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51.
52.
Tim J Bull Christina Vrettou Richard Linedale Catherine McGuinnes Sam Strain Jim McNair Sarah C Gilbert Jayne C Hope 《Veterinary research》2014,45(1)
Vaccination is the most cost effective control measure for Johne’s disease caused by Mycobacterium avium subspecies paratuberculosis (MAP) but currently available whole cell killed formulations have limited efficacy and are incompatible with the diagnosis of bovine tuberculosis by tuberculin skin test. We have evaluated the utility of a viral delivery regimen of non-replicative human Adenovirus 5 and Modified Vaccinia virus Ankara recombinant for early entry MAP specific antigens (HAV) to show protection against challenge in a calf model and extensively screened for differential immunological markers associated with protection. We have shown that HAV vaccination was well tolerated, could be detected using a differentiation of infected and vaccinated animals (DIVA) test, showed no cross-reactivity with tuberculin and provided a degree of protection against challenge evidenced by a lack of faecal shedding in vaccinated animals that persisted throughout the 7 month infection period. Calves given HAV vaccination had significant priming and boosting of MAP derived antigen (PPD-J) specific CD4+, CD8+ IFN-γ producing T-cell populations and, upon challenge, developed early specific Th17 related immune responses, enhanced IFN-γ responses and retained a high MAP killing capacity in blood. During later phases post MAP challenge, PPD-J antigen specific IFN-γ and Th17 responses in HAV vaccinated animals corresponded with improvements in peripheral bacteraemia. By contrast a lack of IFN-γ, induction of FoxP3+ T cells and increased IL-1β and IL-10 secretion were indicative of progressive infection in Sham vaccinated animals. We conclude that HAV vaccination shows excellent promise as a new tool for improving control of MAP infection in cattle.
Electronic supplementary material
The online version of this article (doi:10.1186/s13567-014-0112-9) contains supplementary material, which is available to authorized users. 相似文献53.
Joany van Balen Jade Mowery Micha Piraino-Sandoval Rocio C Nava-Hoet Catherine Kohn Armando E Hoet 《Veterinary research》2014,45(1):31
The role that environmental contamination might play as a reservoir and a possible source of Methicillin-resistant Staphylococcus aureus (MRSA) for patients and personnel at equine veterinary hospitals remains undefined, as the environment has only been monitored during outbreaks or for short periods. Therefore, the objectives of this study were to determine the monthly presence, distribution, and characteristics of environmental MRSA at an equine hospital, and to establish patterns of contamination over time using molecular epidemiological analyses. For this purpose, a yearlong active MRSA surveillance was performed targeting the environment and incoming patients. Antimicrobial susceptibility testing, SCCmec typing, PFGE typing, and dendrographic analysis were used to characterize and analyze these isolates. Overall, 8.6% of the surfaces and 5.8% of the horses sampled were positive for MRSA. The most common contaminated surfaces were: computers, feed-water buckets, and surgery tables-mats. Ninety percent of the isolates carried SCCmec type IV, and 62.0% were classified as USA500. Molecular analysis showed that new pulsotypes were constantly introduced into the hospital throughout the year. However, maintenance of strains in the environment was also observed when unique clones were detected for 2 consecutive months on the same surfaces. Additionally, pulsotypes were circulating throughout several areas and different contact surfaces of the hospital. Based on these results, it is evident that MRSA is constantly introduced and frequently found in the equine hospital environment, and that some contact surfaces could act as “hot-spots”. These contaminated surfaces should be actively targeted for strict cleaning and disinfection as well as regular monitoring. 相似文献
54.
Rodrigo Gutierrez‐Quintana Julien Guevar Catherine Stalin Kiterie Faller Carmen Yeamans Jacques Penderis 《Veterinary radiology & ultrasound》2014,55(6):585-591
Congenital vertebral malformations are common in brachycephalic “screw‐tailed” dog breeds such as French bulldogs, English bulldogs, Boston terriers, and pugs. The aim of this retrospective study was to determine whether a radiographic classification scheme developed for use in humans would be feasible for use in these dog breeds. Inclusion criteria were hospital admission between September 2009 and April 2013, neurologic examination findings available, diagnostic quality lateral and ventro‐dorsal digital radiographs of the thoracic vertebral column, and at least one congenital vertebral malformation. Radiographs were retrieved and interpreted by two observers who were unaware of neurologic status. Vertebral malformations were classified based on a classification scheme modified from a previous human study and a consensus of both observers. Twenty‐eight dogs met inclusion criteria (12 with neurologic deficits, 16 with no neurologic deficits). Congenital vertebral malformations affected 85/362 (23.5%) of thoracic vertebrae. Vertebral body formation defects were the most common (butterfly vertebrae 6.6%, ventral wedge‐shaped vertebrae 5.5%, dorsal hemivertebrae 0.8%, and dorso‐lateral hemivertebrae 0.5%). No lateral hemivertebrae or lateral wedge‐shaped vertebrae were identified. The T7 vertebra was the most commonly affected (11/28 dogs), followed by T8 (8/28 dogs) and T12 (8/28 dogs). The number and type of vertebral malformations differed between groups (P = 0.01). Based on MRI, dorsal, and dorso‐lateral hemivertebrae were the cause of spinal cord compression in 5/12 (41.6%) of dogs with neurologic deficits. Findings indicated that a modified human radiographic classification system of vertebral malformations is feasible for use in future studies of brachycephalic “screw‐tailed” dogs. 相似文献
55.
Littoral microcrustacean (Ctenopoda, Anomopoda, Onchyopoda) samples, aquatic macrophyte data and chemical measurements were collected from 51 freshwater lochs in the Isle of Skye, Scotland, during the summer of 1989. Canonical correspondence analysis was employed to investigate correlations between the environmental data, the microcrustacean assemblages, and the aquatic macrophyte community types used for floristic classification of standing waters. Cluster analysis was used to classify lochs based on their microcrustacean assemblages. The major trends in microcrustacean distributions were related to pH, catchment area, macrophyte diversity and the distance from the sea. There was little correlation between the plant community types and microcrustacean assemblages. These results support the case for the development of an integrated approach to lake surveys so that correlations can be made between environmental variables and the distribution and abundance patterns of the plant and animal taxa of conservation interest. 相似文献
56.
Catherine Elizabeth Gibson David Williams Rebecca Dunlop Suzanne Beck 《水产资源保护:海洋与淡水生态系统》2020,30(8):1702-1710
- Bottlenose dolphins encountered around the Irish coast are considered part of a wide‐ranging coastal community; however, knowledge on the significance of the north of Ireland for this species is limited by a lack of dedicated effort.
- Through social media, the opportunity now exists to gather large volumes of citizen science data in the form of high‐quality images, potentially extending the spatial and temporal scope of photo‐identification studies.
- The purpose of this study was to investigate social media as a data resource for photo‐identification studies and to provide a preliminary assessment of bottlenose dolphins in the north of Ireland. Specifically, the study sought to examine the photo‐identification data for spatial clustering.
- The study identified 54 well‐marked individuals and provided evidence of potential year‐round occurrence, with successful re‐sightings throughout the study period (2007–2016). There was a geographic concentration of re‐sightings along the north of Ireland, suggestive of interannual site fidelity. These results provide scientific rationale for strategically targeting the north of Ireland in future research on the Irish coastal community.
- For effective conservation of the bottlenose dolphin it is imperative that scientific research, and resultant management objectives, consider wide‐ranging communities such as the Irish coastal community. Our research highlights data collection via social media as a cost‐effective and scientifically valuable tool in the photo‐identification of coastal cetaceans. We recommend that this method is used in research on low‐density and wide‐ranging coastal cetaceans.
57.
Lynsey Macintyre Tong Zhang Christina Viegelmann Ignacio Juarez Martinez Cheng Cheng Catherine Dowdells Usama Ramadan Abdelmohsen Christine Gernert Ute Hentschel RuAngelie Edrada-Ebel 《Marine drugs》2014,12(6):3416-3448
Marine invertebrate-associated symbiotic bacteria produce a plethora of novel secondary metabolites which may be structurally unique with interesting pharmacological properties. Selection of strains usually relies on literature searching, genetic screening and bioactivity results, often without considering the chemical novelty and abundance of secondary metabolites being produced by the microorganism until the time-consuming bioassay-guided isolation stages. To fast track the selection process, metabolomic tools were used to aid strain selection by investigating differences in the chemical profiles of 77 bacterial extracts isolated from cold water marine invertebrates from Orkney, Scotland using liquid chromatography-high resolution mass spectrometry (LC-HRMS) and nuclear magnetic resonance (NMR) spectroscopy. Following mass spectrometric analysis and dereplication using an Excel macro developed in-house, principal component analysis (PCA) was employed to differentiate the bacterial strains based on their chemical profiles. NMR 1H and correlation spectroscopy (COSY) were also employed to obtain a chemical fingerprint of each bacterial strain and to confirm the presence of functional groups and spin systems. These results were then combined with taxonomic identification and bioassay screening data to identify three bacterial strains, namely Bacillus sp. 4117, Rhodococcus sp. ZS402 and Vibrio splendidus strain LGP32, to prioritize for scale-up based on their chemically interesting secondary metabolomes, established through dereplication and interesting bioactivities, determined from bioassay screening. 相似文献
58.
Using behavioral landscape ecology to predict species’ responses to land-use and climate change 总被引:1,自引:0,他引:1
Climate change and habitat destruction are widely recognized as major threats to species’ survival. As a result of these anthropogenic impacts, species are often forced into novel landscapes where their persistence is difficult to predict. Knowledge of how individuals move or disperse through the landscape, choose habitat in which to settle, and produce offspring which survive to repeat the process can greatly improve our ability to predict species’ persistence. The field of behavioral landscape ecology uses a strong theoretical base to explore, often experimentally, how the behavior of a particular species is affected by heterogeneous and rapidly changing landscapes and can offer valuable insight for managing species in the face of human-induced environmental changes. When interpreted by modelers, results of landscape-level behavioral experiments can be quantified for use in predictive models. To this end, we summarize the methods and results of research using direct experimental manipulation techniques broken into the following categories: translocations, playback experiments, food resource manipulations, manipulations of reproductive success, direct manipulations of the landscape, and manipulations of predation risk. We review and place in a theoretical framework the results from this emerging body of research regarding how organisms move in and respond to different types of landscapes, both natural and human-altered. We go onto highlight the potential of each experimental method to quantify different processes, which may be useful when interpreted by modelers attempting to parameterize predictive models. Finally, we suggest future directions for experimental research that will allow for greater integration of behavioral landscape ecology and predictive modeling. 相似文献
59.
Catherine M Shilton Margret S Thompson René Meisner Brad Lock William A Lindsay 《Journal of zoo and wildlife medicine》2002,33(4):371-377
An 18- to 25-yr-old intact female Bengal tiger (Panthera tigris) was diagnosed antemortem and postmortem with nasopharyngeal myxosarcoma metastatic to the lung, chronic lymphoplasmacytic otitis media, and lymphoplasmacytic and eosinophilic stomatitis. Myxosarcomas are rare in domestic animals and seldom metastasize; this tumor has not been previously reported in an exotic felid. Computed tomography of the skull was used during the diagnoses. 相似文献
60.
Bonnefont-Rebeix C Marchal T Bernaud J Pin JJ Leroux C Lebecque S Chabanne L Rigal D 《Veterinary immunology and immunopathology》2007,118(1-2):134-139
Toll-like receptors (TLRs) are a family of functionally important receptors for recognition of pathogen-associated molecular pattern (PAMP) since they trigger the pro-inflammatory response and upregulation of costimulatory molecules, linking the rapid innate response to adaptative immunity. In human leukocytes, TLR3 has been found to be specifically expressed in dendritic cells (DC). This study examined the expression of TLR3 in canine monocytes-derived DC (cMo-DC) and PBMC using three new anti-TLR3 mAbs (619F7, 722E2 and 713E4 clones). The non-adherent cMo-DC generated after culture in canine IL-4 plus canine GM-CSF were labelled with the three anti-TLR3 clones by flow cytometry, with a strong expression shown for 619F7 and 722E2 clones. By contrast, TLR3 expression was low to moderate in canine monocytes and lymphocytes. These results were confirmed by Western blot using 619F7 and 722E2 clones and several polypeptide bands were observed, suggesting a possible cleavage of TLR3 molecule or different glycosylation states. In addition, TLR3 was detectable in immunocytochemistry by using 722E2 clone. In conclusion, this first approach to study canine TLR3 protein expression shows that three anti-TLR3 clones detect canine TLR3 and can be used to better characterize canine DC and the immune system of dogs. 相似文献