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101.
Jos�� Luis R. Gallego Carlos Sierra Albert Permanyer Ana I. Pel��ez Demelza Men��ndez-Vega Jes��s S��nchez 《Water, air, and soil pollution》2011,217(1-4):197-211
Here, we addressed biodegradation vs. volatilization processes, and also bioavailability limitations during biopile remediation of soil initially contaminated by more than 5,000 mg/kg of hydrocarbons. In order to select bioremediation strategies, we first conducted a biotreatability study, which included geochemical, textural, and microbiological characterization of the soil matrix. Next, we implemented five bioremediation approaches onsite in real-scale biopiles. In order to monitor hydrocarbon depletion and to distinguish between biological and non-biological processes, we analyzed chemical biomarkers by means of gas chromatography?Cmass spectrometry. In addition, a comprehensive study of soil grain size and its implications on bioavailability were studied. Furthermore, the evolution of microbial populations was also examined. Two of the strategies implemented in the biopiles (the combination of a slow-release fertilizer and a surfactant, and the use of an oleophilic fertilizer respectively) reduced the soil hydrocarbon content to under 500 mg/kg in 5 months. Additional results from this study indicate that volatilization was the predominant degradation process for light hydrocarbons (below 12 carbon atoms), whereas heavier compounds were mainly biodegraded. However, even in the most favorable situation, a residual concentration of hydrocarbons linked to the finer fraction of the soil was found. 相似文献
102.
Interactions with food components may alter the resistance of food proteins to digestion, a property thought to play an important role in determining allergenic properties. The kinetics of breakdown of the bovine milk allergen alpha-lactalbumin during in vitro gastrointestinal digestion was found to be altered by interactions with physiologically relevant levels of phosphatidylcholine (PC), a surfactant that is abundant both in milk and is actively secreted by the stomach. Breakdown during gastric digestion was slowed in the presence of PC and accompanied by small alterations in the profile of resulting peptides, with little effect being observed during subsequent duodenal digestion. alpha-Lactalbumin was found to unfold at gastric (acid) pH, giving a CD spectrum similar to that obtained for the partially folded state it is known to adopt at pH values below its isoelectric point. Fluorescence polarization studies performed at low pH indicated that this partially unfolded form of the protein was able to penetrate into the PC vesicles. These interactions are probably responsible for the slowing of gastric digestion by reducing the accessibility of the protein to pepsin. These findings show that interactions with other food components, such as lipids, may alter the rate of breakdown of food proteins in the gastrointestinal tract. It underlines the importance of the food matrix in affecting patterns of food allergen digestion and hence presentation to the immune system and that in vitro digestion systems used for assessing digestibility of allergens must take account of surfactants. 相似文献
103.
Africa Sánchez Carlos Cadahía Enrique Eymar Alberto Masaguer 《Journal of plant nutrition》2013,36(7):1007-1024
Nitrogen (N) optimization of a nutrient solution for ornamental conifers was obtained with two different experiments in hydroponic cultures: First, by using three different NO3 ‐/NH4 + ratios (60/40, 55/45, and 40/60 in percentages of the total N supplied); and, secondly by testing three total N levels (3.7; 4.7 and 5.5 mmol L‐1). Best growth was obtained with a NO3 ‐/NH4 + ratio of 55/ 45 and a total N level of 3.7 mmol L‐1. With these experiments, reference levels of foliar concentrations of the macronutrients N, phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), and the biochemical indices, such as chlorophyll and starch levels, were obtained with the treatments corresponding to the plants with the higher growth. In the course of a growth cycle, a substrate assay with two different pot mixes (moss peat plus perlite and black peat plus sepiolite 60/40 ‐% v/v‐) was carried out by using the best N ratios and doses in nutrient solutions as obtained in hydroponics. The results indicated that the same N fertilization in fertigation systems changes depending on the different physicochemical properties of the substrates used; in this case, depending on the different physical properties of the two substrates. By applying DRIS to perform nutritional diagnosis, it is possible to find nutritional limitations to plant growth, but not additional factors, such as water‐air relationships in the growth media. 相似文献
104.
Carlos A. Guerra Marc J. Metzger Joachim Maes Teresa Pinto-Correia 《Landscape Ecology》2016,31(2):271-290
Context
Policy decisions form a major driver of land use change, with important implications for socially and environmentally susceptible regions. It is well known that there can be major unintended consequences, especially where policies are not tailored to regionally specific contexts.Objectives
In this paper we assess the implications of 60 years of agricultural policies on soil erosion prevention (SEP) by vegetation, an essential regulating ecosystem service in Mediterranean Europe.Methods
To assess these implications we produced and analysed a time series of land cover/use and environmental conditions datasets (from 1951 to 2012) in relation to changing agricultural policies for a specific region in the southern Portugal. A set of indicators related to SEP allowed us to identify that land use intensification as increased soil erosion in the last 60 years.Results
Particularly in the last 35 years, as a consequence of headage payments for cattle, the agricultural policy had a significant effect in the density and renewal of the tree cover, resulting in drastic effects for the provision of the SEP service. These are more significant after 1986, coinciding with the implementation of several Common Agricultural Policy instruments focused on increasing the modernization and productivity capacity of farm systems.Conclusions
The results show some unintended effects of agricultural policy mechanisms on ecosystem service provision and highlight the need for context-based policies, tailored to the environmental constrains and potentials of each region.105.
João H Perotta Paulo A Canola Maristela CS Lopes Patrícia M Évora Paulo EB Martinez André Escobar Carlos AA Valadão 《Veterinary anaesthesia and analgesia》2014,41(4):357-364
ObjectiveTo evaluate the effects of intravenous (IV) or intramuscular (IM) hyoscine premedication on physiologic variables following IV administration of medetomidine in horses.Study designRandomized, crossover experimental study.AnimalsEight healthy crossbred horses weighing 330 ± 39 kg and aged 7 ± 4 years.MethodsBaseline measurements of heart rate (HR), cardiac index (CI), respiratory rate, systemic vascular resistance (SVR), percentage of patients with second degree atrioventricular (2oAV) block, mean arterial pressure (MAP), pH, and arterial partial pressures of carbon dioxide (PaCO2) and oxygen (PaO2) were obtained 5 minutes before administration of IV hyoscine (0.14 mg kg?1; group HIV), IM hyoscine (0.3 mg kg?1; group HIM), or an equal volume of physiologic saline IV (group C). Five minutes later, medetomidine (7.5 μg kg?1) was administered IV and measurements were recorded at various time points for 130 minutes.ResultsMedetomidine induced bradycardia, 2oAV blocks and increased SVR immediately after administration, without significant changes in CI or MAP in C. Hyoscine administration induced tachycardia and hypertension, and decreased the percentage of 2oAV blocks induced by medetomidine. Peak HR and MAP were higher in HIV than HIM at 88 ± 18 beats minute?1 and 241 ± 37 mmHg versus 65 ± 16 beats minute?1 and 192 ± 38 mmHg, respectively. CI was increased significantly in HIV (p ≤ 0.05). Respiratory rate decreased significantly in all groups during the recording period. pH, PaCO2 and PaO2 were not significantly changed by administration of medetomidine with or without hyoscine.Conclusion and clinical relevanceHyoscine administered IV or IM before medetomidine in horses resulted in tachycardia and hypertension under the conditions of this study. The significance of these changes, and responses to other dose rates, requires further investigation. 相似文献
106.
107.
108.
Irene Romero Carlos Fernandez Caballero María T. Sanchez-Ballesta María I. Escribano Carmen Merodio 《Postharvest Biology and Technology》2009,54(2):118-121
We have analyzed the influence of the stage of ripeness on L-phenylalanine ammonia-lyase (PAL) gene expression, accumulation of anthocyanins and total phenolics, and on antioxidant activity in the skin of table grapes treated with 20% CO2 + 20% O2 + 60 % N2 for 3 or 6 d at low temperature (0 °C). The residual effect of high CO2 treatment after transfer to air was also studied. In early harvested grapes, neither the anthocyanin content nor the accumulation of VcPAL mRNA was affected by any of the CO2 treatments applied. However, in late harvested grapes, the duration of high CO2 treatment determined its effect and a 6 d treatment with CO2 sustained higher levels of total phenolics and anthocyanin accumulation, and VcPAL expression than observed in untreated late harvested grapes. The increased antioxidant capacity was correlated with the total amount of phenolics and anthocyanins. Conversely, in grapes treated for 3 d with CO2 the phenylpropanoid pathway did not appear to be induced and a relationship between antioxidant activity and anthocyanins was not observed. Thus, further studies are needed to identify the most important antioxidants in these treated fruit. 相似文献
109.
Pérez-Brígido Carlos D. Romero-Salas Dora Pardío-Sedas Violeta T. Cruz-Romero Anabel González-Hernández Milagros Delprá-Cachulo Joyce Mara Ascencio Mariano Florin-Christensen Mónica Schnittger Leonhard Rodríguez Anabel E. 《Veterinary research communications》2022,46(1):295-302
Veterinary Research Communications - The black-handed spider monkey (Ateles geoffroyi) is a platyrrhine primate distributed in southern Mexico, Central America, and part of South America. Two... 相似文献
110.
ent-Pimara-8(14),15-dien-19-oic acid (PA) isolated from Viguiera arenaria (Asteraceae; Heliantheae) inhibited rat carotid rings contraction induced by phenylephrine (10(-8) mol/l) or potassium chloride (45 mmol/l) at concentration ranging from 5 to 20 microg/ml. This inhibitory effect was not reversible after the removal of this compound from the medium bath. 相似文献