全文获取类型
收费全文 | 78787篇 |
免费 | 4246篇 |
国内免费 | 93篇 |
专业分类
林业 | 3185篇 |
农学 | 2042篇 |
基础科学 | 461篇 |
8560篇 | |
综合类 | 15395篇 |
农作物 | 3124篇 |
水产渔业 | 3414篇 |
畜牧兽医 | 41661篇 |
园艺 | 846篇 |
植物保护 | 4438篇 |
出版年
2019年 | 718篇 |
2018年 | 1091篇 |
2017年 | 1211篇 |
2016年 | 1047篇 |
2015年 | 902篇 |
2014年 | 1188篇 |
2013年 | 2618篇 |
2012年 | 2110篇 |
2011年 | 2571篇 |
2010年 | 1615篇 |
2009年 | 1727篇 |
2008年 | 2593篇 |
2007年 | 2403篇 |
2006年 | 2282篇 |
2005年 | 2133篇 |
2004年 | 2076篇 |
2003年 | 2135篇 |
2002年 | 1921篇 |
2001年 | 2384篇 |
2000年 | 2438篇 |
1999年 | 1868篇 |
1998年 | 719篇 |
1997年 | 711篇 |
1996年 | 711篇 |
1995年 | 805篇 |
1993年 | 740篇 |
1992年 | 1553篇 |
1991年 | 1750篇 |
1990年 | 1634篇 |
1989年 | 1646篇 |
1988年 | 1472篇 |
1987年 | 1534篇 |
1986年 | 1596篇 |
1985年 | 1547篇 |
1984年 | 1184篇 |
1983年 | 1057篇 |
1982年 | 704篇 |
1979年 | 1152篇 |
1978年 | 894篇 |
1977年 | 755篇 |
1975年 | 774篇 |
1974年 | 1024篇 |
1973年 | 1024篇 |
1972年 | 1043篇 |
1971年 | 993篇 |
1970年 | 987篇 |
1969年 | 903篇 |
1968年 | 810篇 |
1967年 | 799篇 |
1966年 | 732篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
991.
OBJECTIVE: To determine the effect of 6 plasma ketamine concentrations on the minimum alveolar concentration (MAC) of isoflurane in dogs. ANIMALS: 6 dogs. PROCEDURE: In experiment 1, the MAC of isoflurane was measured in each dog and the pharmacokinetics of ketamine were determined in isoflurane-anesthetized dogs after IV administration of a bolus (3 mg/kg) of ketamine. In experiment 2, the same dogs were anesthetized with isoflurane in oxygen. A target-controlled IV infusion device was used to administer ketamine and to achieve plasma ketamine concentrations of 0.5, 1, 2, 5, 8, and 11 microg/mL by use of parameters obtained from experiment 1. The MAC of isoflurane was determined at each plasma ketamine concentration, and blood samples were collected for ketamine and norketamine concentration determination. RESULTS: Actual mean +/- SD plasma ketamine concentrations were 1.07 +/- 0.42 microg/mL, 1.62 +/- 0.98 microg/mL, 3.32 +/- 0.59 microg/mL, 4.92 +/- 2.64 microg/mL, 13.03 +/- 10.49 microg/mL, and 22.80 +/- 25.56 microg/mL for target plasma concentrations of 0.5, 1, 2, 5, 8, and 11 microg/mL, respectively. At these plasma concentrations, isoflurane MAC was reduced by 10.89% to 39.48%, 26.77% to 43.74%, 25.24% to 84.89%, 44.34% to 78.16%, 69.62% to 92.31%, and 71.97% to 95.42%, respectively. The reduction in isoflurane MAC was significant, and the response had a linear and quadratic component. Salivation, regurgitation, mydriasis, increased body temperature, and spontaneous movements were some of the adverse effects associated with the high plasma ketamine concentrations. CONCLUSIONS AND CLINICAL RELEVANCE: Ketamine appears to have a potential role for balanced anesthesia in dogs. 相似文献
992.
Several hormones regulate Na+, K+-ATPase content in the muscle cell membrane, which is essential for maintaining muscle cell excitability. Chronic glucocorticoid excess is associated with muscle weakness and reduced endurance. We hypothesized that chronic glucocorticoid excess affects Na+, K+-ATPase content in canine skeletal muscle, and contributes to reduced endurance and muscle weakness associated with pituitary-dependent hyperadrenocorticism (PDH) in dogs. Therefore, Na+, K+-ATPase content in skeletal muscle was evaluated before and after hypophysectomy and hormone replacement (cortisone and l-thyroxin) in dogs with PDH (n = 13), and in healthy controls (n = 6). In addition, baseline and exercise-induced changes in plasma electrolyte concentrations and acid–base balance were evaluated before and after hypophysectomy in dogs with PDH. Na+, K+-ATPase content of gluteal muscle in dogs with PDH was significantly lower than in control dogs (201 ± 13 pmol/g versus 260 ± 8 pmol/g wet weight; P < 0.01). Similar differences were found in palatine muscle. After hypophysectomy and on hormone replacement, Na+, K+-ATPase was increased (234 ± 7 pmol/g wet weight). Both plasma pH and base excess in dogs with PDH (7.44 ± 0.01; 1.7 ± 0.6 mmol/l, respectively) were significantly higher (P < 0.05) than after hypophysectomy and hormone replacement (7.41 ± 0.01; −0.2 ± 0.4 mmol/l, respectively). Exercise induced respiratory alkalosis, but did not result in hyperkalemia in dogs with PDH. In conclusion, chronic glucocorticoid excess in dogs with PDH is associated with decreased Na+, K+-ATPase content in skeletal muscle. This may contribute to reduce endurance in canine PDH, although dogs with PDH did not exhibit exercise-induced hyperkalemia. Na+, K+-ATPase content normalized to values statistically not different from healthy controls after hypophysectomy and hormone replacement. 相似文献
993.
The effect of breed, stage of lactation and parity on sheep milk fat CLA content under the same feeding practices 总被引:1,自引:0,他引:1
The cis-9, trans-11 C18:2 (CLA) content in milk fat of four pure sheep breeds (Awassi, Lacaune, Friesland and Chios) was examined. All sheep were kept indoors all year round under the same feeding practices, without any grazing at all. Sheep nutrition was based on alfalfa hay and concentrates. A total of 237 individual milk samples were collected at three sampling times (December, January and March) from sheep of different parity and different days in milk, for CLA determination. The results showed that: (a) there was a large variation in milk fat CLA content among individuals consuming the same diet, (b) the CLA content of milk fat was lower in Friesland breed, compared to the other three breeds but without any significant difference among the four sheep breeds, (c) the CLA content of milk fat was not affected by ewes parity or days in milk, (d) there was a negative but not significant correlation between milk fat and CLA concentration, (e) the CLA content of milk fat was not correlated with milk yield. In conclusion, no others factors, such as breed, parity, days in milk, etc., can affect CLA content in milk fat, which means that the dietary ones remain the sovereign factors explaining the highest proportion of CLA content variability in sheep milk fat. 相似文献
994.
Guo Q Richert BT Burgess JR Webel DM Orr DE Blair M Grant AL Gerrard DE 《Journal of animal science》2006,84(11):3071-3078
Feeding increased levels of dietary vitamin E can inhibit lipid oxidation. The aim of this study was to investigate the effect of levels of dietary alpha-tocopherol acetate (VE) and feeding duration on meat quality and lipid oxidation. Eighty-one pigs were allocated to 1 of 3 diets containing 40, 200, or 400 IU of VE/kg of feed, and each diet group was divided into 3 feeding periods (3, 6, or 9 wk). Carcass characteristics and meat quality were evaluated. Oxidative stability of fresh and cooked pork patties and pork chops was determined after chilled or frozen storage. Increasing dietary concentrations of VE did not affect any growth performance parameter. Drip loss, however, decreased (P < 0.05) with increased dietary VE levels. Moreover, an increased duration of VE feeding improved (P < 0.05) pH and drip loss. Less lipid oxidation (P < 0.05) was detected in fresh ground pork from pigs fed greater concentrations of VE after 4 d of storage. A greater (P < 0.05) resistance to oxidation in cooked ground pork was observed in pigs fed 200 or 400 IU of VE/kg at 2 and 6 d of storage. Fresh and cooked pork patty oxidation decreased (P < 0.05) linearly as feeding duration increased from 3 to 9 wk. After 6 mo of freezer storage, lipid oxidation of pork chops from pigs fed 200 or 400 IU of VE/kg was lower (P < 0.05) than for pigs fed 40 IU of VE/kg. Likewise, lipid oxidation of pork chops of pigs fed VE for an extended period of time (6 wk) was lower (P < 0.05) after 9 mo of storage. Fatty acid profiles of neutral lipid fraction of the LM became more unsaturated (P < 0.05) with added VE to the feed. These results indicate an increased intake of dietary VE concentration, and prolonged feeding of VE can improve drip loss and reduce lipid oxidation in ground pork and pork chops. This study suggests that supplementation with 200 IU of VE/kg of feed for 6 wk before market is beneficial in improving lipid stability and pork quality. 相似文献
995.
996.
Brownlie J Nuttall PA Stott EJ Taylor G Thomas LH 《Veterinary immunology and immunopathology》1980,1(4):371-378
Certain immunological responses of 4-6 month old calves experimentally inoculated with either cytopathic or non-cytopathic bovine virus diarrhoea virus (BVDV) were compared with those of uninfected control calves. The tests used to demonstrate the immunological responses were the transformation of lymphocytes by PHA mitogen, the percentage of lymphocytes with surface immunoglobulin, and the antibody titres induced by an intravenous inoculation of killed Brucella abortus. There were no significant differences between the two groups of calves and therefore, the mild experimental disease produced by BVDV did not appear to affect adversely the immunological response. 相似文献
997.
998.
Anal furunculosis: a review of seventy cases 总被引:1,自引:0,他引:1
J. E. F. HOULTON 《The Journal of small animal practice》1980,21(11):575-584
The response to treatment of seventy cases of anal furunculosis is reviewed. A comparison is made between a surgical technique employing cutting diathermy and a cryosurgical procedure. The reasons for the poor results obtained with each method are discussed. 相似文献
999.
A storage-like disease in a 14-month-old Rhodesian Ridgeback dog is described. Clinically the dog was lame on all legs, and appendicular joint capsules were distended. Radiographically and at necropsy, there was evidence of osteoarthritis, increased volume of synovial fluid, and marked hypertrophy of the synovial membranes. Large mononclear cells with vacuolated cytoplasm were evident in synovial membranes. Large mononuclear cells with vacuolated cytoplasm were evident in synovial membranes. Large mononuclear cells with vacuolated cytoplasm were evident in synovial membranes, bone marrow, liver, lymph nodes, and endocrine glands. Vacuolated cells in lymph nodes stained positively for fat. Transmission electron microscopy revealed empty membrane-bound cytoplasmic vacuoles. The similarity of this disease to storage diseases in man where arthropathy is a feature is discussed. 相似文献
1000.
Marion D. Francis Candice L. Slough Hugh E. Black Andrew J. Tofe G. Gilbert Cloyd 《Veterinary radiology & ultrasound》1980,21(4):168-176
The following sequence of treatments was administered to a Saint Bernard dog with a primary distal right radius osteosarcoma: 54 days of daily disodium 1-hydroxyethylidenediphosphonate (HEDP) subcutaneous injections; 53 days of HEDP per os ; one 32 P-HEDP intravenous injection. During the pretreatment period, there was an extensive increase in calcific tumor growth and osteoblastic proliferation. After the subcutaneous HEDP treatment, almost complete tumor necrosis was seen. After the oral HEDP treatment, only the deepest tumor portion contained active osteoblasts, calcific growth of the tumor was completely blocked, and uptake of 99m Tc-Sn-HEDP was reduced to one fourth of the pretreatment uptake. After a single 32 P-HEDP dose, large areas of tumor necrosis were evident histopathologically. However, subsequent resumption of cellular activity occurred in the tumor, and the uptake of 99m Tc-Sn-HEDP increased to pretreatment values. These data suggest that systemically administered HEDP should be studied further for its possible therapeutic potential in the treatment of osteosarcoma and indicate a need for further study of 32 P-HEDP or possibly 33 P-HEDP. 相似文献