全文获取类型
收费全文 | 449篇 |
免费 | 29篇 |
专业分类
林业 | 31篇 |
农学 | 22篇 |
130篇 | |
综合类 | 19篇 |
农作物 | 24篇 |
水产渔业 | 11篇 |
畜牧兽医 | 194篇 |
园艺 | 11篇 |
植物保护 | 36篇 |
出版年
2024年 | 1篇 |
2023年 | 3篇 |
2022年 | 3篇 |
2021年 | 18篇 |
2020年 | 22篇 |
2019年 | 15篇 |
2018年 | 14篇 |
2017年 | 22篇 |
2016年 | 23篇 |
2015年 | 24篇 |
2014年 | 21篇 |
2013年 | 41篇 |
2012年 | 43篇 |
2011年 | 28篇 |
2010年 | 25篇 |
2009年 | 15篇 |
2008年 | 31篇 |
2007年 | 24篇 |
2006年 | 21篇 |
2005年 | 17篇 |
2004年 | 12篇 |
2003年 | 10篇 |
2002年 | 13篇 |
2001年 | 4篇 |
2000年 | 5篇 |
1999年 | 3篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1995年 | 4篇 |
1994年 | 2篇 |
1993年 | 2篇 |
1992年 | 2篇 |
1991年 | 3篇 |
1988年 | 1篇 |
1986年 | 1篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1980年 | 1篇 |
排序方式: 共有478条查询结果,搜索用时 156 毫秒
141.
Hanna Bolibok Monika Rakoczy-Trojanowska Aneta Hromada Robert Pietrzykowski 《Euphytica》2005,146(1-2):109-116
Summary The aim of this study was to investigate the efficiency of ISSR, SSR, and SAMPL marker systems in detecting genetic polymorphism
among 30 winter rye inbred lines and to compare the results of cluster analysis performed on data from these marker systems
using different statistical methods and coefficients. Each marker system was able to discriminate among the materials analyzed
with the lowest value of average genetic similarity (GS) obtained with ISSR markers (0.2888) and the highest with SAMPLs (0.5381).
EST-derived SSRs turned out to be less efficient in detecting genetic diversity than those from genomic libraries (average
GS values 0.3814 and 0.3221, respectively). The average GS value for combined SSR data was 0.3569. The lack of correlations
between similarity and cophenetic matrices obtained with various methods systems suggests that different marker systems should
be used simultaneously for a genetic diversity study to exploit as many sources of polymorphisms as possible. 相似文献
142.
143.
144.
Monika Bugno-Poniewierska Barbara Kij Wojciech Witarski Magdalena Wojtaszek Anna Radko Angelika Podbielska Izabela Szczerbal William J. Murphy 《Reproduction in domestic animals》2020,55(9):1139-1144
The tortoiseshell coat colour is characteristic to female cats, and its occurrence in tomcats is very rare and associated with chromosome abnormalities (additional copy of X chromosome). The aim of this study was identification of the genetic basis of a case of tortoiseshell colour in a fertile Maine coon tomcat. Cytogenetic and molecular genetic studies were carried out with painting molecular probes (WCPP) specific to the X and Y sex chromosomes as well as a DNA microsatellite panel for the parentage verification of cats. Cytogenetic analysis revealed only a single set of sex chromosomes typical for male – 38,XY. The results of the microsatellite polymorphism obtained from DNA showed three alleles in locus FCA201 and four alleles in loci FCA149 and FCA441 in different tissues (blood, hair roots and testicles). Based on these results, the case was diagnosed as a true chimerism 38,XY/38,XY. To the best of our knowledge, this is the first case of a 38,XY/38,XY chimera diagnosed in cats, confirmed by genetic analysis. 相似文献
145.
Agnieszka Nowak Joanna Kochan Ewa Świętek Barbara Kij Sylwia Prochowska Wojciech Witarski Monika Bugno-Poniewierska Wojciech Niżański 《Reproduction in domestic animals》2020,55(8):992-997
The aim of this study was to evaluate the applicability of the Cryotech technique for the vitrification of domestic cat (Felis catus) oocytes, as a model for other feline species threatened with extinction. This technique, in which oocytes are stored in a minimal volume of medium, is already widely used in human assisted reproductive technology. In the first part of this study, a viability test (EtBr/FDA) was used to evaluate the toxicity of the vitrification media (solutions). After IVM, oocytes were placed in vitrification and warming solutions according to the manufacturer's procedure, with or without exposure to liquid nitrogen. The solutions and the vitrification procedure each caused a reduction in oocyte viability, with survival rates of 71.4% in oocytes exposed to the Cryotech media (without cooling in liquid nitrogen), and 62% in oocytes that were vitrified. In the second part of the experiment, parthenogenetic activation was used to evaluate the developmental potential of oocytes previously vitrified using the Cryotech method. After warming, the oocytes were activated using a combination of 0.7 µM ionomycin in TCM 199 medium (5 min) followed by 2 mM 6-DMAP in TCM 199 supplemented with 10% FBS (3 hr), then cultured and evaluated every 24 hr for parthenogenetic cleavage. In the experimental group, 23/50 (46%) cleaved embryos were obtained. Domestic cat oocytes, vitrified by the Cryotech method, are characterized by high survival rates. However, it is necessary to improve the technique to increase the developmental competence of embryos obtained from vitrified oocytes. 相似文献
146.
147.
Dolejska M Jurcickova Z Literak I Pokludova L Bures J Hera A Kohoutova L Smola J Cizek A 《Veterinary microbiology》2011,149(3-4):513-516
The aim of the study was to compare the prevalence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli bovine isolates on a conventional dairy cattle farm with high consumption of parenteral and intramammary cephalosporins (farm A) and on an organic dairy farm with no cephalosporin use (farm B). ESBL-producing E. coli were isolated from rectal swabs and milk filters by selective cultivation on MacConkey agar with cefotaxime (2mg/l). ESBL genes were identified by polymerase chain reaction (PCR) and sequencing, and the genetic diversity of the isolates was determined by XbaI pulsed field gel electrophoresis (PFGE). Conjugative transfer, incompatibility group, and restriction fragment length polymorphism (RFLP) profiles of the ESBL-carrying plasmids were studied. Higher prevalence (39%, n(rectal samples in cows)=309) of CTX-M-1-producing E. coli isolates was found on farm A compared to farm B (<1%, n(rectal samples in cows)=154; 0%, n(rectal samples in calves)=46). Using PFGE, the isolates from farm A were divided into nine pulsotypes. In all ESBL-positive isolates, the bla(CTX-M-1) gene was carried on 40 kb IncN conjugative plasmids of three related HincII restriction profiles. Horizontal gene transfer through transmission of IncN plasmids harboring bla(CTX-M-1) as well as clonal dissemination of a particular clone seems to be involved in dissemination of CTX-M-1-producing E. coli isolates in cows on the farm using cephalosporins in treating bacterial infections. The study demonstrates a possible role of cephalosporin use in the widespread occurrence of CTX-M-1-producing E. coli on the conventional dairy cattle farm compared to the organic farm. 相似文献
148.
Zołotajkin M Ciba J Kluczka J Skwira M Smoliński A 《Water, air, and soil pollution》2011,216(1-4):571-580
The research was carried out in the spruce forests of Barania Góra (Silesian Beskids, Poland) affected by pandemic dying of trees. Twenty-seven samples were collected from the O layer in two plots: 17 in a cut down forest infested with insect pests (bark beetle) and ten in a 120-year-old healthy forest. The analyses covered basic parameters (pH(H2O), pH(KCl), w(org), C(tot), N(tot), CEC) and the concentrations of aluminium in the fractions leached with 0.1?M BaCl(2) (Al(exch)), 0.5?M CuCl(2) and 0.1?M Na(4)P(2)O(7) (Al(bio)) solutions. The total aluminium concentration in the soil was assayed digesting samples with hydrofluoric acid. The effect of pH and organic matter content on the amount of exchangeable (Al(exch)) and bioavailable (Al(exch)) aluminium in the soil was analysed. It has been found that the soils in both plots are strongly acidic and contain 550 to 1,700?mg?kg(-1) of exchangeable aluminium and 1,200 to 4,800?mg?kg(-1) of bioavailable aluminium. The lack of disease symptoms in the spruce trees in plot 2 can be explained by the higher content of organic matter in the soil. Unfortunately, one might expect that the high concentration of exchangeable aluminium will also cause the trees in the area to wither. 相似文献
149.
Although the aroma compounds of meat processed as such have been studied previously, data on complete homemade dishes containing beef and pork meat were scarcely studied. Recently, 38 odor-active compounds were characterized in beef and pork vegetable gravies using GC-olfactometry. In the present investigation, the most odor-active compounds were quantitated in a freshly prepared stewed beef vegetable gravy (BVG) as well as a stewed pork vegetable gravy (PVG) by means of stable isotope dilution assays. Calculation of odor activity values (OAVs; ratio of concentration to odor threshold) revealed 3-mercapto-2-methylpentan-1-ol, (E,E)-2,4-decadienal, (E,Z)-2,6-nonadienal, (E)-2-decenal, (E)-2-undecanal, and 3-hydroxy-4,5-dimethyl-2(5H)-furanone as the most potent odorants in both gravies. However, significantly different OAVs were found for 12-methyltridecanal, which was much higher in the BVG, whereas (E,Z)-2,4-decadienal showed a clearly higher OAV in the PVG. Aroma recombination experiments performed on the basis of the actual concentrations of the odorants in both gravies revealed a good similarity of the aromas of both model mixtures containing all odorants with OAVs > 1 with those of the original gravies. 相似文献
150.