全文获取类型
收费全文 | 3248篇 |
免费 | 225篇 |
国内免费 | 1篇 |
专业分类
林业 | 259篇 |
农学 | 161篇 |
基础科学 | 17篇 |
789篇 | |
综合类 | 189篇 |
农作物 | 205篇 |
水产渔业 | 456篇 |
畜牧兽医 | 1107篇 |
园艺 | 75篇 |
植物保护 | 216篇 |
出版年
2024年 | 3篇 |
2023年 | 42篇 |
2022年 | 73篇 |
2021年 | 142篇 |
2020年 | 136篇 |
2019年 | 160篇 |
2018年 | 155篇 |
2017年 | 144篇 |
2016年 | 131篇 |
2015年 | 97篇 |
2014年 | 158篇 |
2013年 | 189篇 |
2012年 | 244篇 |
2011年 | 273篇 |
2010年 | 146篇 |
2009年 | 160篇 |
2008年 | 192篇 |
2007年 | 174篇 |
2006年 | 166篇 |
2005年 | 148篇 |
2004年 | 127篇 |
2003年 | 141篇 |
2002年 | 97篇 |
2001年 | 23篇 |
2000年 | 21篇 |
1999年 | 18篇 |
1998年 | 16篇 |
1997年 | 11篇 |
1996年 | 6篇 |
1995年 | 11篇 |
1994年 | 6篇 |
1993年 | 10篇 |
1992年 | 5篇 |
1990年 | 3篇 |
1989年 | 4篇 |
1988年 | 2篇 |
1986年 | 2篇 |
1985年 | 8篇 |
1984年 | 3篇 |
1983年 | 4篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1980年 | 4篇 |
1977年 | 2篇 |
1976年 | 3篇 |
1975年 | 1篇 |
1972年 | 1篇 |
1971年 | 3篇 |
1968年 | 1篇 |
1964年 | 1篇 |
排序方式: 共有3474条查询结果,搜索用时 390 毫秒
871.
Maria Lúcia Calijuri Eduardo de Aguiar do Couto Aníbal da Fonseca Santiago Rodrigo de Arruda Camargo Marcos D. F. M. e Silva 《Water, air, and soil pollution》2012,223(5):2157-2168
This paper presents a comprehensive study on the quality of surface and groundwater in an environmentally sensitive karstic watershed strongly pressed by urban growth. The objective of the study was to assess the integrated effects of human activities and natural characteristics of karstic environments on the quality of surface and groundwater using multivariate statistical techniques. Data from 18 physical, chemical, and microbiological water quality variables obtained throughout a hydrological year were analyzed using principal components analysis (PCA) and cluster analysis. The PCA was carried out individually for surface water and groundwater. Our goal was to study the behavior of the water quality variables at each of these systems, as well as to infer the importance of these variables on the dynamics of the water resources in the region. Our results for surface water showed that the 18 original variables could be reduced to five principal components which together accounted for 69% of the total variation in the data, whereas for groundwater, 70% of the total variation in the data was explained by five principal components. In order to evaluate the impacts of anthropic activities on the water quality at the monitoring locations, the cluster analysis was applied to the ten sampling points. The analysis identified five clusters, two of which are formed by points with low contamination levels and three where the anthropic interference is noticeable. The results suggest the existence of specific contamination sources in many points, including in the groundwater, and highlight the natural vulnerability of the karstic environments. 相似文献
872.
Mayela MONGE-MUOZ Segundo URQUIAGA Christoph MLLER Juan Carlos CAMBRONEROHEINRICHS Mohammad ZAMAN Cristina CHINCHILLA-SOTO Azam BORZOUEI Khadim DAWAR Carlos E. RODRGUEZ-RODRGUEZ Ana Gabriela PREZ-CASTILLO 《土壤圈》2021,31(2):303-313
In the tropics,frequent nitrogen(N)fertilization of grazing areas can potentially increase nitrous oxide(N2O)emissions.The application of nitrification inhibitors has been reported as an effective management practice for potentially reducing N loss from the soil-plant system and improving N use efficiency(NUE).The aim of this study was to determine the effect of the co-application of nitrapyrin(a nitrification inhibitor,NI)and urea in a tropical Andosol on the behavior of N and the emissions of N2O from autotrophic and heterotrophic nitrification.A greenhouse experiment was performed using a soil(pH 5.9,organic matter content 78 g kg-1,and N 5.6 g kg-1)sown with Cynodon nlemfuensis at 60%water-filled pore space to quantify total N2O emissions,N2O derived from fertilizer,soil ammonium(NH4+)and nitrate(NO3-),and NUE.The study included treatments that received deionized water only(control,NI).No significant differences were observed in soil NH4+content between the UR and UR+NI treatments,probably because of soil mineralization and NO3-produced by heterotrophic nitrification,which is not effectively inhibited by nitrapyrin.After 56 d,N2O emissions in UR(0.51±0.12 mg N2O-N concluded that the soil organic N mineralization and heterotrophic nitrification are the main processes of NH4+and NO3-production.Additionally,it was found that N2O emissions were partially a consequence of the direct oxidation of the soil's organic N via heterotrophic nitrification coupled to denitrification.Finally,the results suggest that nitrapyrin would likely exert significant mitigation on N2O emissions only if a substantial N surplus exists in soils with high organic matter content. 相似文献
873.
von Bernuth H Picard C Jin Z Pankla R Xiao H Ku CL Chrabieh M Mustapha IB Ghandil P Camcioglu Y Vasconcelos J Sirvent N Guedes M Vitor AB Herrero-Mata MJ Aróstegui JI Rodrigo C Alsina L Ruiz-Ortiz E Juan M Fortuny C Yagüe J Antón J Pascal M Chang HH Janniere L Rose Y Garty BZ Chapel H Issekutz A Maródi L Rodriguez-Gallego C Banchereau J Abel L Li X Chaussabel D Puel A Casanova JL 《Science (New York, N.Y.)》2008,321(5889):691-696
MyD88 is a key downstream adapter for most Toll-like receptors (TLRs) and interleukin-1 receptors (IL-1Rs). MyD88 deficiency in mice leads to susceptibility to a broad range of pathogens in experimental settings of infection. We describe a distinct situation in a natural setting of human infection. Nine children with autosomal recessive MyD88 deficiency suffered from life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease. However, these patients were otherwise healthy, with normal resistance to other microbes. Their clinical status improved with age, but not due to any cellular leakiness in MyD88 deficiency. The MyD88-dependent TLRs and IL-1Rs are therefore essential for protective immunity to a small number of pyogenic bacteria, but redundant for host defense to most natural infections. 相似文献
874.
Bruno Araujo Furtado de Mendonça Elpídio Inácio Fernandes Filho Carlos Ernesto Gonçalves Reynaud Schaefer Anôr Fiorini de Carvalho José Frutuoso do Vale Jr. Guilherme Resende Corrêa 《Journal of Soils and Sediments》2014,14(3):525-537
Purpose
The vegetation of the Campinaranas occurs in humid areas with hydromorphic sandy soils at the Amazon region. Thus, the determination and in situ monitoring of moisture content in Campinarana soils, besides the detection of subsurface layers are key measures for studying these soil–vegetation systems. Also, the application of ground penetrating radar (GPR) in deep sandy sedimentary sequence of Amazonia is a promising tool to enhance the knowledge on depositional and soil formation features.Materials and methods
We studied representative soils of the Campinaranas at the National Park of Viruá, state of Roraima (Brazilian Amazonia), through the use of geophysical methods (soil moisture sensors and GPR). The study was applied in four sandy soils. Besides chemical and physical analysis of soils, soil moisture sensors were installed for monitoring during an entire hydrological year (2010/2011), and performed the calibration of sensors , coupled with imaging of the soil along transects with GPR.Results and discussion
As a result of calibration of the soil moisture sensors we obtained a general equation with an R 2 greater than 0.9. There is an influence of soil moisture content and soil temperature in the distribution of vegetation types in Campinaranas. The use of GPR identified some determinants characteristics in these soils for the differentiating the Campinaranas, represented by spodic and C horizons.Conclusions
The spodic horizons in soils under Forest Campinarana provided potential errors in the determination of soil moisture, requiring calibration data for the precise use of this device. The investigation of the soil through the GPR showed interesting results, which allowed continuous visualization of the main soil horizons along transects in the phytophysiognomies of Campinaranas. 相似文献875.
Linearity and detection thresholds of atmospheric pressure ionization-mass spectrometry (API-MS) were determined for 11 aroma compounds in air at concentrations ranging from 50 ppb to approximately 450 ppm (moles of volatile per mole of air). In most cases, the protonated molecular ion (i.e., m/z = M + 1) was the base peak throughout the range; however, some compounds showed an increase in fragmentation at lower concentrations. Detection limits varied greatly (from 50 ppb to 14 ppm) depending upon the aroma compound being measured. The linear range was also strongly dependent upon the aroma compound, with values ranging from <10-fold change in concentration to >4000-fold change in concentration depending upon the volatile being studied. The two volatiles with poor detection thresholds also exhibited the smallest linear range. Most compounds had linear ranges of >200. There was no apparent relationship between gas-phase basicity and either detection limit or linear range. 相似文献
876.
Tudela JA Hernández JA Gil MI Espín JC 《Journal of agricultural and food chemistry》2003,51(15):4296-4302
L-galactono-gamma-lactone dehydrogenase (GLDH) activity and vitamin C content as ascorbic acid (AA) plus dehydroascorbic acid (DHA) were evaluated in five potato tuber cultivars (Agata, Altesse, Franceline, Manon, and Monalisa). The effect of fresh-cutting and subsequent refrigerated storage of Manon potato under different atmospheres (air, 20% CO(2) + air, 100% N(2), and vacuum packaging) on GLDH activity and vitamin C content was also determined. GLDH from the five potato tuber cultivars showed typical inhibition kinetics by high substrate concentration in the synthesis of AA from its physiological precursor L-galactonic acid-gamma-lactone (GL). GLDH activity was not correlated with the corresponding vitamin C content in any potato tuber cultivar. GLDH from all the cultivars presented a major isoform with isoelectric point (IEP) 5, which changed to IEP = 4.3 after minimal processing. In addition, the GLDH-catalyzed synthesis of AA by the new isoform showed typical Michaelis kinetics, in which the enzyme became more efficient to catalyze the reaction. Whether the change in the isoform pattern was due to either post-translational modifications or de novo synthesis of a new isoenzyme remains unanswered. Fresh-cutting increased GLDH activity from 4.7-fold (vacuum packaging) to 11-fold (air) after 6 days. In addition, 100% of vitamin C content was retained in air and decreased in the rest of atmospheres after this storage period, following the sequence vacuum packaging (89%) > 100% N(2) (78%) > 20% CO(2) + air (63%). This tendency was correlated with the corresponding GLDH activity detected in each storage atmosphere, except in the case of 20% CO(2) + air. Vacuum packaging proved to be the best storage condition, because fresh-cut potatoes did not turn brown and retained 89% of initial vitamin C content. 相似文献
877.
Effect of caffeic acid on the color of red wine 总被引:4,自引:0,他引:4
Darias-Martín J Martín-Luis B Carrillo-López M Lamuela-Raventós R Díaz-Romero C Boulton R 《Journal of agricultural and food chemistry》2002,50(7):2062-2067
The copigmentation effect of prefermentation additions of different doses of caffeic acid was investigated during the 1997 harvest. Microfermentation with the major red grape cultivars Listán negro and Negramoll, grown in the Canary Islands, was carried out with the same protocol. Visible and UV spectra were registered periodically. HPLC chromatograms were carried out. The color enhancement of cv. Negramoll wine varied between 13 and 75% (AU at 520 nm), and that of cv. Listán negro wine between 25 and 45% at the end of fermentation. During aging these values were enhanced to reach even >100% in some cases. An initial complex of the 1:1 type, where one molecule of caffeic acid associates with one molecule of anthocyanin, has been identified using the mathematical procedure of Brouillard et al. (J. Am. Chem. Soc. 1989, 111, 2604-2610). Caffeic acid seems to contribute to color stability and protection against oxidation. The importance of nonpigment composition in pigment extraction and color retention during and after fermentation is demonstrated. 相似文献
878.
Cantos E Tudela JA Gil MI Espín JC 《Journal of agricultural and food chemistry》2002,50(10):3015-3023
The effect of minimal processing on polyphenol oxidase (PPO), peroxidase (POD), phenylalanine ammonia-lyase (PAL), and phenolic compounds was studied in five potato cultivars (Agria, Cara, Liseta, Monalisa, and Spunta). Minimal processing caused an overall increase in PPO, POD, and PAL activities. The isoform pattern of PPO was the same for all of the cultivars before and after processing. No latent PPO was detected. The isoperoxidase pattern was approximately the same among cultivars. An increase in POD activity was related to the specific induction of an acidic isoperoxidase. PAL showed an induction pattern characterized by the presence of a maximum peak of activity after 4 days of processing for all of the cultivars. The sequence of browning susceptibility of potato cultivars was as follows: Monalisa > Spunta > Liseta > Cara > Agria. Browning development was only partially correlated to PAL activity (only during the first 4 days after wounding). However, this correlation could not explain the above sequence of browning susceptibility. Minimal processing caused an increase of chlorogenic acid, whereas tyrosine content remained unchanged. In summary, no significant correlation was found between either rate or degree of browning and any other biochemical and physiological attribute investigated (PPO, POD, hydrogen peroxide, ascorbic acid content, and initial phenolics content as well as total and individual phenolics accumulation). 相似文献
879.
Varietal differences among the polyphenol profiles of seven table grape cultivars studied by LC-DAD-MS-MS 总被引:7,自引:0,他引:7
Cantos E Espín JC Tomás-Barberán FA 《Journal of agricultural and food chemistry》2002,50(20):5691-5696
Polyphenols present in red table grape varieties Red Globe, Flame Seedless, Crimson Seedless, and Napoleon, and the white varieties Superior Seedless, Dominga, and Moscatel Italica were analyzed by HPLC-DAD-MS. The anthocyanins peonidin 3-glucoside, cyanidin 3-glucoside (and their corresponding p-coumaroyl derivatives), malvidin 3-glucoside, petunidin 3-glucoside, and delphinidin 3-glucoside were found. In addition, caffeoyltartaric acid, p-coumaroyltartaric acid, and the flavonols quercetin 3-glucuronide, quercetin 3-rutinoside, quercetin 3-glucoside, kaempferol 3-galactoside, kaempferol 3-glucoside, and isorhamnetin 3-glucoside were detected. Flavan-3-ols were also detected, and were identified as gallocatechin, procyanidin B1, procyanidin B2, procyanidin B4, procyanidin C1, catechin, and epigallocatechin. These phenolics were present only in the skin, as the flesh of these grape cultivars was almost devoid of these compounds. Anthocyanins were the main phenolics in red grapes ranging from 69 (Crimson Seedless) to 151 (Flame Seedless) mg/kg fresh weight of grapes, whereas flavan-3-ols were the most abundant phenolics in the white varieties ranging from 52 (Dominga) to 81 (Moscatel Italica) mg/kg fresh weight of grapes. Total phenolics ranged from 115 (Dominga) to 361 (Flame Seedless) mg/kg fresh weight of grapes. This means that a serving of unpeeled table grapes (200 g) could provide up to 72 mg of total phenolics (Flame Seedless). These results indicate that the intake of unpeeled table grapes should be recommended in dietary habits as a potential source of antioxidant and anticarcinogenic phenolic compounds. 相似文献
880.
Daniel Bravo-Pérez Ma. Teresa Santillán-Galicia Roberto M. Johansen-Naime Héctor González-Hernández Obdulia L. Segura-León Daniel L. Ochoa-Martínez Stephanie Guzman-Valencia 《农业科学学报》2018,17(11):2509-2517
Avocado is one of the most important crops in the world, and Mexico is the largest producer of this fruit. Several insect pests affect its production, and thrips are amongst the most important. A key step in the design of control methods is accurate species identification. Despite this, formal reports on species diversity of thrips in Mexico are very scarce. Morphological identification can sometimes be time-consuming and inconclusive. Therefore, we explored the species diversity of thrips in Mexican avocado orchards(Michoacan state) based on partial sequences of the mitochondrial gene cytochrome oxidase subunit Ⅰ(COⅠ). Forty-four specimens were analysed, which represented approximately 8% of all individuals collected from five localities distributed in three Municipalities. All specimens were analysed using the COⅠ marker, and specimens within the genera Frankliniella were also analysed using a marker within the D2 domain of the 28 S(28 SD2) nuclear ribosomal DNA. Molecular identifications were confirmed using morphological taxonomy. Overall, six genera were found(Neohydatothrips, Scirtothrips, Frankliniella, Arorathrips, Caliothrips and Leptothrips). All genera contained only one species, except Frankliniella, for which there were six species. Data from the two molecular markers suggest the existence of cryptic species within Mexican F. occidentalis populations. 相似文献