Assessment of tillage strategies to decrease nitrate leaching in the Brimstone Farm Experiment, Oxfordshire, UK

Tillage may influence nitrate losses from agricultural soils. Losses of nitrate were measured in drainflow at 60 cm depth and in combined surface runoff and interflow in the A horizon (=surface layer flow) on hydrologically sealed plots with a two-year comparison (1988–1990) of shallow-tine cultivation vs. mouldboard ploughing. Ploughing increased concentrations and loadings of nitrate in drainflow and surface layer flow, especially in the first year. After these two years the shallow-tined plots were ploughed to plant winter beans (Vicia faba L.), and nitrate in drainflow then increased over the next three winters, slightly exceeding that from the plots which had been ploughed throughout for winter cereals. The composition of the surface layer flow did not show this effect, however. Calculations of net winter mineralisation of soil organic nitrogen showed that shallow-tine cultivation may have decreased mineralisation slightly compared with ploughing in the first two years. These calculations did not indicate any increase in mineralisation for two winters after the minimally cultivated plots were ploughed in autumn 1990, probably because the soil was then very dry. This increase was apparently delayed until the fifth winter (1992/1993), which was much wetter than any since autumn 1990. In the previous eight years (1980–1988) half of the plots had been ploughed and half had been direct drilled. Averaged over the five winters 1988/1989–1992/1993, the five measures of nitrate loss in drainflow from plots previously direct drilled were 6–57% more than from plots previously ploughed, and winter mineralisation was 20% more, with no evidence of any decline in either with time. The nitrate produced by mineralisation of organic matter conserved by the eight years of direct drilling was mainly lost by leaching or denitrification; it was of little or no benefit to the crops. The results suggest that in the long term more nitrate is leached from land subject to periods of minimal or zero tillage and ploughing than from land ploughed every year.     

A pericyte origin of spinal cord scar tissue

There is limited regeneration of lost tissue after central nervous system injury, and the lesion is sealed with a scar. The role of the scar, which often is referred to as the glial scar because of its abundance of astrocytes, is complex and has been discussed for more than a century. Here we show that a specific pericyte subtype gives rise to scar-forming stromal cells, which outnumber astrocytes, in the injured spinal cord. Blocking the generation of progeny by this pericyte subtype results in failure to seal the injured tissue. The formation of connective tissue is common to many injuries and pathologies, and here we demonstrate a cellular origin of fibrosis.     

Beyond electronic feeding: The implementation of call feeding for pregnant sows

Call feeding for pregnant sows is a novel modular extension of a conventional electronic feeder (PigTek INTEC MAC) communicating via ISOagriNET. The call feeding module (CFM) assigns individual calls to each animal of a group supplied by one feeder and trains them to associate that call with feed access. Afterwards it actively calls sows to the feeder in a variable sequence in order to minimize queuing and thereby reducing aggression, stress and injuries associated with feeding. In this paper, we describe the automatic training procedures, the principal technical design and implementation details that make call feeding applicable in practice. The automatic training consists of an initial Pavlovian conditioning during standard electronic feeding and a subsequent operant conditioning. During Pavlovian conditioning the sows may enter the feeder whenever they have remaining feed allowance. An individual acoustic signal is then played immediately before the feed is dispensed. In the operant training the sow learns by experience that she can enter the feeder only after the individual acoustic signal has been presented. The training modes and their durations are individual to each sow’s learning success. Undersupply with feed due to incomplete learning is prevented by the training routines without manual intervention by controlling automatically the proportion of operant and Pavlovian conditioning. Thus, introducing call feeding in an existing herd is possible. The implementation is further able to estimate roughly the social hierarchy and incorporates this in the calculation of the call sequence in order to attenuate feeding associated aggressions. It helps to provide the animals with a positive anticipation of safe feeding and thereby offers a suitable way to improve welfare and health of pregnant sows. Being automatically controlled, it is easy to apply and has the potential to become a promising future element of precision livestock farming.     

Structure of fungal fatty acid synthase and implications for iterative substrate shuttling

We report crystal structures of the 2.6-megadalton alpha6beta6 heterododecameric fatty acid synthase from Thermomyces lanuginosus at 3.1 angstrom resolution. The alpha and beta polypeptide chains form the six catalytic domains required for fatty acid synthesis and numerous expansion segments responsible for extensive intersubunit connections. Detailed views of all active sites provide insights into substrate specificities and catalytic mechanisms and reveal their unique characteristics, which are due to the integration into the multienzyme. The mode of acyl carrier protein attachment in the reaction chamber, together with the spatial distribution of active sites, suggests that iterative substrate shuttling is achieved by a relatively restricted circular motion of the carrier domain in the multifunctional enzyme.     

Einfluss von wasser‐ und nährstoffversorgung auf die anfangsentwicklung einer nachsaat

In einem Gefäßversuch wurde der Einfluss der Wasser‐ (mangelhaft: 1 mm d^?1 durch gießen; ausreichend: kapillarer Wasseraufstieg aus 35 cm Bodentiefe) und Nährstoffversorgung (NPK‐Düngung breitwürfig oder Banddtlngung der Nachsaatreihe) auf die Jugendentwicklung einer Nachsaat der Grasart Festucapratensis Huds. untersucht. Die Anzahl nachgesäter Pflanzen erreichte am Ende der Versuchsperioden bei mangelhafter Wasserversorgung 50% bzw. 0% der Pflanzenanzahl der Prüfglieder mit ausreichender Wasserversorgung. Die Banddüngung führte offenbar nicht zu einer verbesserten Nährstoffversorgung und konnte die Etablierung nicht verbessern. Die Ergebnisse deuten darauf hin, dass zunehmende Anteile von Elymus repens L. die Etablierung einer Nachsaat erschweren. Da für die nachgesäten Gräser ein wesentlicher Lichtmangel ausgeschlossen werden kann, scheint die geringe Etablierung der Nachsaat durch Wirkungen im Wurzelraum bedingt zu sein.     

Die Pflanzhaue

Ohne Zusammenfassung     

The effect of different biochars on the growth and water use efficiency of fenugreek (Trigonella foenum-graecum L.)

In two identical pot trials, we investigated the effect of different biochars on the growth and water use efficiency (WUE) of fenugreek (Trigonella foenum-graecum L.) grown with and without water stress. Plants were grown in pots with and without biochar. Rice husk (RH), standard rice husk (SRH), standard wheat straw (SWS) or standard oilseed rape straw (SOS) biochars were added at a rate of 2% by weight. Irrigation was reduced from 80% of field capacity to 40% in half of the pots to create water stress when 50% of the plants flowered. No significant interaction was observed between irrigation and biochar treatments. Water stress resulted in smaller plants (11.5%), fewer pods (16.1%) and reduced 100-seed weight (12.6%), seed yield (20.1%) and plant dry weight (55.0%). All standard biochars increased plant dry weight in a range of 18%−25% compared with the controls. There was no significant effect of biochar on the number of pods and seed weight. Only RH, SWS and SOS biochar increased the number of seeds pod⁻¹ by 9.0%−14.5% compared with the controls regardless of water stress. RH and SOS biochars increased seed yield by 16.7 and 21.6%, respectively. RH, SWS and SOS biochar increased the WUE by 17.3%, 10.1% and 16.2% regardless of water stress, respectively. Based on the experiment, we recommend farmers to add biochar whatever type they have available. However, RH and SOS seem to be better than the others are, as they seem to be able to increase seed yield and WUE considerably.     

Physiological response of ‘Larry Ann’ plums to cold storage and 1-MCP treatment

The aim of this work was to study the specific effects of low temperature and 1-MCP treatment on ethylene metabolism and oxidative behaviour in plums (Prunus × salicina cv. Larry Ann). Control fruit were stored at 20 °C or 0 °C and the 1-MCP (625 nL L^?1) treated fruit at 0 °C. Changes in the kinetics of ethylene production upon removal were related to changes in ACC metabolism (ACC and MACC levels), oxidative behaviour (H₂O₂ content) and enzymatic antioxidant potential (SOD, CAT and POX enzymes) during cold storage. Low temperature stress inhibited the synthesis of MACC, which appeared to be the basic process that regulated ACC and ethylene production at ambient temperature. Although 1-MCP treatment inhibited ethylene production and ACC accumulation in the cold, it did not inhibit the accumulation of MACC. Neither cold nor 1-MCP treatment induced oxidative stress. Nevertheless, the 1-MCP treatment significantly impaired the increase in POX activity observed during cold storage. Collectively these results showed the underlying role that ACC metabolism plays in the ripening behaviour of cold-stored plums, confirming previous results. The results also indicate that MACC and malonyl transferase activity are the key regulatory factors that control ripening and possibly some ethylene-related disorders such as chilling injury in cold-stored plums.     

A web-based ToolBox approach to support adaptive forest management under climate change

The design and implementation of the adaptive forest management (AFM) ToolBox is presented. Design principles derived from previous experiences in decision support system (DSS) development include support for (1) modularity, (2) accessibility via the Internet, (3) inclusion of different types of knowledge and information, (4) the use of different data sources, and (5) specific problem types. As major components of the AFM ToolBox DataBase, Vulnerability Assessment Tools (single user version, group mode) and an optimization tool to generate optimized management plans at the level of management units or landscapes are highlighted. A key feature is the distinction of two archetypical user profiles (manager, analyst). The AFM ToolBox is evaluated against eight criteria for the assessment of DSS. It is concluded that the ToolBox approach setting focus on modularity while avoiding to over-emphasis technical integration provides the right frame to secure the flexibility regarding tools and decision-making processes which is mandatory if a DSS should be taken up by practice.