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921.
The origin and mechanisms of human interictal epileptic discharges remain unclear. Here, we describe a spontaneous, rhythmic activity initiated in the subiculum of slices from patients with temporal lobe epilepsy. Synchronous events were similar to interictal discharges of patient electroencephalograms. They were suppressed by antagonists of either glutamatergic or gamma-aminobutyric acid (GABA)-ergic signaling. The network of neurons discharging during population events comprises both subicular interneurons and a subgroup of pyramidal cells. In these pyramidal cells, GABAergic synaptic events reversed at depolarized potentials. Depolarizing GABAergic responses in neurons downstream to the sclerotic CA1 region contribute to human interictal activity. 相似文献
922.
923.
924.
Wörner HJ Bertrand JB Fabre B Higuet J Ruf H Dubrouil A Patchkovskii S Spanner M Mairesse Y Blanchet V Mével E Constant E Corkum PB Villeneuve DM 《Science (New York, N.Y.)》2011,334(6053):208-212
Conical intersections play a crucial role in the chemistry of most polyatomic molecules, ranging from the simplest bimolecular reactions to the photostability of DNA. The real-time study of the associated electronic dynamics poses a major challenge to the latest techniques of ultrafast measurement. We show that high-harmonic spectroscopy reveals oscillations in the electronic character that occur in nitrogen dioxide when a photoexcited wave packet crosses a conical intersection. At longer delays, we observe the onset of statistical dissociation dynamics. The present results demonstrate that high-harmonic spectroscopy could become a powerful tool to highlight electronic dynamics occurring along nonadiabatic chemical reaction pathways. 相似文献
925.
A (4.60+/-0.07)x10(9) year internal isochron has been drawn for the achondrite Juvinas by the rubidium-87/strontium-87 method. Earlier petrographic investigation of achondrites supplemented by a new ion microprobe study of Juvinas strongly suggest an igneous origin for this class of meteorites. The results thus indicate that igneous activity may have rapidly followed the formation of the achondrites' parent body 4.6x10(9) years ago. 相似文献
926.
Forest cover reduction may affect movements of forest animals, but resistance to animal movements in and out of forests remains
unknown despite its importance for modelling. We tested whether ovenbird (Seiurus aurocapilla), a forest-interior songbird, responds similarly to the amount of forest cover while moving locally (~2 km) and over entire
landscapes (~25 km). We compared spatially-explicit simulations to field data to address the issue of resistance to movement
in open areas. We caught, banded and translocated 143 territorial males 0.8–27 km away from their territory early in the breeding
season. Seventy-eight percent and 50% of translocated males returned (homed) within 10 days following “local” and “landscape”
translocations respectively. Independent of translocation distance, homing times increased with decreasing forest in the landscape.
With a Geographic Information System (GIS), we simulated “least-cost” paths that homing ovenbirds would ideally take, when
resistance to movement in open areas ranged 1–1000 times the resistance to movement in forest. The length, the cumulative
cost, and variability of simulated least-cost movement paths increased with increasing resistance in open areas. With landscape
translocations, least-cost path length explained homing time better than Euclidean distance, and based on an information-theoretic
approach, resistance to movement was estimated to be 27 times greater in open areas than in forests (95% confidence interval:
16–45). However, least-cost path length did not perform better than Euclidean distance with local translocations, and the
cumulative cost of least-cost paths was not associated to homing time in either translocation scale. We conclude that resistance
to animal movements in open areas can be addressed by a combination of GIS modelling and translocation experiments, and is
between one and two orders of magnitude greater than resistance to movements in forests, in the case of ovenbirds. 相似文献
927.
Conservation strategies should be based on a solid understanding of processes underlying species response to landscape change.
In forests fragmented by agriculture, elevated nest predation rates have been reported in many forest bird species, especially
near edges. In intensively-managed forest landscapes, timber harvesting might also be associated with negative edge effects
or broader “context” effects on some species when the matrix provides additional resources to their major nest predators.
In this study, we hypothesized that proximity to a forest edge and proportion of cone-producing plantations will increase
nest predation risk in fragments of relatively undisturbed forest. We focused on the Brown Creeper (Certhia americana), an indicator species of late-seral forests. We compared habitat configuration and composition at four spatial scales (0.14,
0.5, 1 and 2 km) around 54 nests and related daily nest survival rate to the distance to the nearest forest edge, mean patch
size of late-seral forest (r = 141 m), proportion of non-forested lands (r = 141 m), density of maintained roads (r = 1 km), proportion of cone-producing spruce plantations (r = 2 km), and year. The best model included distance to the nearest edge and proportion of cone-producing plantations. Distance
of nests to the nearest edge was the best individual predictor of daily nest survival. A larger sample of nests showed a significant
threshold in distance to the nearest forest edge; nests located at least 100 m away were more likely to fledge young. These
results suggest that even in managed forest landscapes, matrix effects can be important and some bird species may exhibit
negative edge effects. 相似文献
928.
Sampedro I Aranda E Rodríguez-Gutiérrez G Lama-Muñoz A Ocampo JA Fernández-Bolaños J García-Romera I 《Journal of agricultural and food chemistry》2011,59(7):3239-3245
Byproducts generated from food industries, such as olive oil mills, have been studied to decrease harmful pollution and their environmental consequences. In this work, a new thermal pretreatment and saprobic fungal incubation to detoxify alperujo (two-phase olive mill waste) have been evaluated in view of its use as fertilizer in agriculture. The sequential use of both methods simplifies the thermal conditions and incubation times of the fungal treatment. Optimization of the thermal treatment from 150 to 170 °C for 45 and 15 min, respectively, reduced the incubation time with Coriolpsis rigida from 20 to 10 weeks needed to reduce phytotoxic effects on tomato plants. Therefore, the combination of thermal and biological treatments will allow the development of the potential benefits of alperujo to improve nutrients in agricultural soil. 相似文献
929.
Moretton C Crétier G Nigay H Rocca JL 《Journal of agricultural and food chemistry》2011,59(8):3544-3550
4-Methylimidazole (4MeI) is a nitrogen compound formed during the manufacture of class III and IV caramel colors. The European Commission has limited its content to 250 ppm. Two methods were compared to perform 4MeI quantification in caramels. The first one, currently used and considered to be the reference method, consists of a hot extraction of caramel color with dichloromethane and an analysis of the acetyl derivative of the extract by gas chromatography coupled to mass spectrometry (GC-MS). The second method is based on the heart-cutting two-dimensional liquid chromatography technique (LC-LC) to directly separate 4MeI from the other components present in caramel color sample (diluted in water) in <30 min. The accuracy profile validation method and the comparison between the results obtained with the two methods show that the new and completely automated LC-LC method is usable to quantify 4MeI in caramels. 相似文献
930.
Cakir-Kiefer C Le Roux Y Balandras F Trabalon M Dary A Laurent F Gaillard JL Miclo L 《Journal of agricultural and food chemistry》2011,59(9):4464-4472
α-Casozepine is a peptide, corresponding to the sequence 91-100 of the bovine α(s1)-casein, displaying anxiolytic activity in the rat. The α(s1)-casein tryptic hydrolysate containing this peptide decreases stress effects after oral administration in various species including man. Therefore, the stability of this peptide toward gastric and pancreatic proteases has been assessed by using pepsin, chymotrypsin/trypsin, Corolase PP, pepsin followed by chymotrypsin/trypsin or pepsin followed by Corolase PP. α-Casozepine was slowly degraded by chymotrypsin, much more sensitive to pepsin and Corolase PP but not completely destroyed after 4 h kinetics. The bonds in the region 91 to 95 of the α-casozepine were totally resistant to hydrolysis by all studied proteases. Surprisingly, a fragment, corresponding to the sequence 91-97 and found in all the hydrolysis media in significant amount, possessed an anxiolytic activity in three behavioral tests measuring this parameter. This peptide could participate in the in vivo activity of α-casozepine. 相似文献