H5N2与H5N1禽流感油乳灭活疫苗免疫鹅群对H5N1流行株攻毒的保护效力及母源抗体对免疫效力的干扰

应用H5N2和H5N12种类型禽流感油乳灭活疫苗分别免疫鹅群,观察比较了二者对H5N1型高致病性禽流感病毒的攻毒保护效力。结果表明,在同等剂量免疫条件下,从发病率、死亡率和泄殖腔排毒规律3项指标综合评价来看,H5N1灭活苗水禽免疫组对高致病性禽流感H5N1流行株攻毒的保护效率较H5N2灭活苗理想,且水禽禽流感母源抗体对灭活苗免疫具有一定的干扰作用。     

堆型艾美球虫3-1E基因在大肠杆菌中的可溶性表达及鉴定

将堆型艾美球虫（E．acervulina）3-1E基因克隆至pET-32a（＋）载体中。转化大肠杆菌BL21，在37℃下，用终浓度为1.0mmol／L的IPTG诱导表达，得到相对分子质量约为38500的重组蛋白。Western blot检测证实，该重组蛋白可以与特异性抗血清结合。在20℃条件下，以终浓度分别为2．0、1．0、0．5mmol／L的IPTG进行诱导．当IPTG终浓度为0．5mmol／L时，以可溶性形式存在的目的蛋白含量最高。     

灵芝组方保肝的研究

按照国家保健食品审批标准，对灵芝组方后提取有效部份，进行保肝实验，研究结果表明．以0.67、2．00、6．00g／kgBw剂量的灵芝益甘粉灌胃给予小鼠30d，并以四氯化碳造成急性肝损伤模型．灵芝益甘粉067、6．00g／kgBW剂量组的AST，6．00g／kgBW剂量组肝细胞坏死病变程度均低于模型对照组．差异有显著性（P〈0．05）．灵芝益甘粉对化学性损伤有保护功能．     

Significance of expression of extracellular matrix metalloproteinase inducer and endothelin converting enzymes in lymphoid metastasis and prognosis in non-small-cell lung carcinoma

AIM: To investigate the expression of extracellular matrix metalloproteinase inducer (EMMPRIN) and endothelin converting enzymes (ECE) in non-small-cell lung carcinoma (NSCLC), and their relationship with lymphoid metastasis and prognosis.METHODS: Expression of EMMPRIN and ECE in 77 cases of patients with NSCLC was detected immunohistochemically.The relationship of the expression of EMMPRIN and ECE with tumor size, histological type, differentiation, lymphoid metastasis, clinical stage, and prognosis were analyzed.RESULTS: The expressive rate of EMMPRIN and ECE was 66% and 45%, respectively.The expression of EMMPRIN and ECE was associated positively with lymphoid metastasis (both P<0.01, r=0.371 and 0.467, respectively), and inversely with survival time (P<0.01 and P<0.01).No relationship was found in the expression of EMMPRIN and ECE with tumor size, histological type, and differentiation (P>0.05).The expression of EMMPRIN was associated with the expression of HGF in NSCLC (P<0.05, r=0.243).CONCLUSION: The expression of EMMPRIN and ECE is associated with lymphoid metastasis and prognosis in NSCLC.Overexpression of EMMPRIN and ECE implies infavourable prognosis in NSCLC.     

Relationship between uPA and NF-κB p65 expression and its clin ical significance in breast cancer

AIM:To evaluate the relationship between u PA and NF-κB p65 expression and its clinical significance in breast cancer.METHODS:The uPA mRNA was measured by real-time fluorescent quan t itative PCR in 46 cases of breast cancer tissues and their adjacent counterparts .NF-κB p65 were measured using immunohistochemistry.RESULTS:The expression of uPA gene was elevated in 63% of cases ,and there was a strong correlation between NF-κB p65 and uPA expression (r ＝0.451，P<0.01).In addition,the expression of uPA was associated with num bers of axillary lymph node involvement and tumor size.However,no significance was found in uPA expression in the age,pathological type and distant metastasi s organ classification.CONCLUSION:The uPA gene is highly expressed in most of the brea st cancer,and there is strong correlation to NF-κB p65 expression,number of l ymph nodes involvement and tumor size.     

95%杀螟丹SP防治萧氏松茎象成虫的药效试验

试验结果表明：95％杀螟丹可溶性粉剂对萧氏松茎象成虫有良好的击倒作用。其1000倍稀释浓度对萧氏松茎象成虫的死亡率达100％，且药效在林间的持效时间可达10天。因此建议在生产上推广应用。     

苹果种质资源主要描述标准比较分析

种质资源描述标准是研究和利用种质资源的基础。介绍了国内外7个主要苹果种质资源描述标准的制定国家、发布时间、性状选择以及评价方法,并对其植物学特征和生物学性状进行归纳、整理,比较分析了各标准在描述符性质、层次结构、描述符性状选择以及编码方式等方面的差异。指出我国今后若进一步修订与完善苹果种质资源性状描述标准,可从3个方面进行借鉴:描述性状选择以必选性状为主;样本采集应严格规定取样数量、取样方法及砧木的一致性;性状鉴定以过程控制为主,兼顾结果控制,增加可操作性和可重复性。     

金针菇单核出菇与子实体颜色

利用原生质体单核化技术从8个金针菇栽培菌株中获得13种不同的黄色单核体菌株和3种白色单核体菌株;单核体出菇实验结果表明,5种黄色单核体和2种白色单核体可以形成单核子实体,其中,黄色单核子实体间在菌柄、菌盖颜色方面差异较大,而且部分单核子实体本身的菌盖和菌柄的颜色也具有明显的差异,说明控制子实体黄颜色的因子存在复等位因子。     

氮源对番茄灰霉病菌菌丝生长的影响

选用15种常见氮源,应用固体和液体培养方法,筛选适宜番茄灰霉病菌生长的氮源营养物质.结果表明:在固体培养中,病菌对氮源的利用以蛋白胨为最好,其次为酵母膏.在液体培养中,病菌对氮源的利用以酵母膏为最好,其次为(NH4)2SO4.