Clinical and echocardiographic features of mild mitral valve regurgitation in 108 horses

The clinical and echocardiographic characteristics of 108 horses with echocardiographically confirmed mild mitral valve regurgitation (MR) were investigated along with its clinical progression. Follow-up consisted of a re-examination of 28 horses and questionnaires were used to obtain information on a further 43 cases. Thirty-seven horses with mild MR were lost to follow-up. Horses with mild MR were re-examined between 2 and 9 years (3.8 ± 1.8 years) following first presentation, with mild MR still present and a small, but statistically significant (P = 0.049) increase of left ventricular diameter in end-diastole. These results suggested that mild MR has a good mid-term prognosis in sport and pleasure horses.     

Ecometrics: The traits that bind the past and present together

We outline here an approach for understanding the biology of climate change, one that integrates data at multiple spatial and temporal scales. Taxon-free trait analysis, or “ecometrics,” is based on the idea that the distribution in a community of ecomorphological traits such as tooth structure, limb proportions, body mass, leaf shape, incubation temperature, claw shape, any aspect of anatomy or physiology can be measured across some subset of the organisms in a community. Regardless of temporal or spatial scale, traits are the means by which organisms interact with their environment, biotic and abiotic. Ecometrics measures these interactions by focusing on traits which are easily measurable, whose structure is closely related to their function, and whose function interacts directly with local environment. Ecometric trait distributions are thus a comparatively universal metric for exploring systems dynamics at all scales. The main challenge now is to move beyond investigating how future climate change will affect the distribution of organisms and how it will impact ecosystem services and to shift the perspective to ask how biotic systems interact with changing climate in general, and how climate change affects the interactions within and between the components of the whole biotic-physical system. We believe that it is possible to provide believable, quantitative answers to these questions. Because of this we have initiated an IUBS program iCCB (integrative Climate Change Biology).     

Search of environmental descriptors to explain the variability of the bacterial diversity from maize rhizospheres across a regional scale

The regional scale variability of the bacterial community inhabiting the rhizosphere was studied with soil collected from maize fields located in the Santo Domingo Valley (SDV; Baja California Sur, Mexico), a semi-arid agricultural ecosystem of approximately 200 km². The bacterial community structure was visualized by single-strand conformation polymorphism (SSCP) profiles of PCR-amplified partial 16S rRNA genes of directly extracted rhizosphere soil DNA. SSCP profiles of different SDV sites and an external field site in Germany were evaluated for their similarities and the contributing bacteria were characterized by DNA sequence analyses. SSCP profiles from each site were significantly different from the others, as revealed by permutation of pairwise similarities (P < 0.05). In comparison to the German site, SSCP profiles from SDV were more similar to each other despite contrasting soil salinity levels. Correspondence analysis revealed that among SDV sites, salinity levels, soil organic carbon and calcium (Ca²⁺) were most influential on the bacterial community structure. Depending on the phylogenetic group analyzed (Bacteria, Alphaproteobacteria, Pseudomonas), the importance of these soil variables varied. Interestingly, the East–West direction also revealed an effect, suggesting that future explorations of bacterial diversity patterns should also consider landscape topography in search of explaining patterns of bacterial diversity in soils.     

Elevated CO2 stimulates N2O emissions in permanent grassland

To evaluate climate forcing under increasing atmospheric CO₂ concentrations, feedback effects on greenhouse gases such as nitrous oxide (N₂O) with a high global warming potential should be taken into account. This requires long-term N₂O flux measurements because responses to elevated CO₂ may vary throughout annual courses. Here, we present an almost 9 year long continuous N₂O flux data set from a free air carbon dioxide enrichment (FACE) study on an old, N-limited temperate grassland. Prior to the FACE start, N₂O emissions were not different between plots that were later under ambient (A) and elevated (E) CO₂ treatments, respectively. However, over the entire experimental period (May 1998–December 2006), N₂O emissions more than doubled under elevated CO₂ (0.90 vs. 2.07 kg N₂O-N ha⁻¹ y⁻¹ under A and E, respectively). The strongest stimulation occurred during vegetative growth periods in the summer when soil mineral N concentrations were low. This was surprising because based on literature we had expected the highest stimulation of N₂O emissions due to elevated CO₂ when mineral N concentrations were above background values (e.g. shortly after N application in spring). N₂O emissions under elevated CO₂ were moderately stimulated during late autumn–winter, including freeze–thaw cycles which occurred in the 8th winter of the experiment. Averaged over the entire experiment, the additional N₂O emissions caused by elevated CO₂ equaled 4738 kg CO₂-equivalents ha⁻¹, corresponding to more than half a ton (546 kg) of CO₂ ha⁻¹ which has to be sequestered annually to balance the CO₂-induced N₂O emissions. Without a concomitant increase in C sequestration under rising atmospheric CO₂ concentrations, temperate grasslands may be converted into greenhouse gas sources by a positive feedback on N₂O emissions. Our results underline the need to include continuous N₂O flux measurements in ecosystem-scale CO₂ enrichment experiments.     

Effects of experimental soil frost on the fine‐root system of mature Norway spruce

Soil‐frost events may influence the dynamics of fine roots and therefore affect root‐derived C fluxes to the soil. We studied the impact of soil frost on the fine‐root dynamics of Norway spruce (Picea abies [L.] Karst.) by a replicated snow‐removal experiment in a mature forest in SE Germany. Snow removal in the three treatment plots reduced soil temperature significantly with minima <–5.5°C in the O layer while the snow‐covered control plots never reached temperatures below the freezing point. Sequential soil coring in the O layer at the beginning and at the end of the soil‐frost period as well as after thawing revealed that the soil frost treatment increased fine‐root mortality by 29%. However, enhanced fine‐root production in the snow‐removal plots nearly compensated for the fine‐root losses caused by low temperatures. These findings were confirmed by minirhizotron observations in the O layer and the upper 25 cm of the mineral soil showing that relative fine‐root loss was by far higher in the snow‐removal plots than in the control plots. Compensatory fine‐root production in the snow‐removal plots exceeded fine‐root production in the control plots during a period of 8 weeks after the soil frost application by 39% in the O layer while it was similar in both plot types in the mineral soil. Sequential coring and minirhizotron observations led to substantially different fine root–longevity estimates for the soil frost period. However, in both cases, the snow‐removal treatment was characterized by a significant reduction in root longevity indicating a faster fine‐root turnover. As a consequence, experimental soil frost enhanced the C input to the soil via root death at our study site by approx. 42 g m^–2 and stimulated the C investment towards the root system of the spruce trees due to a higher sink activity.     

Recommended urban storm water infiltration devices for different types of run-off under varying hydrogeological conditions

Background, aim, and scope  The decentralised near-natural infiltration of storm water in urban areas over a long-term period can cause local pollution of soil, seepage water and groundwater due to heavy metals (e.g. Pb, Zn, Cu), polycyclic aromatic hydrocarbons (PAH), petroleum hydrocarbons and readily soluble salts, which compounds are partly classified as hazardous. The aim of this paper is to present a recommendation matrix for suitable storm water infiltration devices. The scope is limited to eight different run-off types and two different infiltration devices (swales and trenches with three different trench-filling materials) under two different hydrogeological conditions (high adsorbing soil with low permeability, low adsorbing soil with high permeability). The examined run-off types are sub-divided as follows: run-off from unpaved areas (gardens, grassed areas, cultivated land); green roofs, aluminium roofs; roofs without zinc gutters and down-pipes; roofs with zinc gutters and down-pipes; copper roofs; zinc roofs and trafficked areas (cycle and pedestrian ways, yards, car parks and residential roads). The recommendation matrix should assist decision-makers such as city planners, architects and private house builders. Materials and methods  The potential for storm water infiltration to pollute soil, seepage water and groundwater is investigated with long-term 3-D numerical water flow and chemical transport modelling in unsaturated and saturated zones over 50 years, which were already presented by Zimmermann et al. (Water Sci Technol 51(2):11–19, 2005). The recommendation is based on a comparison between modelling results and several guideline values prepared by several German authorities. The evaluation process leads to four hazard levels regarding the impact on topsoil (i.e. first 20 cm of the soil), on seepage water (1 m below the infiltration device) and on groundwater (at the unsaturated–saturated boundary). Results  The recommendation matrix consists of 56 individual statements. Relating to dissolved organic substances like phenanthrene and fluoranthene, the infiltration of trafficked areas run-off is critical. The infiltration of metalliferous run-off has a high hazard accumulation potential. Here the storm water infiltration via sub-ground of low permeability and high adsorbing soil material is critical for seepage water in any case; the infiltration of zinc roofs run-off via trench infiltration devices is even critical for groundwater at 4 m depth. Sub-ground of low permeability and high adsorbing soil material has a lower potential hazard in terms of storm water infiltration from roof run-off. The storm water infiltration via swales effects a very large accumulation of heavy metals in the topsoil. The storm water infiltration via trenches leads to the accumulation of hazardous substances in the deeper sub-ground, particularly where the trench-filling material has low adsorbing capacity and high permeability. Discussion  The transferability of the results to other sites depends particularly on the hydrogeological conditions. Before using the recommendation matrix, details of the hydrogeological conditions should be collected. The long-term simulation process is simplified by several impact factors such as non-constant rainfall, soils heterogeneity, macro-porous flow, particle-bounded transport and microbiological decomposition. Conclusions  Based on the scale of risks to soils, seepage water and groundwater, the matrix should be used in the selection of the roof construction materials and appropriate storm water infiltration devices so that the environmental risks can be minimised. If the sub-ground has a high permeability and low adsorption capacities, the infiltration of metalliferous roof run-off water is, in general, not advisable without putting treatment facilities in place upstream. Thus, architects need to realise that the choice of a suitable infiltration device depends, on the one hand, on the type of run-off and, on the other hand, on the hydrogeological condition and the building materials. Recommendations and perspectives  Replacement of the topsoil in swale infiltration devices is recommended because, in particular, heavy metal (zinc) in run-off from roofs with zinc gutters and down-pipes accumulates in the soil matrix. The replacement interval depends on the hydrogeological conditions and, for this run-off example, lies between 10 and 20 years. If infiltration is essential, constructing special treatment facilities upstream can be an alternative. The existing numerical model could be adapted to suit other site-specific materials and be enhanced regarding several complex impact factors.     

Developing a taper model for the Pinus elliottii × P. caribaea var. hondurensis hybrid in South Africa

A relatively new hybrid, Pinus elliottii × P. caribaea var. hondurensis (Pexc), for which a taper model does not exist, is being planted commercially in South Africa. This study primarily focused on developing a taper model for Pexc in South Africa. Taper data were collected from a total of 363 trees, in the Mpumalanga and Limpopo provinces, using a random sampling method. A subsample was selected to determine if altitude, rainfall, temperature or soil have a significant influence on the taper of Pexc. Only rainfall significantly influenced the overall taper. The Max and Burkhart segmented polynomial taper model, as well as the Kozak88, Kozak01 and Kozak02 variable exponent taper models, were fitted, compared and tested using the statistical analysis system (SAS). The predictive ability of the models was evaluated based on the results from the mean bias, standard deviation, the standard error of prediction and the average percentage deviation. The Kozak02 model had the best fit overall followed by the Max and Burkhart model (MB76). The MB76 model, however, predicted the volumes more accurately than the Kozak02 model.     

REMI (Restriction Enzyme Mediated Integration) and its Impact on the Isolation of Pathogenicity Genes in Fungi Attacking Plants

Development of molecular techniques for phytopathogenic fungi aims at the identification of fungal genes whose products are essential for successful infection of the host plant. Initial approaches have relied on isolating candidate genes and generating null-mutations by homologous recombination. Unfortunately, the results of this strategy have not been overly successful. This has led to a search for alternatives which allow an unbiased identification of pathogenicity genes. One method, which has proved successful in several systems, is a tagging mutagenesis procedure termed restriction enzyme mediated integration (REMI). In this mini-review we describe this procedure and review its features and results of its use when applied to the identification of fungal genes required for disease development in planta.     

Niche‐based species distribution models and conservation planning for endangered freshwater crayfish in south‐western Germany

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