment (no paper pulp or lime added) was also included. Rye grass (Lolium perenne L.) as a test plant was grown for three successive cycles of 40 days each. Extractable nutrients and cumulative nutrient uptake were determined. The application of paper pulp or lime resulted in a significant increase in exchangeable Ca and K and a decrease in exchangeable Mg and extractable Fe, Mn, and Zn. Amendment of soils with paper pulp or lime increased plant uptake of Ca and Mg and decreased that of K, Mn, and Zn. Both amendments behaved similarly, but the effect of lime seemed generally greater than that of paper pulp. Paper pulp in tropical acid soils behaved as a liming agent rather than an organic amendment. Similar to lime, amendment of soils with paper pulp resulted in an increase in availability of Ca and Mg and in a decrease in availability of K, Mn, and Zn for plants. Soil extractions appeared to be appropriate for assessing the availability of Ca, Mn, and Zn. Soil pH and effective cation exchange capacity positively influenced the availability of Ca and negatively the availability of Mn and Zn. Thus, the precision of predicting nutrient availability in paper pulp amended tropical acid soils could be improved by including soil pH or effective cation exchange capacity in relevant regression equations. 相似文献
86.
Effect of organic matter concentration on agricultural limestone dissolution in laboratory soil–water systems
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Laboratory soil – water systems in which soil organic matter was increased by 0.0%, 0.5%, 1.0%, 2.0% and 4.0% by adding dry, chopped ryegrass (Lolium perenne) shoots to sandy soil containing 0.06% organic matter. Agricultural limestone was added to the systems, and pH, alkalinity and hardness of the water were monitored for 65 days. The pH, alkalinity and hardness increased with greater soil organic matter concentration. Amounts of carbon dioxide released by microbial respiration increased at the higher soil organic matter concentrations, and this resulted in more rapid and greater solubility of agricultural limestone. The results suggest that greater soil organic matter concentrations in ponds favour the rate and extent of agricultural limestone dissolution. 相似文献
87.
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89.
Fabiana Dal Pozzo Claude Saegerman Etienne Thiry 《Veterinary journal (London, England : 1997)》2009,182(2):142-151
Bluetongue virus (BTV) is an arthropod-borne virus infecting domestic and wild ruminants. Infection in cattle is commonly asymptomatic and characterised by a long viraemia. Associated with the emergence and the recrudescence of BTV serotype 8 (BTV-8) in Northern and Central Europe, remarkable differences have been noticed in the transmission and in the clinical expression of the disease, with cattle showing clinical illness and reproductive disorders such as abortion, stillbirth and fetal abnormalities. Several investigations have already indicated the putative ability of the European BTV-8 strain to cross the bovine placenta and to cause congenital infections. The current epidemiological and pathological findings present an unusual picture of the disease in affected bovines. 相似文献
90.
Inge M. Hanssen Anneleen Paeleman Lieve Wittemans Kris Goen Bart Lievens Claude Bragard Alfons C. R. C. Vanachter Bart P. H. J. Thomma 《European journal of plant pathology / European Foundation for Plant Pathology》2008,121(2):131-146
Over a period of a few years, Pepino mosaic virus (PepMV) has become one of the most important viral diseases in tomato production worldwide. Infection by PepMV can cause
a broad range of symptoms on tomato plants, often leading to significant financial losses. At present, five PepMV genotypes
(EU, LP, CH2, US1 and US2) have been described, three of which (EU, LP and US2) have been reported in Europe. Thus far, no
correlation has been found between different PepMV genotypes and the symptoms expressed in infected plants. In this paper,
the genetic diversity of the PepMV population in Belgian greenhouses is studied and related to symptom development in tomato
crops. A novel assay based on restriction fragment length polymorphism (RFLP) was developed to discriminate between the different
PepMV genotypes. Both RFLP and sequence analysis revealed the occurrence of two genotypes, the EU genotype and the CH2 genotype,
within tomato production in Belgium. Whereas no differences were observed in symptom expression between plants infected by
one of the two genotypes, co-infection with both genotypes resulted in more severe PepMV symptoms. Furthermore, our study
revealed that PepMV recombinants frequently occur in mixed infections under natural conditions. This may possibly result in
the generation of viral variants with increased aggressiveness. 相似文献