Alteration of apoptotic susceptibility and bcl-2 gene in malignant transformation of human bronchial epithelial cells

AIM: To demonstrate the susceptibility of cell apoptosis varies during the progress of cell malignant transformation from human being in vitro. METHODS: A SV₄₀T-transfected human bronchial epithelial immortalized cell line (called M) was selected in this work, which has acquired some characteristics of malignant transformation at the later passage. The alterations of apoptosis and bcl-2, P53 genes between early and later passage of M cells were investigated by means of TDT labeling in situ, chromosome FISH, RNA and protein testing, etc. RESULTS: Incidence of apoptosis induced by cis-platin was significantly lower in later than in early passages of M. Levels of bcl-2 mRNA and protein in later passages were higher than early passages of M, and overexpression of bcl-2 was accumulated following the development of cellular malignancy. P53 protein level was as high in early as in later passages. CONCLUSION: Overexpression of bcl-2 decreases the cellular sensitivity to apoptotic inductors plays an important role during progress of carcinogenesis in human bronchial epithelial cancers. The inactivation of P53 protein in the SV₄₀-T transfected M cell line may be one of reasons of bcl-2 overexpression, but not associated with the accumulation of bcl-2 expressed level during cell transformation.     

Inducible nitric oxide synthase and arterial pressure regulation

AIM and METHODS:To clarify the role of inducible nitric oxide synthase (iNOS) in the regulation of blood pressure,in the present study, we examined the effect of aminoguanidine (AG), a selective inhibitor of iNOS on the hemodinamical response of Dahl salt-sensitive (DS) and Dahl salt-resistant (DR) rats to low (0.3%) or high (8%) sodium chloride (NaCl) infusion by chronical in vivo hemodynamic experiment, and the effect of NaCl or NaCl plus AG infusion on urinary nitrate (NO₃)/nitrite (NO₂), the end product of nitric oxide (NO), excretion by Greiss Reaction. Furthermore, NOS activity assay was also carried out to probe the effect of NaCl and AG on calcium-dependent or independent NOS activity in renal tissue. RESULTS:1. High or low NaCl-infused DR rats and low NaCl-infused DS rats have no hemodinamical response to AG, however, the hypertensive effect of high NaCl (8%) infusion on DS rats were greatly amplified by co-infusion of AG. 2. Administration of high NaCl significantly elevated the iNOS activity of renal tissue, and greatly increased urinary NO₃/NO₂ excretion. CONCLUSION:Inducible NOS is an important modulator of arterial pressure, especially in case of higher blood pressure.     

近红外光谱分析法测定菜籽油中芥酸的含量

采用多通道PDA型近红外光谱仪,应用偏最小二乘法建立了菜籽油中芥酸含量与近红外透射光谱的校正模型,讨论多项式求导及平滑的窗口宽度和相关系数法筛选有效波长对校正模型的影响,并对10个预测集样品利用预测相关系数R_p和预测均方根误差RMSEP指标进行了预测精度分析,结果发现：在使用全谱数据进行偏最小二乘回归建模时,一阶7点求导及平滑的预处理方法结果最佳,此时建模效果为：R_p=0.739,RMSEP=1.659;在此基础上通过相关系数法筛选波长后的建模效果为：R_p=0.958,RMSEP=0.963。后者R_p提高29%,RMSEP减少42%。由此可得出多项式求导及平滑法和相关系数法相结合对校正模型稳健性,预测精度都有较大提高的结论。研究证明：多通道近红外光谱仪快速测测菜籽油中芥酸含量的方法是可行的。     

馆藏书目数据优化

在实际工作中,常常因为各种原因致使馆藏书目数据的有效性和唯一性大打折扣。作者主要是通过自编程序来实现馆藏书目数据优化,并详细介绍了具体的操作过程。     

Comparison of Physicochemical and Structural Properties of Acid-Soluble and Pepsin-Soluble Collagens from Blacktip Reef Shark Skin

Fish collagen has been widely used in tissue engineering (TE) applications as an implant, which is generally transplanted into target tissue with stem cells for better regeneration ability. In this case, the success rate of this research depends on the fundamental components of fish collagen such as amino acid composition, structural and rheological properties. Therefore, researchers have been trying to find an innovative raw material from marine origins for tissue engineering applications. Based on this concept, collagens such as acid-soluble (ASC) and pepsin-soluble (PSC) were extracted from a new type of cartilaginous fish, the blacktip reef shark, for the first time, and were further investigated for physicochemical, protein pattern, microstructural and peptide mapping. The study results confirmed that the extracted collagens resemble the protein pattern of type-I collagen comprising the α₁, α₂, β and γ chains. The hydrophobic amino acids were dominant in both collagens with glycine and hydroxyproline as major amino acids. From the FTIR spectra, α helix (27.72 and 26.32%), β-sheet (22.24 and 23.35%), β-turn (21.34 and 22.08%), triple helix (14.11 and 14.13%) and random coil (14.59 and 14.12%) structures of ASC and PSC were confirmed, respectively. Collagens retained their triple helical and secondary structure well. Both collagens had maximum solubility at 3% NaCl and pH 4, and had absorbance maxima at 234 nm, respectively. The peptide mapping was almost similar for ASC and PSC at pH 2, generating peptides ranging from 15 to 200 kDa, with 23 kDa as a major peptide fragment. The microstructural analysis confirmed the homogenous fibrillar nature of collagens with more interconnected networks. Overall, the preset study concluded that collagen can be extracted more efficiently without disturbing the secondary structure by pepsin treatment. Therefore, the blacktip reef shark skin could serve as a potential source for collagen extraction for the pharmaceutical and biomedical applications.     

一起东佛里生奶绵羊附红细胞体病流行病学调查、诊疗报告

为探究附红细胞体在内蒙古呼和浩特市托克托县地区的流行情况,接诊呼和浩特托县地区某东佛里生奶绵羊养殖场病例,该场近一周多圈舍出现产奶羊只体温升高、精神沉郁、瘫软、食欲减退或废绝等症状,有出现症状1 ~ 2 d迅速死亡病例,也有使用抗生素硫酸庆大霉素-青霉素联用结合补液治疗1周无好转或死亡病例,死亡总数超8只,仍有10余只相似症状正在接受治疗。经现场剖检、圈舍环境调查、样品实验室检测,结合临床诊疗,确诊此次疫情为奶绵羊附红细胞体病,且周边羊养殖场（户）羊只也存在附红细胞体感染。说明附红细胞体在托克托县呈地方性流行。     

河蟹养殖及蟹文化④一龄蟹种的培育技术

当蟹苗在培育池中蜕完3次壳成为Ⅲ期仔蟹(约2万只/kg)时,即可将其转移到二级培育池中进行一龄蟹种的培育.而如果是5月中旬购入的人工蟹苗,则可培养至Ⅳ期仔蟹(约8000只/kg)再移入二级培育池培育.其目的是提高一龄蟹种(扣蟹)的成活率,保持蟹种的规格均匀,防止产生小绿蟹.     

空气悬架对提高车辆抗倾翻能力的分析

针对近年来商用车侧翻事故的凸现,提出了一种利用空气悬架提高车辆倾翻阀值,减小车辆倾翻危险的方法,编写了控制器,在MATLAB中SIMULINK下建立了七自由度车辆模型,并进行了仿真。仿真结果表明,半主动空气悬架能够有效提高车辆倾翻阀值,提高车辆抗侧翻能力。     

接种蚯蚓与添加有机物料对茶园土壤结构的影响

蚯蚓通过取食、排泄、分泌黏液、挖掘洞穴等活动,可显著改善土壤结构,提高土壤肥力.为探究蚯蚓与有机物料不同配施方式对茶园土壤肥力的调控效果与机理,设计5个处理组:不施肥(CK),不施肥+蚯蚓(BE),菜籽饼+蚯蚓(CE),茶树修剪物+蚯蚓(JE),生物质炭+蚯蚓(TE),分别进行室内模拟实验.结果显示,与不施肥(CK)相...     

汽车驾驶室人机设计CAD系统的研究

研制出一套用于汽车驾驶室人机设计CAD系统,该系统利用人机工程学辅助汽车的座椅设计、控件设计、仪表板设计、视野设计和内部空间设计。几个实例验证了系统的实用性。