Application of a tandem mass spectrometer and core-shell particle column for the determination of 151 pesticides in grains

A comparison of ultrahigh performance liquid chromatography (UHPLC) with a 2.6 μm core-shell particle column (Kinetex C(18)) and conventional liquid chromatography (LC) with a 3 μm porous particle column (Atlantis dC(18)), coupled with electrospray ionization tandem mass spectrometry (ESI-MS/MS), for the determination of 151 pesticides in grains is presented in this study. Pesticides were extracted from grain samples using a procedure known as QuEChERS (quick, easy, cheap, effective, rugged, and safe). Quantification, with an analytical range from 5 to 500 μg/kg, was achieved using matrix-matched standard calibration curves with isotopically labeled standards or a chemical analogue as internal standards. The method performance parameters that included overall recovery, intermediate precision, and measurement uncertainty were evaluated using a designed experiment, that is, the nested design. The UHPLC (Kinetex C(18)) was superior to conventional LC (Atlantis dC(18)) as it yielded a shorter analytical run time, increased method sensitivity, and improved method performance. For UHPLC/ESI-MS/MS (Kinetex C(18)), 90% of the pesticides studied had recoveries between 81 and 110%, 88% of the pesticides had intermediate precision ≤20%, and 84% of the pesticides showed measurement uncertainty ≤40%. As compared to UHPLC/ESI-MS/MS (Kinetex dC(18)), the LC/ESI-MS/MS (Atlantis dC(18)) showed a relatively lower sensitivity, less repeatability, and larger measurement uncertainty. UHPLC/ESI-MS/MS with 2.6 μm core-shell particle column and scheduled MRM proved to be a good choice for quantification or determination of pesticides in grains.     

Addressing electric utility surface water challenges

Growing regulatory pressures and increasing public concerns regarding a broad range of utility surface water management issues have the potential to substantially impact electric utility operations and costs. In order to pro-actively contribute and respond to the increasing number and scope of regulatory proposals, the electric utility industry must develop a focused and integrated plan for addressing key information and analytical needs. Over the past year, EPRI has conducted an extensive review of current and planned surface water-related research with the goal of targeting future research activity towards the highest priority industry needs. As a result, new or expanded research efforts in the areas of toxics sampling and analysis, field measurements of aqueous process and discharge streams, cost-effective metals control technologies, bioaccumulation and bioavailability of toxics, and watershed-wide risk assessment and management, are planned. The new initiatives will build on and complement current surface water research programs, as well as leverage recent efforts addressing utility stack emissions of trace substances (or air toxics). A risk management framework will be used to integrate the diverse research inputs and apply them to address key policy and utility management issues. This framework, and the tools and data developed through current and subsequent research efforts, will provide the comprehensive, multi-media data and analysis tools needed to respond to current and future utility environmental management challenges efficiently and cost effectively.     

Rainwater Composition and Acidity at Five Sites in Malaysia, in 1996

Rainwater chemical composition and acidity were determinedfrom weekly-averaged wet-only rainwater samples collected atfive sites in Malaysia throughout 1996. The major aim of thiswork was to assess the extent to which acid deposition, foundpreviously at one site in the Klang Valley, was a general,rather than local, phenomenon. To this end, three measurementsites were located spanning the length of the Klang Valley (anurban-industrial region of approximately 3000 km²containing the Malaysian capital, Kuala Lumpur). A fourth sitewas located outside, but adjacent to the Klang Valley, and thefinal site was located 300 km to the southeast, in anurban/industrial region adjacent to Singapore. Annual pHvalues in the range 4.16–4.40 and estimated total depositionfluxes of acidic sulfur and nitrogen species in the range 120–350 meq m^-2 yr^-1 show all sites to be impactedsignificantly by acidic deposition. Average contributionswere 60% as sulfur species, 40% as nitrogen species. Theresults confirm that potentially significant levels of aciddeposition occur in Malaysia as a regional phenomenonassociated with urban/industrial activities.     

Hybridization of Desmodium species

Summary Under different light regimes, Desmodium canum (Gmel.) Schin. & Thell. responded as a long-day plant, D. intortum (Mill.) Urb. and D. uncinatum (Jacq.) dc. as short-day plants, and D. sandwicense E. Mey. as photoperiod-insensitive. Tripping method with anther removal, washing of stigmas and pollen transfer was simple and less time-consuming, gave an equal number of hybrids but more selfed progenies than the tedious method of emasculating prior to anthesis. A high percentage of selfing indicated that the four species are self-compatable and that foreign pollen did not show advantage over that from the same plant. Silver marking of the leaflet midrib of D. sandwicense and D. uncinatum in crosses with D. intortum was a useful dominant marker, but did not appear in the cross D. canum × D. uncinatum. Brown flecking of D. intortum leaflet was a dominant characteristic and occurred on the progenies from crosses with D. sandwicense and D. uncinatum along with the silver marking. Stem growth habit, leaflet form, and pubescence were helpful but less reliable for identifying hybrids. F₁ hybrids were readily verified by isozyme patterns of esterase and peroxidase, the zymograms having common and different bands of the parents.Plant Breeding Paper No 639.     

Development of a more market-oriented economy in china

Before 1978 in China, the economic institutions for agriculture and industry operated essentially under a centrally planned system. The reasons for a change toward a more market-oriented economy and the key elements of economic reform are discussed. Today the major issues being deliberated by the leading economic officials include reform of the price system, the administrative structure of state-owned enterprises, the banking system and macroeconomic control mechanisms, and foreign trade and investment.     

Surgical Management of Rabbit Ear Disease

Clinical signs associated with otic disease in rabbits (Oryctolagus cuniculus) are very similar to those demonstrated in dogs and cats. The surgical treatment options to resolve diseases involving the rabbit ear are also comparable with those used for canine and feline species. However, there are key anatomic and physiologic differences that must be accounted for to perform proper surgical treatment on a rabbit that has been diagnosed with ear disease. This article describes the diagnostic and treatment options for otitis externa, otitis media, and otitis interna, with an emphasis on surgical procedures and the important distinctive otic features of the rabbit patient. The ventral bulla osteotomy procedure is recommended for management of otitis media in rabbits without otitis externa. In rabbits with pathology in the external ear canal and the middle ear, total ear canal ablation and lateral bulla osteotomy should be performed. This combined procedure will allow the surgeon to address pathology in both sites, with the ultimate goal of resolution of the underlying disease etiology. Lateral ear canal resection is only appropriate in rabbits with otitis externa without otitis media; however, this surgical procedure may not resolve the otitis externa. If this is a concern, a total ear canal ablation should be done because it offers a greater chance of success.     

First capture of post-spawning female of the Japanese eel Anguilla japonica at the southern West Mariana Ridge

Two adult male freshwater eels, Anguilla japonica, were captured in June 2008 in the West Mariana Ridge (13°N, 142°E) in the North Pacific, but collections of females have yet to be reported. In September 2008, we successfully caught two adult female A. japonica, 55.5 and 66.2 cm in total length, in the adjacent but northern area (14°N, 143°E). Six newly hatched eel larvae (pre-leptocephali) were also collected by subsequent plankton sampling conducted near the female catch area. Female adults appeared to be in the post-spawning state, probably a considerable time after spawning, since a small number of remarkably regressed oocytes (50–250 μm in diameter) were observed in the ovaries. Capture of post-spawning female eels and newly hatched larvae near the Suruga Seamount (14°N) together with the previous collection of mature males in the southern area (13°N) corroborates that the area along the West Mariana Ridge is the spawning area of this species, but suggests that the eel spawning may occur over a wider area than previously expected.     

The bioefficacy of microemulsified natural pigments in egg yolk pigmentation

1. This study was designed to test the hypothesis that microemulsified carotenoid products show improved bioavailability over corresponding regular preparations, leading to greater yolk pigmentation at lower dosages.

2. The first trial was conducted using a maize–soya bean basal diet supplemented with either 0.25, 0.5, 0.75, 1.0 and 1.25 g/kg of microemulsified Red or non-microemulsified Red. The second trial involved feeding microemulsified Yellow or non-microemulsified Yellow using a similar dosage range. The layers were divided into 4 replicates of 8 layers each (32 layers per treatment). The 8 cages of layers were fed from a single feed trough. Feed and water were provided ad libitum throughout the trial. Each week, the eggs were collected. The whole liquid egg colour was determined by means of a commercially available yolk colour fan. Where required, HPLC-(high-performance liquid chromatography) based analysis of trans-capsanthin or trans-lutein equivalents using the Association of Analytical Communities method was carried out. Data were statistically analysed by one-way ANOVA method using Statgraphics.

3. Results showed that the colour and carotenoid content of the egg yolk increased with increasing amount of carotenoids in the diet. The colour of egg yolks from layers fed similar concentrations of microemulsified versus the regular preparation was significantly different. At the commercial recommended dose of one g/kg regular Yellow or Red product, the microemulsified pigmenter is able to provide the equivalent yolk colour at a 20–30% lower dose.

4. In conclusion, the trial results supported the hypothesis that a desired yolk colour score is achievable at a significantly lower inclusion rate when carotenoid molecules are emulsified using the microemulsion nanotechnology.