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71.
Vania Cristina Desoti Danielle Lazarin-Bidóia Daniela Bueno Sudatti Renato Crespo Pereira Tania Ueda-Nakamura Celso Vataru Nakamura Sueli de Oliveira Silva 《Marine drugs》2014,12(9):4973-4983
Chagas’ disease, a vector-transmitted infectious disease, is caused by the protozoa parasite Trypanosoma cruzi. Drugs that are currently available for the treatment of this disease are unsatisfactory, making the search for new chemotherapeutic agents a priority. We recently described the trypanocidal action of (−)-elatol, extracted from the macroalga Laurencia dendroidea. However, nothing has been described about the mechanism of action of this compound on amastigotes that are involved in the chronic phase of Chagas’ disease. The goal of the present study was to evaluate the effect of (−)-elatol on the formation of superoxide anions (O2•−), DNA fragmentation, and autophagy in amastigotes of T. cruzi to elucidate the possible mechanism of the trypanocidal action of (−)-elatol. Treatment of the amastigotes with (−)-elatol increased the formation of O2•− at all concentrations of (−)-elatol assayed compared with untreated parasites. Increased fluorescence was observed in parasites treated with (−)-elatol, indicating DNA fragmentation and the formation of autophagic compartments. The results suggest that the trypanocidal action of (−)-elatol might involve the induction of the autophagic and apoptotic death pathways triggered by an imbalance of the parasite’s redox metabolism. 相似文献
72.
Dos Santos AO Britta EA Bianco EM Ueda-Nakamura T Filho BP Pereira RC Nakamura CV 《Marine drugs》2011,9(11):2369-2383
Natural marine products have shown an interesting array of diverse and novel chemical structures with potent biological activities. Our study reports the antiproliferative assays of crude extracts, fraction and pure compound (4R,9S,14S)-4α-acetoxy-9β,14α-dihydroxydolast-1(15),7-diene (1) obtained from brown alga Canistrocarpus cervicornis showing the antileishmanial activity. We showed that 1 had a dose-dependent activity during 72 h of treatment, exhibiting IC(50) of 2.0 μg/mL, 12.0 μg/mL, and 4.0 μg/mL for promastigote, axenic amastigote and intracellular amastigote forms of Leishmania amazonensis, respectively. A cytotoxicity assay showed that the action of the isolated compound 1 was 93.0 times less toxic to the macrophage than to the protozoan. Additionally, compound 1 induced ultrastructural changes, including extensive mitochondrial damage; decrease in Rh123 fluorescence, suggesting interference with the mitochondrial membrane potential; and lipid peroxidation in parasite cells. The use of 1 from C. cervicornis against L. amazonensis parasites might be of great interest as a future alternative to the development of new antileishmanial drugs. 相似文献
73.
74.
José Luis Pires Júlio Cézar Mattos Cascardo Smilja Vidmar Lambert Antonio Figueira 《Euphytica》1998,103(1):115-121
Cocoa butter is the major commercial product from seeds of Theobroma cacao (cacao). However, most cacao breeding programs
have only considered as selection criteria total seed yield per plant and disease resistance. Progress in cocoa butter yield
might be possible if variability for fat content exists and its associations with the other production components are established.
The average fat content in dry unfermented seeds for 490 accessions of the Centro de Pesquisa do Cacau germplasm collection
in Ilhéus, Bahia, Brazil was 53.2%, ranging from 45.4% in CC 57 to 60.3% in NA 312. Fat content averaged over series indicated
that genotypes originally collected in the Upper Amazonian region had higher fat levels, while the Trinitario-Criollo and
Bahian genotypes tended to have lower fat content. When considering total fat yield plant-1 an opposite trend arose with the series with low fat content having the highest total fat yield. The correlation between
fat content and fat yield plant-1 was not significant, while there was a significant negative correlation between dry seed yield plant-1 and fat content. Despite the significant negative association between fat content and dry seed yield plant-1, the correlation coefficient was rather low, and genotypes could be identified with both high fat content and dry seed yield.
A significant pollen effect on fat content was observed based on a complete diallel crossing scheme between three genotypes
with high and three with low levels. The average fat content values in progenies were similar to the average of the parents,
and the segregation range transgressed the distribution of the parents.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
75.
Dietrich Pizzigatti Carlos Alberto Hussni Celso Antonio Rodrigues Marcos Jun Watanabe Juliana de Moura Alonso Luiz Carlos Vulcano Luis Emiliano Cisneros Álvarez 《Journal of Equine Veterinary Science》2013
Subluxation of the proximal interphalangeal joint is a rare and little studied condition in horses. We describe the case of a 12-year-old mare with bilateral dorsal subluxation of the proximal interphalangeal joint of the hind feet. Tenectomy of the medial digital flexor was performed in both limbs, and the patient showed signs of recovery within 14 days. Goniometry of the proximal interphalangeal joints 10 months after surgery showed diminution of 5° for the proximal interphalangeal axis of the left hind limb. However, no change was found for the joint angle of the right limb. Even so, the patient's gait and radiographic results were normal, and clinical abnormalities such as joint clicking and swelling were no longer observed. 相似文献
76.
MUC1 expression in canine malignant mammary tumours and relationship to clinicopathological features
de Oliveira JT Pinho SS de Matos AJ Hespanhol V Reis CA Gärtner F 《Veterinary journal (London, England : 1997)》2009,182(3):491-493
Mucin-1 (MUC1) is over-expressed in human breast carcinomas and is linked to a poorer prognosis. In this study, MUC1 expression in 32 spontaneous canine malignant mammary tumours was characterised in relation to histological type, mode of growth, grade, lymph node metastases and distant metastases. All tumours exhibited immunostaining for MUC1. In the normal canine mammary gland, MUC1 was expressed mainly in the apical cellular membrane, while in carcinomas MUC1 was detected in the cytoplasm only (56.3%) or in the cytoplasm with membrane accentuation (43.7%). There was a significant association between development of distant metastases and MUC1 over-expression (P=0.03), but no association with histological type, histological grade, mode of growth or lymph node metastasis. 相似文献
77.
Marisa Terezinha Garcia de Oliveira Silvia Beatriz Alves Rolim Paulo Celso de Mello-Farias Álvaro Meneguzzi Camila Lutckmeier 《Water, air, and soil pollution》2008,192(1-4):183-198
Two environmental relief compartments from Sinos Valley, Rio Grande do Sul State, Brazil, interpreted in the Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) images, are contaminated, in different ways, by two industrial landfills. One of the landfills is located in a fluvial plain with thin superficial sediments made of expansive clays. In this landfill, metals like Cr, Co, Ni, Cu, Pb, Zn, and Ba, have concentrated more than that of other landfill, which is located on a low hill in a sedimentary arenitic terrain. The metals have also accumulated in plant tissues in the investigated areas. Plants have accumulated more Mn, Zn, Ba, and Cu. 相似文献
78.
79.
Radigonda Vinicius Luiz Pereira Gabriel Ribas da Cruz Favaro Patrícia Barca Júnior Flávio Antônio Borges Marcelo Henrique Favaro Galdioli Victor Hugo Gonçalves Júnior Celso Koetz 《Tropical animal health and production》2017,49(8):1787-1791
Tropical Animal Health and Production - The objective of this study was to compare the presence of ovarian activity and pregnancy rates to temperature variation at the vulvar skin measured by... 相似文献
80.
Araújo MA Albuquerque VB Deschk M Santos GG Rodrigues CA Oliva VN Santos PS 《Veterinary anaesthesia and analgesia》2012,39(4):409-413
ObjectiveTo assess cardiopulmonary and analgesic effects after administration of ropivacaine into the caudal epidural space of cattle.Study designProspective, single-dose trial.AnimalsEight healthy mixed breed cows aged 8 ± 5 years and weighing 507 ± 112 kg.MethodsCaudal epidural anesthesia was produced in cows with 0.75% ropivacaine (0.11 mg kg?1). Onset time, duration and cranial spread of analgesia were recorded. Heart rate (HR), respiratory rate (fR), rectal temperature (RT), and mean arterial blood pressure (MAP) were measured prior to epidural administration (T0) and at 15, 30, 60, 120, 180 and 240 minutes after epidural administration (T15, T30, T60, T120, T180 and T240). Arterial blood acid-base balance (pH, standard bicarbonate and base excess), gas tension (PaO2, PaCO2, SaO2) and electrolytes (Na+, K+, iCa2+,Cl?) were recorded at T0, T30, T60, T120, T180 and T240. Ataxia was evaluated at T0, T30, T60, T120, T180 and T240 and at 1 hour intervals thereafter until analgesia was no longer present in each animal.ResultsEpidurally administered ropivacaine induced variable analgesia extending bilaterally from the coccyx to S3. Time to onset of analgesia and mean duration in the perineal area were 15 ± 4 and 359 ± 90 minutes, respectively. Respiratory rate and RT increased from T120 to T240 when compared to the value at T0. Ionized calcium and chloride concentrations increased at T180 and T240 when compared to T0. The other variables were not significantly different from baseline values (p> 0.05). Four animals were mildly ataxic.Conclusion and clinical relevanceRopivacaine (0.75%, 0.11 mg kg?1) can be administered by caudal epidural injection to produce prolonged bilateral perineal analgesia with minimal ataxia and cardiopulmonary changes in standing cattle. 相似文献