一例鸭鼠伤寒沙门氏菌的分离鉴定及抗原性分析

对出现精神沉郁、食欲减退、拉稀、脚软和共济失调症状,其后死亡,的鸭病例,进行常规诊断、动物试验,结果分离到一株细菌,命名为GDYJS-1.分离株通过生长特性、凝集试验、染色特性及VITEK-32微生物鉴定系统生化试验鉴定为沙门氏菌;16S rRNA基因序列的测定与分析,鉴定为鼠伤寒沙门氏菌.     

赤羽病SYBR Green I实时荧光PCR方法的建立和应用

本研究根据赤羽病毒（AKAV）的S基因序列设计合成了引物, RT-PCR扩增得到目的基因片段并克隆到PGM-T载体得到质粒标准品。通过对荧光定量PCR反应条件的优化,建立了SYBR Green I荧光定量PCR检测AKAV的方法。该方法的检测敏感性达到926拷贝/25μL,并具有较好的重复性和特异性。本试验方法的建立对于加强进出口牛AKAV的检验检疫具有十分重要的意义。     

Optimizing the Conditions for In Vitro Maturation and Artificial Activation of Sika Deer (Cervus nippon hortulorum) Oocytes

With the goal of establishing experimental protocols for cloning sika deer, various conditions for in vitro maturation (IVM) and artificial activation of sika deer oocytes were examined. In vitro maturation was evaluated in seven different culture media. The highest rate of oocyte maturation was 75.4% in 10 μg/ml follicle‐stimulating hormone (FSH), 1 μg/ml LH, 0.2 mm cysteamine and 50 ng/ml epidermal growth factor (EGF) after 24 h of IVM. The efficiency after 24 h of IVM did not differ significantly (p > 0.05) from that observed after 20 h. Cysteamine (0.2 mm ) significantly increased the maturation rates after 20 h (from 59.1% to 67.2%, p < 0.05) and after 24 h (from 63.2% to 71.6%, p < 0.05) of IVM. The IVM rates of oocytes collected during the oestrous season (75.4%) and the anoestrous season (23.3%) were significantly different at 24 h. The 20 μg/ml FSH, 2 μg/ml LH, 0.4 mm cysteamine and 100 ng/ml EGF significantly increased the maturation rates (from 23.3% to 54.2%, p < 0.01) at 24 h during the anoestrous season. For the activation experiments, the most effective method was chemical activation [ionomycin + 6‐dimethylaminopurine (6‐DMAP)], which promoted the development of sika deer oocytes to the blastocyst stage (32.4%). Our results indicate that in vitro matured sika deer oocytes are good candidates for parthenogenetic activation and that chemical treatment is needed for relatively efficient activation of the oocytes. These optimized conditions for IVM and parthenogenetic activation may be useful for efforts to restore populations of the endangered sika deer using the somatic cell nuclear transfer technique.     

乡镇综合变以下智能表与集中器故障分析及对策

重点对江苏省乡镇供电所辖区内综合变以下智能电能表与低压用电采集系统集中器进行简单介绍,阐述了其基本工作原理以及使用过程中遇到的一些常见故障及相关分析,同时列举乡镇供电所在日常工作排查消缺中遇到的一些实例并进行重点分析。     

新时期农业经济管理的分析与研究

农业经济是我国重要的经济支柱,加强对农业经济的管理,对保证我国的国民经济利 益具有重要的作用。基于此,本文就主要研究新时期的农业经济管理,对开展农业经济管理的 意义进行讨论,并找出管理中存在的问题,然后提出一系列的优化措施和问题解决措施。     

水盐梯度对滨海湿地土壤养分指标和酶活性的影响

[目的]研究海水入侵对滨海湿地土壤性质的影响,为分析水盐梯度对土壤养分指标和酶活性的影响机制提供依据。[方法]采集胶州湾滨海湿地表层土壤并设计室内模拟试验,测定不同水盐梯度上的土壤养分指标和酶活性。[结果]盐分和水分对土壤pH值和容重(BD)的影响显著;土壤有机质(TOM)、氨氮(NH_4~+-N)、速效磷(AP)、速效钾(AK)、蔗糖酶(SA)、脲酶(UA)、碱性磷酸酶(APA)均随水梯度的增加表现为先升高后降低的趋势,在30%水梯度时达到最高值;土壤TOM,AP,AK,SA,UA,APA均随盐梯度的增加而降低,NH_4~+-N随盐梯度的增加而增加。土壤SA与TOM,AP,AK存在显著正相关关系;APA与TOM,AP,AK,BD显著正相关;UA与pH值,BD显著正相关,与NH_4~+-N具有一定的相关性。[结论]在一定范围内,土壤中水分过高或过低、盐分增加均会对土壤养分含量和酶活性产生抑制作用;土壤养分和酶活性之间存在密切的相关关系。     

Evaluation of the Effect of Sprout Soybeans on the Iron Status of Anemic Adolescent Girls in Rural China

Plant Foods for Human Nutrition - Soybeans are a major source of nonheme iron in Chinese diet. Germination is considered to be effective in improving iron bioavailability in soybeans. The study is...     

多维产业引导的鄂西生态圈农村居民点整理模式及分区

农村居民点承载了农户生产和生活的重要功能,是农村人地关系的核心表现。针对中国农居点分布散乱、经济贫困和功能性弱的现状,开展因地制宜的整理模式研究将有助于推动乡村空间重构进程。产业发展作为乡村振兴的核心动力,是农村居民点整理规划中的关键要素。现有关于产业与整理模式的研究多强调生产空间的可达性、适宜性与协调性,较少探讨产业现状及其发展前景所产生的多维引导与带动作用。该研究面向中国鄂西生态圈农村居民点落后散乱以及生态农业潜力大、水平低的特点,选取鹤峰县邬阳乡为研究区,基于产业引导、统筹协调的思路,从产业发展、生活便利和生态健康3个方面综合评价农居点发展潜力,并在此基础上,从产业现状水平、产业辐射能力与产业融合潜力3个维度出发,开展顾及产业现状影响与多元发展前景的农居点整理模式识别分析。研究结果表明,试验区整理模式主要包括统筹协调发展型、产业稳定发展型、产业辐射带动型、产业融合引导型和产业调整培育型5种,在空间上以邬阳村为中心向外呈现圈层分布,存在多样化的未来发展格局。差异化的农村居民点整理模式是确保其实际落地的关键,该研究通过结合地域特征及区域规划,构建的多维产业引导下的农居点整理模式及分区,对创新农村居民点整理模式理论与实践有一定的指导意义,有望助力"乡村振兴"战略和"产业扶贫"政策的具体实施。     

Association of antioxidant vitamins and oxidative stress levels in pregnancy with infant growth during the first year of life

OBJECTIVE: Whereas there are numerous reports in the literature relating the impact of maternal nutritional status on subsequent birth outcome, much less is known about the long-term impact on infant growth after birth. Therefore, we conducted a prospective cohort study to investigate the association of maternal micronutrient status (vitamins A, C and E, folate) and oxidative stress status in pregnancy with infant growth during the first year of life. DESIGN: Prospective cohort study. SETTING: Outpatient clinic of obstetrics, Ewha Womans University Hospital, Seoul, South Korea.Subjects and methodsTwo groups were constructed for this study - the Ewha pregnancy cohort (n = 677) and the infant growth cohort comprising follow-up live newborns of all the recruited pregnant women (n = 317). Maternal serum vitamin and urinary oxidative stress levels were collected and infant weights and heights were measured at birth and at 6 and 12 months after birth. RESULTS: Division of the subjects into folate-deficient and normal groups revealed that infant weight and height at 0, 6 and 12 months were adversely affected by folate deficiency. High maternal vitamin C was associated with increased infant weight and height at birth and after birth.ConclusionOur findings indicate the importance of preventing folate deficiency and supplementing vitamin C during pregnancy.