炼铁乡桉树林分改造参试树种生长调查分析

根据云南省炼铁乡的自然环境条件，选择加州李、中林美荷杨、圣诞树、桤木、慈竹、香竹、梅树等7个树种进行造林试验。通过对树种主要指标的测试和数学分析，初步选择出该地区桉树林分改造的参试树种继续观测和试验。     

应用双向电泳分析2株不同毒力的副猪嗜血杆菌蛋白质表达的差异

副猪嗜血杆菌是猪常见细菌性疾病的病原之一,临床表现为多发性纤维素性浆膜炎、关节炎和脑膜炎。运用蛋白质组学技术对不同毒力的副猪嗜血杆菌(Haemophilus parasuis)进行比较研究,明确差异蛋白质信息,对揭示其致病机制具有重要意义。本试验通过应用建立的双向电泳方法对选取同为4型的2株不同H.parasuis临床分离株(菌株A和菌株B)进行蛋白质组学研究,二者相互比较,共鉴定出10个差异表达蛋白质,其中菌株A有3个蛋白质表达量为增加,分别是谷氨酰tRNA合成酶、半胱氨酸合成酶和假设蛋白;菌株B有7个蛋白质表达量为增加,包括Yae T蛋白(omp85)、CTP合酶、天冬氨酰tRNA合成酶(有3个)、尿苷激酶、SecB蛋白。差异蛋白质的功能主要集中在蛋白质合成、氨基酸合成和嘧啶合成。研究结果进一步丰富了H.parasuis的毒力因子和致病机制的相关信息,也为今后开展H.parasuis免疫蛋白质组学研究奠定了基础。     

Diagnostic Report of Clostridium chauvoei Infection of Cattle in Yanbian of Jinlin Province

The study was aimed to rapidly diagnose two ill and dead cattle in three bay town of Yanji city in Jilin province.We collected liver,spleen,heart,muscle and other tissues to diagnose Clostridium chauvoei infection by epidemiological investigation,clinical autopsy,microscopic obsevation,bacterial culture observation,biochemical experiment,molecular biology diagnosis and animal experiment.The muscles of the ill and dead cattle contacted with crepitus,and the section had a lot of blood and bubble flowing out,microscopic obsevation found bacillus with obtuse at both ends and spores,the bottom of the tube had loose and white precipitate in the broth,biochemical experiments showed that the isolate had acid-production and gas-production reactions which was unique to anaerobic bacteria causing Clostridium chauvoei infection,and 501 bp fragment was amplified by PCR.The results showed that two ill and dead cattle were casued by Clostridium chauvoei infection in Yanbian area,and proved the existence of Clostridium chauvoei.The test separated Clostridium chauvoei Yanbian strain for the frist time,which provided important reference basis for the prevention and treatment of Clostridium chauvoei infection,and laid the important foundation for the further study of drug and immune preventions of this disease.     

Shenmai injection improves insulin resistance in 3T3-L1 cells

AIM: To discuss the effect of Shenmai injection on insulin resistance (IR) in 3T3-L1 cells and its mechanisms. METHODS: 3T3-L1 preadipocytes were induced by chemical reagents to differentiate into fully differentiated adipocytes. Oil red O staining was used to detect the differentiation level of the adipocytes. The insulin-resistant 3T3-L1 cell model was demonstrated using insulin, which was confirmed by glucose concentration in cell supernatant. The IR cell model was given 10 μmol/L rosiglitazone, 25 and 50 g/L Shenmai injection and normal saline for comparison. MTT assay was used to assess the cell activity of 3T3-L1 cells which was treated with drugs for 8, 16, 24 and 36 h. Glucose oxidase method was used to detect the glucose concentration in the cell supernatant at 8, 16 and 24 h. The protein levels of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), AKT and p-AKT were determined by Western blot. RESULTS: 3T3-L1 adipocytes were successfully induced as shown by the positive oil red O staining. The IR cell model was demonstrated, and glucose concentration in the cell supernatant after treatment with Shenmai injection showed that Shenmai injection reduced the IR in 3T3-L1 cell model. The protein levels of GLUT4, PI3K and p-AKT increased compared to control group. CONCLUSION: Shenmai injection reduces the IR in 3T3-L1 cell model, which functions by increasing the protein levels of GLUT4, PI3K and p-AKT.     

硼和赤霉素对提高色买提杏座果率和果实品质的初步研究

试验在色买提杏花蕾膨大期喷施(0.1;、0.3;、0.5;)硼和盛花期喷施 (50、100和150 mg/L)赤霉素,结果表明:硼、赤霉素均可显著提高色买提杏的座果率,其中硼以0.1;的浓度最好,赤霉素以100 mg/L的浓度最好;硼、赤霉素还能明显提高色买提杏果实品质,果实可溶性固形物和硬度均与对照差异显著,赤霉素对单果重、果形指数、侧径有明显的增大趋势,而硼的作用不明显,这与它们不同的作用机理有重要的关系.     

血根碱对LPS诱导后黄鳝免疫应激及肠道炎症相关基因表达的影响

为探讨血根碱是否具有缓解黄鳝(Monopterus albus)由脂多糖(LPS)诱导后引起的免疫应激及炎症反应作用,本实验设置对照组(基础饲料)和实验组(基础饲料+750μg/kg血根碱),每组3个重复,养殖实验持续8周,结束后每个重复随机取30尾规格一致的黄鳝经腹腔注射大肠杆菌脂多糖(LPS),在注射前0 h和注射后3 h、6 h、12 h、24 h和48 h进行采样。结果显示,在0 h时,实验组肝脏中超氧化物歧化酶(SOD)活力显著高于对照组(P0.05),其余指标均无显著性差异(P0.05);血清中葡萄糖(GLU)、皮质醇(COR)、IgM、C4、碱性磷酸酶(AKP)、谷草转氨酶(GOT)、谷丙转氨酶(GPT)和肝脏中SOD在腹腔注射LPS后均呈先上升后下降的趋势,并在3 h或6 h达到峰值,肝脏中丙二醛(MDA)的变化趋势与其相反;与对照组相比,实验组血清中AKP、IgM、C4的含量和肝脏中SOD活力在注射LPS后上升幅度更大,GLU和COR的含量上升幅度慢,并且GLU、COR、AKP、IgM、C4、GOT和GPT恢复至正常水平速度更快。肠道中炎症相关基因IL-1β、IL-10、IL-12β、IL-15、TGF-β1和TGF-β2表达量在腹腔注射LPS后呈先上升后下降的趋势,并在6 h或12 h达到峰值;与对照组相比,实验组下调了IL-1β和IL-12β基因表达量,上调了IL-10、IL-15、TGF-β1、TGF-β2和TGF-β3基因表达量。综上所述,在本研究条件下,腹腔注射LPS诱导黄鳝产生了免疫应激及炎症反应,在3～6 h时出现高峰,而饲料中添加750μg/kg血根碱能够有效改善黄鳝由LPS诱导产生的免疫应激及炎症反应。     

不同基质配比对西瓜砧木嫁接前期生长的影响

西瓜嫁接常用砧木为南瓜与葫芦,本研究以草炭、蛭石、珍珠岩、椰糠为育苗基质,探究基质理化性质及不同配比对2种砧木生长的影响,以西瓜嫁接育苗常规基质配比为对照(CK,草炭∶蛭石∶珍珠岩=3∶2∶2)。试验结果表明,不同基质配比对2种砧木有明显影响,T1处理组(椰糠∶蛭石=3∶2)对2种砧木的发芽率、下胚轴长、株高、茎粗、干质量、壮苗指数等效果最好,且每盘基质成本较对照降低40%。     

Research on protoplast preparation and culture in Gladiolus hybridus Hort.

Protoplasts prepared from the aseptic leaves of Rose Supreme in Gladiolus hybridus Hort. were cultured. The yield of protoplasts with vigour was the highest under the following conditions: 1.5% cellulase Onzuka RS, 0.5% pectinase Y-23, 0.6 mol· L~(-1) mannitol, pH 5.8, digestion time 2 h, and temperature 27℃. After isolation and purification, protoplasts were cultured in solid and liquid combined medium, which included KM8P, 0.6 mol· L~(-1) mannitol, 1.0 mg· L~(-1) 2, 4-D, 0.2 mg· L~(-1) NAA and 0.2 mg· L~(-1) KT. The division of the protoplasts first emerged in medium after cultured 4 days, and emerged only a few times.     

基于Ansys的液压钻机底盘稳态载荷分析

根据液压钻机的实际工作状态,研究履带架的受力特点,通过建立实体简易液压钻机整体结构模型,介绍一种有限元简便分析方法,对履带架结构的受力状况进行了系统分析,从而确定了履带架实际工作中的几种危险工况,并结合危险工况对履带架进行了有限元分析,得到更精确的履带架的强度分布,为履带架设计提供更准确的依据。     

气候变暖对石家庄冬小麦主要生育期的影响及对策

通过对石家庄近50年来气候资料和20多年来冬小麦主要生育期变化规律的分析,结果表明:20世纪90年代以来,石家庄冬春季升温明显,与之相适应的,冬小麦春季各生育期均有不同程度的提早,特别是1998年以后,各生育期提早更明显;通过对冬小麦各生育期与不同时段气温的相关分析可见,各生育期与春季气温呈反相关,对大多数生育期而言,它与返青到该生育期之间平均气温的相关性最好。在此基础上,提出了适应气候变化的冬小麦栽培管理对策。