天祝县牧区开发示范工程项目草地围栏建设及其综合效益

天祝县牧区开地示范工程项目１９９５年由农业部和国家计委审批立项,１９９６年全面启动建设,该项目以良种繁育,疫病防治,草地建设,技术服务四大体系为主体建设内容,并于１９９８年８月通过验收,围栏是草地建设体系的主要建设内容,在突出生产性,公益性,基础性建设原则的基础上,自１９９６年以来累计建成４０００ｈｍ＾２建成后,经综合改良,使草地植被得以迅速恢复,平均增产鲜草１６５３ｋｇ／ｈｍ＾２,年创经济效益３     

氟对牛脾淋巴细胞膜ATP酶活性的影响

取健康牛脾脏,常规方法分离培养淋巴细胞,用不同浓度氟化钠（NaF）染毒24h。低渗溶胞法提取细胞膜,检测膜上ATP酶活性。结果显示,与对照组相比较,染氟组淋巴细胞膜ATP酶活性降低,组间差异显著或极显著。表明：氟能抑制牛脾淋巴细胞膜ATP酶活性。     

小尾寒羊产后子宫内细菌类型和数量的研究

通过子宫内膜活检采样器得到子宫腔内液,应用细菌分离培养鉴定技术,研究10只小尾寒羊产后不同时期子宫内细菌的种类和数量的变化规律。结果发现:在产后1~25 d,小尾寒羊子宫腔内存在4个属的细菌,这些细菌是以芽孢杆菌属、葡萄球菌属、微球菌属为主的革兰氏阳性菌和以大肠杆菌群为主的革兰氏阴性菌等。产后子宫细菌数量和种类随着子宫内环境的改变而有规律地变化:从无菌状态增加到最高点约7 d左右,而下降比较缓慢,到子宫颈管复旧完成(22 d)后,才接近于无菌状态。产后子宫内一定数量的细菌对子宫复旧无显著影响。     

Effects of growth factors (EGF,PDGF-BB and TGF-beta 1) on cultured equine epithelial cells and keratocytes: implications for wound healing

Objective The physiologic mechanisms involving growth factors, including PDGF‐BB, EGF, and TGF‐β1, as potent mediators of fibroblasts and epithelial cells in corneal wound healing remain unknown. The goal of this study was to determine culture methods for equine epithelial cells and keratocytes and to investigate how exogenous growth factors influence proliferation of both cell types. Procedures Cell cultures were established from healthy corneas harvested from horses immediately following euthanasia and maintained using standard tissue culture protocols. To determine the effects of PDGF‐BB, EGF, TGF‐β1, keratocytes (1 × 10⁵/well) and epithelial cells (2 × 10⁵/well) were each cultured in 12 well plates and exposed separately to the growth factors. The cells were exposed to concentrations of EGF between 0 and 50 ng/mL; PDGF‐BB between 0 and 75 ng/mL; and TGF‐β1 between 0 and 10 ng/mL. Cell proliferation was measured using ³H‐thymidine assay and differences in growth determined using anova and Tukey's HSD test (P < 0.05). Results Epithelial cell and keratocyte cultures were successfully established. EGF maximally stimulated keratocyte and epithelial cells at 25 ng/mL and 5 ng/mL, respectively. PDGF‐BB maximally stimulated keratocytes and epithelial cells at 50 ng/mL and 5 ng/mL, respectively. TGF‐β1 inhibited keratocytes at 5 ng/mL and 10 ng/mL, and epithelial cells at 1 ng/mL and 2 ng/mL. Conclusions Methods were established to maintain epithelial cells and keratocytes in vitro. PDGF‐BB and EGF stimulate, while TGF‐β1 inhibits the proliferation of epithelial cells and keratocytes. These growth factors may play a role in maintenance and repair of the equine cornea.     

一例种鸡维生素B2缺乏症的诊治

2003年4月中旬,济宁某种鸡场连续孵化3批种蛋,孵化期间均发生大批胚胎死亡,出壳的雏鸡表现弱雏,成活率极低。经询问种鸡饲养状况和观察死亡胚、死亡雏鸡的症状,确诊为种鸡维生素B2缺乏症。送检的死亡胚胎和1日龄死亡雏鸡症状基本相似。胚胎多在孵化后12-14天死亡,死胚颈部弯曲,足肢缩短,关节变形,水肿,比相同日龄的正常发育胚要小。死雏的特征性症状非常明显,即所有的雏鸡均无绒毛,只在胸部、背部、颈部等部位无规则地出现疏密不一的棒状羽,短而粗,有的羽毛紧贴于皮肤呈结节状。多只死雏表现典型的“鹦鹉嘴”,嘴尖而短,喙部向里侧弯曲,使得…     

静注克洛素隆在绵羊体内的药代动力学研究

应用YWG-C18色谱柱(250 mm×4.6 mm,5μm),WatersTM 486型可调波长紫外检测器(检测波长265 nm);0.01 M磷酸钾(pH=7)乙腈(3 1)为流动相;含量测定采用标准曲线法,建立了RP-HPLC法检测绵羊血浆中克洛素隆含量的方法.同时对绵羊单剂量静脉注射7 mg/kg克洛素隆的药代动力学进行了研究.血药浓度在0.005～2.0 μg/ml及2.0～50.0μg/ml范围呈良好线性关系(r=0.9941、0.9970),平均回收率93.2%,血药最低检测浓度0.005μg/ml,日内日间变异系数分别小于10%、11%.血药浓度结果经MCPKP药代动力学统计软件处理,体内药物运转符合三室开放模型,主要药物动力学参数t1/2γ=14.14 h,Kel=0.3260 h-1,AUC=153.95 mg/l@h,fc=0.055.结果表明克洛素隆静脉单次给药后体内分布较为广泛,持续作用时间长,主要通过肾脏排泄.     

复合微生态制剂对蛋种鸡小肠黏膜双糖酶活性的影响

为研究日粮中添加复合微生态制剂对蛋种鸡小肠黏膜双糖酶活性的影响,试验选用1日龄海兰褐蛋种鸡320只,随机分为4个组,试验1、2、3组分别在空白对照组日粮基础上添加50 g/t、100 g/t、200 g/t的复合微生态制剂,分别测定21、42和112日龄蛋种鸡十二指肠、空肠和回肠黏膜中蔗糖酶、乳糖酶和麦芽糖酶的活性。结果显示:①在21和42日龄时,试验2组蛋种鸡十二指肠和空肠黏膜的蔗糖酶活性显著高于对照组(P<0.05);②21和42日龄时,试验组蛋种鸡十二指肠、空肠和回肠黏膜的乳糖酶活性显著高于对照组(P<0.05);③21日龄时,与对照组相比,试验2、3组蛋种鸡空肠黏膜的麦芽糖酶活性显著提高(P<0.05);④42和112日龄时,试验2组空肠黏膜的麦芽糖酶活性都高于对照组(P<0.05)。结果提示日粮中添加复合微生态制剂可提高蛋种鸡小肠黏膜双糖酶活性,在本试验条件下,100 g/t为最适的添加剂量。     

某猪场支气管败血波氏杆菌和化脓隐秘杆菌混合感染的病原分离鉴定及药敏试验

支气管败血波氏杆菌是引起猪萎缩性鼻炎的病原之一,化脓隐秘杆菌是一种感染牛、羊、猪等动物的机会致病菌.国内目前没有关于二者混合感染猪的报道.本研究从陕西省某猪场发病仔猪肺脏中分离出两株病原菌,经染色镜检、生化试验和16S rDNA测序鉴定,确定病原菌为支气管败血波氏杆菌和化脓隐秘杆菌.采用K-B纸片扩散法进行药敏试验,发...     

口饲胰岛素样生长因子的生理功能

本文综述了口饲胰岛素样生长因子的动物性实验结果。口饲胰岛素样生长因子具有降低血糖水平、增强机体免疫力、调节动物肠道菌群,促进胃肠道生长发育或肠道组织创伤的愈合等独特的生理功能。