烟草粉螟不同烟叶存放区域消长规律

为探明烟草粉螟在烟叶仓储时的发生规律,以便实施精准防控。在施秉县不同烤房群、烟农 库房及收购点仓库,利用性诱剂对烟草粉螟进行监测。结果表明烟草粉螟在不同烟叶存放区域的始 发期、高峰期和终结期都不尽相同,同一种存区域的烟草粉螟消长规律也不相同。     

毕节市油茶产业发展现状及对策

油茶作为世界四大木本食用油料植物之一,在林业产业和山区经济发展中占有十分重要的 地位。近年来,毕节将油茶产业作为助推脱贫攻坚的重要支柱产业之一,大力发展。文章介绍了毕节 市近年推进油茶产业的一些主要做法,并指出了当前发展油茶产业存在的一些问题,针对问题,提供 了现阶段的一些对策,以期为促进全市油茶产业发展,助推脱贫攻坚提供一定的理论依据。     

Effects of sediment load on the abrasion of soil aggregate and hydraulic parameters in experimental overland flow

The breakdown of soil aggregates under rainfall and their abrasion in overland flow are important processes in water erosion due to the production of more fine and transportable particles and, the subsequent significant effect on the erosion intensity. Currently, little is known about the effects of sediment load on the soil aggregate abrasion and the relationship of this abrasion with some related hydraulic parameters. Here, the potential effects of sediment load on soil aggregate abrasion and hydraulic parameters in overland flow were investigated through a series of experiments in a 3.8-m-long hydraulic flume at the slope gradients of 8.7 and 26.8%, unit flow discharges from 2×10~(–3) to 6×10~(–3) m~2 s~(-1), and the sediment concentration from 0 to 110 kg m~(–3). All the aggregates from Ultisols developed Quaternary red clay, Central China. The results indicated that discharge had the most significant(P0.01) effect on the aggregates abrasion with the contributions of 58.76 and 60.34%, followed by sediment feed rate, with contributions of 39.66 and 34.12% at the slope gradients of 8.7 and 26.8%, respectively. The abrasion degree of aggregates was found to increase as a power function of the sediment concentration. Meanwhile, the flow depth, friction factor, and shear stress increased as a power function along with the increase of sediment concentration at different slope gradients and discharges. Reynolds number was obviously affected by sediment concentration and it decreased as sediment concentration increased. The ratio of the residual weight to the initial weight of soil aggregates(Wr/Wi) was found to increase as the linear function with an increasing flow depth(P=0.008) or Reynolds number(P=0.002) in the sediment-laden flow. The Wr/Wi values followed a power function decrease with increasing friction factor or shear stress in the sediment-laden flow, indicating that friction factor is the best hydraulic parameter for prediction of soil aggregate abrasion under different sediment load conditions. The information regarding the soil aggregate abrasion under various sediment load conditions can facilitate soil process-based erosion modeling.     

Long-term fertilization leads to specific PLFA finger-prints in Chinese Hapludults soil

Soil microbes play essential roles in the biogeochemical processes of organic carbon and nutrient cycling. Many studies have reported various short-term effects of fertilization on soil microbes. However, less is known about the effects of longterm fertilization regimes on the rhizosphere. Therefore, the objective of this study was to explore how the soil microbial communities in the rhizosphere respond to different long-term fertilization strategies. Based on a 21-year field treatment experiment in Guizhou, China, we extracted phospholipid fatty acids(PLFAs) to determine the microbial community structure in both the non-rhizosphere(NR) and rhizosphere(R). Six treatments were included: no fertilizer(CK), mineral nitrogen fertilizer(N), N with potassium(NK), phosphorus with K(PK), NPK, and NPK combined with manure(MNPK). The results showed that total PLFAs under unbalanced mineral fertilization(N, NK and PK) were decreased by 45% on average in the NR compared with CK, whereas MNPK increased fungi and G~– bacteria abundance significantly in both the NR(by 33 and 23%) and R(by 15 and 20%), respectively. In addition, all microbial groups in the R under these treatments(N, NK and PK) were significantly increased relative to those in the NR, except for the ratio of F/B and G~+/G~–, which might be due to the high nutrient availability in the R. Soil pH and SOC significantly regulated the soil microbial community and structure, explaining 51 and 20% of the variation in the NR, respectively. However, the rhizosphere microbial community structure was only significantly affected by soil pH(31%). We concluded that the soil microbial community in the NR was more strongly affected by long-term fertilization than that in the R due to the rhizosphere effect in the agricultural ecosystem. Rhizosphere nutrient conditions and buffering capacity could help microbial communities resist the change from the long-term fertilization.     

中国茶园有害生物防控40年

总结了改革开放40年来,我国茶园有害生物防控领域取得的重要研究进展,分析了现阶段我国茶园有害生物防控存在的问题,提出了相应的发展对策,为我国茶园有害生物防控研究工作提供参考。     

辽宁省果树科学研究所梨育种回顾与展望

作者对辽宁省果树科学研究所近40年来育种工作进行了系统的回顾,包括杂交育种、芽变选种、实生选种等不同育种方法及在梨果实性状遗传规律方面取得的成就等。并对未来的育种趋势进行了展望。     

大数据视角下的食用菌电商发展路径

鉴于目前食用菌电商在发展过程中面临着食用菌电商流程亟待完善、缺乏食用菌安全体系、缺乏食用菌电商人才以及缺乏知名食用菌品牌等困境,从食用菌生产销售主体角度,基于大数据视角出发,利用大数据优化食用菌电商流程,建设以大数据为支撑的食用菌安全体系,以大数据应用能力为导向培养食用菌电商人才,依托大数据建设知名食用菌品牌。     

饥饿情况下蠋蝽种内互残行为发生时期及影响

为明确饥饿情况下蠋蝽种内残杀行为的发生时期和影响因素,指导蠋蝽规模化繁育和释放,本文研究了不同发育时期和密度下的蠋蝽种内残杀行为。研究发现：蠋蝽对卵粒的种内互残行为主要发生在饥饿24 h以内,雌性蠋蝽对卵粒的取食行为显著强于雄性蠋蝽,取食比例是雄性的2.80～3.25倍、取食量是雄性的18.75～21.20倍,雌性取食下的卵粒羽化率为70.66%,显著低于雄性和对照的86.59%和87.88%。发育时期和饲养密度均显著影响蠋蝽的种内残杀行为,主要发生在饥饿后的19.63～36.00 h,并且1、2、3龄若虫没有表现出种内残杀行为,4龄以后种内残杀行为显著;低密度下蠋蝽种内互残行为不显著,但高密度会增强种内互残行为,且随密度的增加,种内互残行为显著增强。     

2019年美国玉米高产竞赛简报

介绍2019年美国玉米高产竞赛情况和竞赛结果,分析全美优胜者的分布区域、选用种子品牌及信息,进而分析玉米高产原因,从而提出美国玉米高产竞赛对我国玉米生产的启示和建议。     

Effects of calpain-2 and autophagy-related protein 5 on hepatocyte apoptosis induced by endoplasmic reticulum stress

AIMTo investigate the effects of calpain-2 and autophagy-related protein 5 (Atg5) on apoptosis of BRL-3A rat normal liver cells during endoplasmic reticulum stress (ERS) induced by dithiothreitol (DTT). METH?ODS: BRL-3A cells were treated with DTT at 2.0 mmol/L for 0, 6, 12 and 24 h to induce ERS. Real-time cell analysis (RTCA) was used to measure the effect of DTT on BRL-3A cell proliferation. Apoptosis and cell cycle distribution were analyzed by flow cytometry. The mRNA expression of calpain-2 and Atg5 was detected by real-time PCR. The protein levels of calpain-2, Atg5, Atg7, Atg12 and microtubule-associated protein 1 light chain 3 (LC3) were determined by Western blot. The interaction between calpain-2 and Atg5 was investigated by co-immunoprecipitation (Co-IP). RESULTSThe proliferation of BRL-3A cells treated with DTT was significantly inhibited. The apoptosis of BRL-3A cells was significantly increased after DTT treatment for 6, 12 and 24 h as compared with 0 h group (P<0.05). The cell cycle was arrested in G₁ phase after DTT treatment (P<0.05). After DTT treatment for 6, 12 and 24 h, the mRNA expression of calpain-2 and Atg5 in the BRL-3A cells was significantly increased as compared with 0 h group (P<0.05). The protein levels of calpain-2, Atg12 and Atg7 in the cells treated with DTT for 6, 12 and 24 h were significantly higher than those in 0 h group, and the ratio of LC3-II/LC3-I was also significantly higher than that in 0 h group, while Atg5 expression was significantly lower than that in 0 h group (P<0.05). The results of Co-IP found that the anti-calpain-2 antibody precipitated Atg5 protein from the cell lysates, and the anti-Atg5 antibody also precipitated calpain-2 from the cell lysates, which confirmed the interaction between calpain-2 and Atg5. CONCLUSION Calpain-2 may participate in ERS-induced hepatocyte apoptosis by interacting with Atg5.