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771.
Ruhl S Casson N Kaiser C Thoma R Pospischil A Greub G Borel N 《Veterinary microbiology》2009,135(1-2):169-174
Bovine abortion of unknown infectious aetiology still remains a major economic problem. In this study, we focused on a new possible abortigenic agent called Parachlamydia acanthamoebae. Retrospective samples (n=235) taken from late-term abortions in cattle were investigated by real-time diagnostic PCR for Chlamydiaceae and Parachlamydia spp., respectively. Histological sections of cases positive by real-time PCR for any Chlamydia-related agent were further examined by immunohistochemistry using specific antibodies. Chlamydophila abortus was detected only in three cases (1.3%) by real-time PCR and ArrayTube Microarray playing a less important role in bovine abortion compared to the situation in small ruminants in Switzerland. By real-time PCR as many as 43 of 235 (18.3%) cases turned out to be positive for Parachlamydia. The presence of Parachlamydia within placental lesions was confirmed in 35 cases (81.4%) by immunohistochemistry. The main histopathological feature in parachlamydial abortion was purulent to necrotizing placentitis (25/43). Parachlamydia should be considered as a new abortigenic agent in Swiss cattle. Since Parachlamydia may be involved in lower respiratory tract infections in humans, bovine abortion material should be handled with care given the possible zoonotic risk. 相似文献
772.
Eyad Atalah Carmen María Hernández‐Cruz Tibiabin Benítez‐Santana Rachid Ganga Javier Roo Marisol Izquierdo 《Aquaculture Research》2011,42(9):1279-1288
The effect of different arachidonic acid (ARA) dietary contents at several dietary eicosapentaenoic acid (EPA) levels on the growth, survival and biochemical composition of gilthead seabream larvae was studied to better define the importance of this fatty acid as a function of EPA. Larvae of 18 days were fed one of the five isonitrogenous and isolipidic microdiets with three different EPA (0.3%, 2% and 4%) and ARA amounts (0.1%, 0.6% and 1.2%). Although a dietary increase in either ARA or EPA alone did not improve survival significantly, the increase in both fatty acids significantly enhanced growth and survival, suggesting an optimum dietary value of EPA:ARA close to 4:1.2. Dietary ARA was more efficiently incorporated into larval tissues than EPA. Increased dietary EPA or ARA contents reduced the incorporation of ARA or EPA into larval lipids, indicating their competition as substrates for different enzymes. The possible negative effect of further elevation of dietary ARA and its competition with EPA for phospholipids synthesis deserves further studies in marine fish larvae. 相似文献
773.
Manuel Vargas‐Yez Ana Girldez Pedro Torres María Gonzlez Maria del Carmen García‐Martínez Francina Moya 《Fisheries Oceanography》2020,29(5):367-380
Time series of European sardine (Sardina pilchardus) landings from 1962 and environmental variables from 1978 in the northern Alboran Sea are analysed. European sardine spawns in the northern Alboran Sea from mid‐autumn to late winter at a temperature range slightly higher than the one observed in the nearby Eastern North Atlantic and the North Western Mediterranean. Individuals hatched during autumn and winter are incorporated to the fishery during the following summer and autumn producing the maximum annual landings. These landings show both a decreasing long‐term trend and a strong inter‐annual variability. Although further research is needed, the warming trend of sea surface temperature and the decrease in upwelling intensity inferred from empirical orthogonal function (EOF) analyses could have some influence on the negative trends of sardine landings. The inter‐annual variability of sardine abundance seems to be related to the wind intensity at a local scale, the second principal component of the chlorophyll concentration and the sardine abundance during the preceding year. If the inter‐annual variability is considered, a linear model including these three variables with a one‐year time lag allows to explain 79% of the sardine landings variance. If the negative linear trend is also considered, the model explains 86% of the variance. These results indicate that the body condition of spawners, linked to the food availability during the preceding year, is the main factor controlling the recruitment success. The possibility of predicting sardine landings 1 year in advance could have important implications for fishery management. 相似文献
774.
José A Orden Carmen Cortés José A Ruiz-Santa-quiteria Susana Martínez Ricardo de la Fuente 《Journal of veterinary diagnostic investigation》2005,17(1):65-67
A total of 163 verotoxin-producing Escherichia coli (VTEC) strains isolated from diarrheic and healthy cattle, sheep, and goats were analyzed for the presence of the saa gene by polymerase chain reaction. Seventeen (45.9%) and 5 (29.4%) of the VTEC isolated from healthy cattle and diarrheic calves, respectively, had the saa gene. None of the saa-positive strains carried the eae gene, but 20 of the 22 saa positive were ehxA positive. In contrast with cattle VTEC, none of the VTEC isolated from small ruminants were saa positive. These results show that the saa gene is commonly associated with bovine eae-negative VTEC strains but not with ovine or caprine VTEC strains. 相似文献