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Genomic Sequence and Experimental Tractability of a New Decapod Shrimp Model,Neocaridina denticulata
Nathan J. Kenny Yung Wa Sin Xin Shen Qu Zhe Wei Wang Ting Fung Chan Stephen S. Tobe Sebastian M. Shimeld Ka Hou Chu Jerome H. L. Hui 《Marine drugs》2014,12(3):1419-1437
The speciose Crustacea is the largest subphylum of arthropods on the planet after the Insecta. To date, however, the only publically available sequenced crustacean genome is that of the water flea, Daphnia pulex, a member of the Branchiopoda. While Daphnia is a well-established ecotoxicological model, previous study showed that one-third of genes contained in its genome are lineage-specific and could not be identified in any other metazoan genomes. To better understand the genomic evolution of crustaceans and arthropods, we have sequenced the genome of a novel shrimp model, Neocaridina denticulata, and tested its experimental malleability. A library of 170-bp nominal fragment size was constructed from DNA of a starved single adult and sequenced using the Illumina HiSeq2000 platform. Core eukaryotic genes, the mitochondrial genome, developmental patterning genes (such as Hox) and microRNA processing pathway genes are all present in this animal, suggesting it has not undergone massive genomic loss. Comparison with the published genome of Daphnia pulex has allowed us to reveal 3750 genes that are indeed specific to the lineage containing malacostracans and branchiopods, rather than Daphnia-specific (E-value: 10−6). We also show the experimental tractability of N. denticulata, which, together with the genomic resources presented here, make it an ideal model for a wide range of further aquacultural, developmental, ecotoxicological, food safety, genetic, hormonal, physiological and reproductive research, allowing better understanding of the evolution of crustaceans and other arthropods. 相似文献
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Water, Air, &; Soil Pollution - Lake E?irdir is one of the most important freshwater resources in Turkey. The lake is located at the border of Isparta province in South West Turkey, an area... 相似文献
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The damage caused by Thrips tabaci to a susceptible hybrid of early white cabbage was studied in field experiments undertaken during 2004 and 2005. The three treatments tested were beds of cabbage mulched with: (1) barley straw, (2) hay, and (3) black polyethylene (PE) sheeting. Bare-soil (un-mulched) beds were used as the control treatment. One early and one late experiment were performed in both years. In the early experiments, the mulches were applied at the start of the experiment, whereas in the late experiments the soil was first covered with black polyethylene for approximately 1 month. In both years, the springs were cold and wet, and so the thrips appeared late and their numbers were low. The highest mean actual weight and trimmed weight were recorded for cabbage grown in 2005 under PE mulch (actual early: 995 g, actual late: 1184 g, trimmed early: 870 g, trimmed late: 1056 g). Year 2004 gave lower values (110g for both variables) under barley straw and hay mulch (165 g and 160 g, respectively). There was no evidence of an allelopathic effect of water extracts of either barley straw or hay. 相似文献
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Assessment of the production and dispersal of faecal waste from the sea‐cage aquaculture of spiny lobsters 下载免费PDF全文
Despite the rapid expansion of spiny lobster aquaculture, very little is known about its potential environmental impacts. In an effort to understand the potential impacts of sea‐cage culture of spiny lobsters, the faecal output and settling velocity of faeces of spiny lobster were quantified. These data were then used in hydrodynamic modelling to assess the dispersal pattern of faecal material from two sizes of hypothetical spiny lobster farms, so that inferences about likely benthic impacts could be made by modelling different scenarios of farm stocking density and food conversion efficiency. The overall mean settling velocity of lobster faecal strands was 0.9 ± 0.05 cm s?1. There was a positive correlation between both the weight and density of faecal strands and their settling velocity. Conversely, neither the length nor the surface area of faecal strands was correlated with the settling velocity. Hydrodynamic modelling showed that faecal material deposition mainly occurred directly beneath the sea‐cages. The density of faecal material deposition increased proportionately with higher stocking density and FCR values. Overall, the results indicate that the deposition of faecal waste from sea‐cage lobster aquaculture is unlikely to cause widespread benthic impacts when operating to the current highest commercial stocking and feeding levels. 相似文献
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Cholesterol-lowering nutraceuticals and functional foods 总被引:2,自引:0,他引:2
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OBJECTIVE: To estimate the proportion of snack food and beverage choices available to an Australian consumer. DESIGN: A survey of product Nutrition Information Panels (NIP) and product labels on snack foods and beverages offered for sale. Data on nutrient content were compared with criteria from different nutrient profile systems to estimate the proportion of items conforming to a choice. SETTING: A large supermarket in metropolitan Melbourne, Australia. RESULTS: A consumer could choose from 1070 different snack foods and 863 different drinks. Flavour variety was more common in snacks (maximum thirteen per product) while variation in container size was more common for drinks (up to ten per product). Recommended serving size for snacks varied greatly (1822 % of snack foods presented for sale could be deemed by multiple criteria. Similarly, only 14healthyhealthier' snack foods and beverages, e.g. by reformulation of many products by the food industry and their presentation in smaller, standardised portion-size packaging. 相似文献
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