东亚飞蝗蝗蝻抽样的研究

自五十年代以来,应用于东亚飞蝗蝗区估计蝗蝻种群密度的抽样方法,是样方为10平方尺的筒单随机抽样。本文是以抽样数、抽样消耗、相对精确度为标准,对三种抽样模型、三个抽样单位进行了比较。根据这些结果,我们提出了在当前蝗蝻密度较低的情况下,应采用样方为100平方尺的最适配额分层抽样。这种抽样方法在精确度方面可以高出现用的抽样方法2倍以上。而抽样时间消耗可相对节约一半以上。     

The Blood-Vessel System of the Equine Periodontal Ligament: a Mesoscopic Study on Corrosion Casts

Several investigators have suggested that the blood-vessel system in the equine periodontal ligament does not only serve nutritional needs, but also specific functional needs, e.g. in terms of a shock absorbing system. In order to supplement the previous data, corrosion casts of the periodontal blood vessels were studied with special attention to the spatial arrangement and connections of the vessels. The heads of eight warm-blooded horses (age 2.5–23 years, seven female, one male), which had been killed for medical reasons, were dissected. The arteria alveolaris inferior and the a. infraorbitalis were perfused with 100 ml Heparin (20 000 IU) followed by 100 ml tap water. After storage at 4°C for 24–96 h, 10–200 ml methacrylate resin (Biodur^TM E20) were injected by use of a cartridge pistol for approx. 1 h. After polymerization for 24 h at 60°C, the jaws were frozen and cut with a steel band saw in a horizontal, sagittal or transversal plane to obtain segments that exposed the periodontal space. The specimens were macerated in detergent at 60°C until the soft tissues had vanished. Care was taken to keep the periodontal vessels complete and in topographical integrity. The vascular casts were mesoscopically examined by use of a dissection microscope (magnification 5.8×–40×). The blood vessels in the periodontal space connected with vessels in three other sites:

• (1)

  occlusal – with the blood vessels of the gingiva;

       

大蚕、蛹组织切片核酸特异染色技术的探讨

本文对大蚕、蛹的石蜡切片制作及其核酸特异染色方法进行探讨，针对虫体大和蛹体壁高度丁质化特点，对组织固定，脱水、透明、透蜡的时间相应的增加和固定前蛹皮剪开，在截位时要考虑切片段两头保留一段仅用来起保护作用的部分，并采用了二种核酸特异染色方法染色。结果表明：制片设计方案基本可行，部分细节需进一步改进；甲基绿-派罗宁（昂纳-帕彭海姆）染色法的切片组织颜色层次分明，DNA含量丰富的丝腺组织呈紫黑色，其它组织的DNA呈紫红色，RNA呈淡红色，改良的甲基绿一派罗宁染色法的切片组织颜色偏淡；孚尔根染色法的切片组织颜色层次分明，核酸呈紫红色，其它无色，能较好的以颜色的深浅来判断核酸的丰度。更理想的染色方案还有待进一步摸索。     

组氨醇脱氢酶抑制剂的研究动态

组氨醇脱氢酶(HDH)是组氨酸生物合成过程中最后两步反应的催化酶。对组氨醇脱氢酶的结构、催化机理、组氨醇脱氢酶抑制剂等方面的研究动态进行了较为详细的综述。     

变叶海棠种群多样性的形成与分化研究

 采用杂种指数和形象化散点图法, 探明了变叶海棠以兼性无融合生殖方式与陇东海棠和花叶海棠产生渗入杂交变异, 又以无融合生殖方式保持新产生变异的遗传, 经过数千万年的世代繁衍,形成现今这样极其复杂多样的渗入杂交群体, 该群体为3 个种的杂交复合体。由于杂种的全部或部分能育子代中发生的基因重组和增加基因的突变, 丰富了物种的基因库, 从而深入的揭示了变叶海棠具有多种高抗性的遗传机理。在变叶海棠居群内保护或栽植适量的陇东海棠和花叶海棠, 对于保持和促进变叶海棠多样性的形成与分化具有重要意义。     

早熟优质长豇豆新品种‘鄂豇豆2 号’

 ‘鄂豇豆2号’是经有性杂交选育而成的早熟蔓生豇豆新品种。该品种荚浅绿色, 长60 cm,单荚质量18~ 24 g。早熟, 荚商品性状优良, 产量高, 对光周期反应不敏感, 田间疫病、煤霉病、病毒病发病率低, 适宜于春、夏、秋各季栽培, 已在全国大部分省份推广种植成功。     

杜鹃花新品种‘朝晖’和‘红晕’

 ‘朝晖’杜鹃花是由大白花杜鹃和马缨花杂交选育而成, 叶面灰绿色, 顶生圆锥伞房花序, 有花10～12 朵, 花冠紫红色。‘红晕’杜鹃花是由露珠杜鹃和马缨花杂交选育而成, 叶浅绿色, 花冠漏斗状钟形, 花冠上部粉红色, 下部乳黄色, 有紫红色斑点。     

江汉平原水稻白叶枯病菌致病型的变异研究

 从水稻和稻田周围常见的10种杂草上来集梨孢属菌株,以涂沫法作交互接种,分离自牛筋草、狗尾草、法氏狗尾草、李氏禾、罗氏草、铺地黍、稗和马唐的梨孢菌与分离自水稻的梨孢菌可以交叉侵染其寄主,茭白和碎米莎草梨孢菌与稻梨孢菌不能交互侵染;自然诱发条件下来自牛筋草和狗尾草的梨孢菌与来自水稻的梨孢菌对其寄主同样发生交叉侵染。直接从杂草寄主上分离的梨孢菌株对水稻的致病性较弱,接种到水稻感病品种发病后再分离的菌株,对水稻致病性增强,对原寄主的致病性基本保持不变。推断稻瘟病区稻田周围牛筋草、狗尾草、法氏狗尾草、李氏禾、罗氏草、铺地黍、稗和马唐等杂草寄主上的梨孢菌在稻瘟病菌的积累和传播方面具有作用。