利用转录组测序技术分析ADC和NOS基因mRNA在鹅等级前卵泡中的表达

为研究精氨酸代谢的关键酶精氨酸脱羧酶(Arginine Decarboxylase,ADC)和一氧化氮合酶(Nitric-oxide Synthase,NOS)mRNA在鹅等级前卵泡中的表达情况。采用高通量测序技术对鹅等级前卵泡进行转录组测序分析,并通过荧光定量PCR对结果进行验证。通过FPKM值计算分析,这2个基因在鹅等级前卵泡中均有表达,且随着卵泡的发育,NOS mRNA的表达呈现先降低后增加再降低的趋势,在初级卵泡中的表达量达到最高值,在中白卵泡中的表达量最低。ADC mRNA的表达则呈现先增加后降低再增加的趋势,在初级卵泡中的表达量最低,在小白卵泡中的表达量最高。荧光定量PCR结果与转录组测序结果基本相同。     

Expression in Escherichia coli of open reading frame gene segments of HTLV-III

Human T-cell lymphotropic virus type III (HTLV-III), the causative agent of the acquired immune deficiency syndrome (AIDS), was recently isolated and its genomic structure analyzed by DNA cloning methods. In the studies reported here a combined cloning and expression system was used to identify HTLV-III encoded peptides that react immunologically with antibodies in sera from AIDS patients. Cloned HTLV-III DNA was sheared into approximately 500-base-pair fragments and inserted into an "open reading frame" expression vector, pMR100. The inserted DNA was expressed in Escherichia coli transformants as a polypeptide fused to the lambda CI protein at its amino terminus and to beta-galactosidase at its carboxyl terminus. Sera from AIDS patients containing antibodies to HTLV-III were then used to screen for immunoreactive fusion proteins. Twenty clones, each specifying a fusion protein strongly reactive with AIDS serum, were identified. DNA sequence analysis indicated that the HTLV-III fragments were derived from the open reading frame DNA segments corresponding to the gag and pol gene coding regions and also the large open reading frame region (env-lor) located near the 3' end of the viral genome.     

中草药添加剂饲喂猪肉中CPK,GPI活性和肉质关系的研究

 对添加中草药和常规抗生素试验猪的背最长肌进行相关酶测定,发现磷酸肌酸激酶（CPK）,磷酸葡萄糖异构酶（GPI）的活性,试验组明显高于对照组,分别提高了30.54%和20.04%（P＜0.05）.屠宰及肉质试验表明,试验组的瘦肉率及眼肌面积分别比对照组提高5.79%和8.48%（P＜0.05）,而脂肪率、膘厚、失水率和贮存损失则分别下降31.40%,28.21%,50.03%和20.35%（P＜0.05）.     

基于XPS的苹果表皮残留农药界面层的氩刻分析

针对果皮表面残留农药进行微观深度探测及分析。结果表明,苹果表皮氧乐果及敌敌畏随施药浓度及施药时间的残留沿纵向深度变化呈现非线性规律性,主要残留部位为果实表皮的角质层区距表面8μm,氧乐果残留分布的规律性强于敌敌畏。     

汶川特大地震灾后山洪灾害预估与应对措施

“5·12”汶川特大地震造成的灾区地表松动,使处于雨季的灾区面临山洪灾害的严峻挑战。通过对比历史上灾区和近邻地区已发生山洪灾害的历史状况,分析灾区山洪灾害的重点防范月份,绘制灾区山洪灾害高、次高、中、低易发区的分布位置,提出了不同风险区防范山洪灾害的应对措施,供汶川特大地震灾后山洪灾害预警参考。     

施氮处理对不同筋型小麦产量和品质的影响

为探究施氮处理对不同筋型小麦的植株性状、籽粒产量和品质的影响,采用盆栽试验,选用强筋小麦品种中麦578（A1）和中麦5051（A2）、弱筋小麦品种扬麦15（A3）和扬麦24（A4）为供试品种,在施氮量相同的条件下进行底施（B1）和追施（B2）处理。结果表明：在其他栽培措施相同条件下,强筋小麦穗长、总小穗数、千粒重和籽粒产量均优于弱筋小麦,其中A1籽粒产量分别比A2、A3和A4高0.44%、49.81%和15.27%;施氮处理中B2的株高、穗长、穗粒数、总小穗数和籽粒产量均高于B1;不同处理组合中,强筋小麦品种A1B2的植株和产量性状优于其他处理;强筋小麦品种的籽粒蛋白质含量和蛋白质产量均高于弱筋小麦品种,且具有极显著差异（P<0.01)。本试验中强筋小麦品种中麦5051在氮肥追施处理中可以兼顾籽粒产量、蛋白质含量和蛋白质产量。     

深圳市游憩资源现状及建设对策研究

文中采用问卷调查的方式结合对游憩资源的空间分布研究,对深圳市目前游憩资源的供给能力、使用状况、服务能力以及公众满意度与影响原因进行分析,结果表明:1)深圳总体的游憩资源类型供给能力排序为滨海风光类 > 都市观光类 > 休闲娱乐类 > 人文历史类 > 自然郊野类,同时各区的供给能力及其对总体资源供给能力的贡献不相同,盐田海滨风光类供给能力强且贡献最大,南山、福田、罗湖都市观光类供给能力强且对贡献相对次之,龙岗自然资源供给能力最强但贡献较小;2)海滨风光、都市观光、自然郊野游憩资源具有较高的吸引力与使用度;3)深圳总体自然郊野游憩资源的服务压力最大,其次为都市观光、滨海风光、休闲娱乐、人文历史,同时不同区的游憩资源本地服务能力存在差异;4)公众对深圳总体游憩地的满足度还不够高,主要源自建设内容与形式、特色创新、亲和融入性、体验参与性、方便可达性5个方面存在的问题。最后基于这一结果归纳出各区游憩资源开发提升的主题并提出多样丰富性、特色创新性、亲和融入性、体验参与性、方便可达性5项建设原则。     

螺旋式胡椒鲜果脱皮机的设计与试验分析

为解决现有胡椒鲜果脱皮机脱皮不彻底导致含杂率高、脱皮力不当导致果核破碎及果核保护膜破损导致白胡椒色泽不理想等问题,在分析现有胡椒机械加工工艺的基础上,研制出螺旋式胡椒鲜果脱皮机。该机通过螺旋网面挤压胡椒鲜果,胡椒鲜果表皮在螺旋网面及容器内壁发生摩擦作用以完成脱皮。试验结果表明:螺旋式胡椒鲜果脱皮机的脱皮率达93%以上,且果核损伤率低于3%,符合设计要求。     

Melatonin reduces apoptotic cells,SOD2 and HSPB1 and improves the in vitro production and quality of bovine blastocysts

Effects of adding different concentrations of melatonin (10^?7, 10^?9 and 10^?11 M) to maturation (Experiment 1; Control, IVM  + 10^?7, IVM  + 10^?9, IVM  + 10^?11) and culture media (Experiment 2; Control, IVC  + 10^?7, IVC  + 10^?9, IVC  + 10^?11) were evaluated on in vitro bovine embryonic development. The optimal concentration of melatonin (10^?9 M) from Experiments 1–2 was tested in both maturation and/or culture media of Experiment 3 (Control, IVM  + 10^?9, IVC  + 10^?9, IVM /IVC  + 10^?9). In Experiment 1, maturated oocytes from Control and IVM  + 10^?9 treatments showed increased glutathione content, mitochondrial membrane potential and percentage of Grade I blastocysts (40.6% and 43%, respectively). In Experiment 2, an increase in the percentage of Grade I blastocysts was detected in IVC  + 10^?7 (43.5%; 56.7%) and IVC  + 10^?9 (47.4%; 57.4%). Moreover, a lower number and percentage of apoptotic cells in blastocysts were observed in the IVC  + 10^?9 group compared to Control (3.8 ± 0.6; 3.6% versus 6.1 ± 0.6; 5.3%). In Experiment 3, the IVC  + 10^?9 treatment increased percentage of Grade I blastocysts with a lower number of apoptotic cells compared to IVM /IVC  + 10^?9 group (52.6%; 3.0 ± 0.5 versus 46.0%; 5.4 ± 1.0). The IVC  + 10^?9 treatment also had a higher mRNA expression of antioxidant gene (SOD 2) compared to the Control, as well as the heat shock protein (HSPB 1) compared to the IVM  + 10^?9. Reactive oxygen species production was greater in the IVM /IVC  + 10^?9 treatment group. In conclusion, the 10^?9 M concentration of melatonin and the in vitro production phase in which it is used directly affected embryonic development and quality.