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101.
102.
Galina P. Simeonova Svetozar Z. Krastev Radostin S. Simeonov 《Veterinary research communications》2016,40(3-4):107-115
Background
The pathogenic mechanism of equine recurrent uveitis (ERU) is still poorly defined and many variations between experimental animal models and spontaneous disease exist.Objectives
The aim of our study was to investigate if Th17 cell-mediated response plays role in the pathogenesis of the used experimental model in horses and to reveal its pathological findings.Methods
Experimental uveitis was induced in 6 healthy horses. The concentrations of retinal autoantigen CRALBP and IL-17 were measured using ELISA in aqueous humor and vitreous body of the 12 inflamed eyes as well as in 12 control non-inflamed eyes taken from 6 horses in slaughter house. After centrifugation of the two eye media, smears were prepared and cytological investigation was performed. Tissue specimens were taken from all eye globes and were submitted to histopathological investigation.Results
CRALBP and IL-17 concentrations were significantly elevated in eye media of horses with experimental uveitis in comparison with controls. Cytological and histopathological findings corresponded to the changes characteristic of chronic immune-mediated inflammation with mononuclear cell infiltration of uvea, choroid, retina, and eye media as well as severe retinal destruction.Conclusions
Our study demonstrated the involvement of the retinal autoantigen CRALBP as well as IL-17 in the pathogenesis of experimental uveitis in horses. These findings suggests that this experimental uveitis in horses may serve as a suitable animal model for investigation of IL-17- mediated immune response during spontaneous autoimmune uveitis in horses as well as in humans.103.
A peptide of foot-and-mouth disease virus serotype Asia1 generating a neutralizing antibody response, and an immunostimulatory peptide 总被引:2,自引:0,他引:2
Wang JL Liu MQ Han J Chen WZ Cong W Cheng G Gao YH Lu YG Chen JL Zuo XP Yan WY Zheng ZX 《Veterinary microbiology》2007,125(3-4):224-231
The epitopes of the capsid of foot-and-mouth disease virus (FMDV) play important roles in the construction of highly immunogenic subunit vaccines. However few epitopes have been found for FMDV serotype Asia1. In this study we screened for epitopes of the VP1 and VP2 proteins of FMDV serotype Asia1 isolate, YNBS/58. Fragments consisting of amino acids 133-163 of VP1 and amino acids 1-33 of VP2 contained epitopes, and both induced lymphoproliferation in guinea pigs. Only the VP1 fragment induced neutralizing antibodies but the VP2 peptide dramatically increased the neutralizing antibody response induced by the VP1 peptide. 相似文献
104.
Piao SJ Tsukahara T Itoh M Shiga A Adachi Y Ushida K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(4):425-428
We observed a significant difference in the organic acid profile of diarrheal feces between pigs infected with and free from pathogenic spirochetes. Diarrhea and loose feces were collected from growing pigs, held at 15 different commercial farms. A total of 106 samples were measured for organic acid concentration by HPLC and were checked for the presence of B. hyodysenteriae and B. pilosicoli by PCR. B. hyodysenteriae was detected in 3 samples collected from one farm. B. pilosicoli was detected in 5 samples collected from another farm. Lower concentrations of iso-butyrate and iso-valerate were likely associated with development of pathogenic spirochete infection. 相似文献
105.
本研究通过细胞融合、间接ELISA方法筛选出4株可稳定分泌犬瘟热病毒(canine distemper virus,CDV)和犬细小病毒(canine parvovirus,CPV)单克隆抗体的杂交瘤细胞株,以此为基础研制了一种可同时检测CDV和CPV的胶体金二联检测卡,并对其特异性、灵敏度及准确性进行了测试。结果显示:4株杂交瘤细胞可稳定传代,分泌的单克隆抗体纯度高,效价均在1:320以上。制备的胶体金二联检测卡特异性强,只与CDV和CPV产生特异性条带,而不与其他犬类病毒反应;灵敏度高,最低检测限可达到102拷贝/μL;准确性好,与荧光定量PCR结果的符合率高于88.0%,与市面上单一病毒检测卡的符合率高于97.2%。结果表明,本研究制备的CDV、CPV胶体金二联检测卡特异性强、灵敏度高、准确性好,兼具操作简便、肉眼可判读、结果易保存和无需特殊仪器设备等优势,可用于两种疾病的临床快速诊断和大规模检测工作。 相似文献
106.
107.
本文介绍一种用于分离检测土壤中棉枯萎病菌的选择性培养基—植选2号。其成分为:KH_2PO_41g,MgSO_4·7H_2O 0.5g,K_2S_2O_50.2g,KCl0.6g,NH_4NO_3 0.5g,蛋白胨5g,山梨糖10g,蔗糖5g,琼脂20g,蒸馏水1000ml,PCNB 620mg,Oxgall1g,硫酸链霉素300mg,盐酸金霉素75mg。根据棉枯萎病在此培养基上的形态特征,能较容易地识别和检测该病菌。 相似文献
108.
109.
This article presents an implement of component using COM technology for collecting environmental monitoring data in environment monitoring system. The component makes the separation of data collection and data analysis available.Simultaneously by using the interface of connectable object in COM,itmakes the cooperation of the environment monitoring software with other parts of fault management software available,and also the seamless connection.It improves the system's performance by adding extensibility and potential cooperatibility. 相似文献
110.
In order to research the state of energy bands and gain and loss of electrons on the surface of ZnSnO_(3),it is put forward that Pinning theory of Fermi energy is applied to development of gas sensitive materials.The relative position between surface state and conduction band on surface of zinc tin complex oxide(ZnSnO_(3)) gas sensitive material has been investigated by utilizing oxidation redox couple,FeCl_(2)/ FeCl_(3),which have high surface state.Fermi energy is pinned at about 0.2 eV below conduction band E_(cs)on the surface of ZnSnO_(3).It has been determined that additives doping ratio,addition concentration and working atmosphere have no effect on this value.Therefore,pinning theory of Fermi energy has been verified by using this method. 相似文献