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AIM: To investigate the effect of berberine (Ber) on the activation and proliferation of T lymphocytes and its mechanism of action. METHODS: Whole peripheral blood from normal subjects was stimulated with phytohemagglutinin (PHA) or phorbol ester (PDB) plus ionomycin (Ion) and the expression levels of CD69 and CD25 were evaluated with flow cytometry after the staining with appropriate fluorescent monoclonal antibody. The distribution of cell cycles was analyzed by propidium iodide staining and dead cells by 7-aminoactinomycin live staining. RESULTS: 100 μmol/L and 50 μmol/L of Ber had significant inhibition of the expression of CD69 on T cells stimulated with PDB plus Ion or PHA, while effect of 25 μmol/L Ber was not significant. And as time of action extended, the extent of inhibition decreased. For the expression of CD25, Ber at the concentrations as above all exerted significant inhibitory effect in a dose-dependent manner. Moreover, Ber could block lymphocytes cell cycle progression from G0/G1 phase to S and G2/M phase without phase specificity. Besides, live staining analysis revealed that Ber did not have significant cytotoxicity on lymphocytes. CONCLUSIONS: Ber significantly inhibits the expression of early and mid activation antigens of T cells and also blocks the progression of lymphocytes cell cycles. These results suggest that Ber exerts immunosuppression effect through inhibiting the activation and proliferation of T cells. 相似文献
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大豆疫病的种子处理技术研究 总被引:2,自引:0,他引:2
本试验用不同浓度的瑞毒锰锌、杀毒矾和克露进行大豆种子处理试验,结果表明,500ppm瑞毒锰锌对大豆疫病具有很好的治疗作用,而用杀毒矾以种子重量0.4—0.5%的剂量闷种,能显著地抑制大豆疫霉菌的侵入 相似文献
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分析植物酸性转化酶基因的保守区序列,设计一对PCR引物,以君子兰基因组DNA为模板,采用PCR方法扩增出长约500 bp的DNA片段,克隆入pGEM-TEasy载体,测序结果表明获得君子兰酸性转化酶基因家族的一个成员CMCW1,该基因片段长518 bp,不含内含子,编码172个氨基酸。其序列已在GenBank中登记(登记号为AY151269)。在GenBank中进行同源性检索的结果表明,该成员编码的氨基酸与其它植物细胞壁酸性转化酶编码的氨基酸同源性较高。 相似文献
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AIM: To explore interaction and biological behaviour changes of two kinds of cells-blastocysts and hepatocarcinoma cells in the same microenvironment. METHODS:The models of mouse blastocysts co-cultured with human hepatocarcinoma cell lines were established, then biological behaviours and mutual effects of the two kinds of cells in co-culture system were observed. RESULTS: Compared with control group, hepatocarcinoma cells with differently invasive and metastatic potential significantly enhanced the rates of blastocyst hatchment , attachment and outgrowth(P<0.05). There was no significant difference in those among hepatocarcinoma cells co-cultured groups (P>0.05). The blastocyst hatched and attached to hepatocarcinoma cells with differently invasive and metastatic potential. Then, differential trophoblasts invaded hepatocarcinoma cells. The clear-cut interfaces were gradually formed between both sides. Hepatocarcinoma cells on interface showed changes of growth direction and cell shapes and did not invade blastocysts. CONCLUSIONS: Hepatocarcinoma cells promoted blastocyst development. Blastocysts implanted and invaded hepatocarcinoma cells with differently invasive and metastatic potential in vitro, which indicate that blastocyst implantation in vitro does not relate with the kinds and differential level of interactional cells and the low selectivity maybe relate with high adaptability of early life. 相似文献
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农民旅游市场开发浅议 总被引:12,自引:1,他引:12
近几年 ,我国国内旅游发展迅速 ,城镇居民旅游市场日益成熟 ,无论出游人口还是旅游花费都明显增加 ,但农民旅游市场却没有得到应有的重视 ,旅游供给严重不足。鉴于农民旅游的重要地位 ,本文分析了当前农民旅游特征及其发展潜力 ,在此基础上尝试性地提出了开拓农民旅游市场的几点措施建议。 相似文献