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151.
García-González DL Barie N Rapp M Aparicio R 《Journal of agricultural and food chemistry》2004,52(25):7475-7479
A novel electronic nose based on solid-phase microextraction (SPME) coupled with a surface acoustic wave (SAW) sensor array has been used to analyze different quality virgin olive oils. A mathematical model was designed with 37 samples to distinguish lampante from the other virgin olive oils categories (extra-virgin and virgin), because lampante-virgin olive oils cannot be consumed without a previous refining process. The model, successfully validated with a test set of 16 samples, was able to classify 90% of the samples correctly. Misclassifications were explained by SPME-HRGC analyses and a second sensory evaluation. 相似文献
152.
M. Belén Hinojosa José A. Carreira Richard P. Dick 《Soil biology & biochemistry》2004,36(10):1559-1568
Heavy metal contamination can inhibit soil functions but it is often difficult to determine the degree of pollution or when soil reclamation is complete. Enzyme assays offer potential as indicators of biological functioning of soils. However, antecedent water content of soil samples may affect the outcome of biological measurements. In Mediterranean regions, for much of the year ‘field moist’ surface soil can have water content similar to that of air-dry samples. The objectives of this study were to: (1) determine the sensitivity of a range of enzyme assays to detect the degree of pollution from a heavy metal mine spill; (2) evaluate rewetting field-dry soil as a pre-treatment for enzyme assays; and (3) test multivariate analysis for improving discrimination between polluted, reclaimed and non-polluted soils. The Aznalcóllar mining effluent spill provided a unique opportunity to address these objectives. This accident released toxic, heavy metal-contaminated (As, Bi, Cd, Cu, Pb, Tl, Zn…) and acid tailings into the Guadiamar watershed (SW Spain) in 1998, severely affecting the riparian zone along more than 4000 ha. Contaminated soils were collected from the highly polluted upper watershed and less polluted lower watershed along with reclaimed soil at both sites. Enzyme activities (phosphatases, arylsulfatase, β-glucosidase, urease and dehydrogenase) were assessed on both field-moist samples and soils rewetted to 80% of water-holding capacity and then incubated at 21 °C for 7 d prior to the assay. The reclaimed soils had higher activities than polluted soils but, typically, 1.5-3 times lower levels of activity than the non-polluted soil. Regardless of the moisture pre-treatment, all enzymes showed significant effects due to pollution, with urease and β-glucosidase showing the greatest discrimination between degrees of contamination. In general, rewetting field-dried soils increased activities on non-polluted and reclaimed soils which improved discrimination with polluted soils. Another method to increase the potential of soil enzyme activities to detect soil contamination could be to combine them in multivariate analysis, which provides a more holistic representation of the biochemical and microbial functionality of a soil. 相似文献
153.
Sanchez R Vázquez A Riquelme D Villén J 《Journal of agricultural and food chemistry》2003,51(21):6098-6102
A fully automated on-line reversed phase liquid chromatography-gas chromatography system is described. The system uses a prototype of the automated through oven transfer adsorption desorption interface. The system is demonstrated by presenting a new rapid method for the determination of pesticide residue in olive oil, which is injected directly with no sample pretreatment step other than filtration. Methanol:water is used as the eluent in the LC preseparation step, while the LC fraction containing the pesticide is automatically transferred to the gas chromatograph. Detection limits of pesticides varied from 0.18 to 0.44 mg/L when a flame ionization detector was used. As an example, relative standard deviation and linear calibration are presented for terbutryne. 相似文献
154.
Fernández PL Pablos F Martín MJ González AG 《Journal of agricultural and food chemistry》2002,50(7):1833-1839
155.
Enzyme-mediated solvent extraction of carotenoids from marigold flower (Tagetes erecta) 总被引:2,自引:0,他引:2
Barzana E Rubio D Santamaria RI Garcia-Correa O Garcia F Ridaura Sanz VE López-Munguía A 《Journal of agricultural and food chemistry》2002,50(16):4491-4496
Marigold flowers are the most important source of carotenoids for application in the food industry. However, the extraction gives almost 50% losses of the carotenoids depending on conditions for silaging, drying, and solvent extraction. In the past decades, macerating enzymes have been successfully applied to improve the extraction yield of valued compounds from natural products. In this work, an alternative extraction process for carotenoids is proposed, consisting of a simultaneous enzymatic treatment and solvent extraction. The proposed process employs milled fresh flowers directly as raw material, eliminating the inefficient silage and drying operations as well as the generation of hard to deal with aqueous effluents present in traditional processes. The process developed was tested at the 80 L scale, where under optimal conditions a carotenoid recovery yield of 97% was obtained. 相似文献
156.
N. Zapata E. Playán A. Martínez-Cob I. Sánchez J.M. Faci S. Lecina 《Agricultural Water Management》2007
In this paper, a contribution to the design of collective pressurised irrigation networks in solid-set sprinkler-irrigated windy areas is presented. The methodology is based on guaranteeing minimum on-farm performance, using a historical hourly wind speed database and a ballistic solid-set irrigation simulation model. The proposed method was applied to the Montesnegros Irrigation District (central Ebro basin, Spain). The district irrigates an area of 3493 ha using an on-demand schedule. The average wind speed in the area is 2.8 m s−1. An analysis of district water records showed that farmers often reduce water demand when the wind speed is high, but their irrigation decision making is limited by the capacity of the irrigation network and by the unpredictable character of local winds. Simulations were performed for 11 irrigation seasons, 2 triangular sprinkler spacings (18 m × 18 m and 18 m × 15 m), and 2 sprinkler models. The percentage of monthly suitable time for irrigation was determined for four management strategies. The first one was based on a wind speed threshold (3 m s−1), while the other three were based on three levels (standard, relaxed and restrictive) of two irrigation performance parameters: the Christiansen Uniformity Coefficient (CU) and the Wind Drift and Evaporation Losses (WDEL). The standard strategy classified the time as suitable for irrigation when CU ≥ 84% and WDEL ≤ 20%. The thresholds limits of the irrigation parameters for the relaxed strategy were CU ≥ 80% and WDEL ≤ 25%. Finally, the restrictive strategy used thresholds of CU ≥ 90% and WDEL ≤ 15%. The suitable time for the first strategy (56%) was always lower than for the standard and the relaxed strategies (with respective average values of 75 and 86%), and higher than for the restrictive strategy (30%). In order to design the collective network, the hydrant operating time was equalled to the suitable time for irrigation. The differences in the cost of the collective network plus the on-farm equipment were particularly relevant between the restrictive strategy and the other three. Differences in suitable operating time were clear between sprinkler spacings, and less evident between sprinkler models. The application of the proposed methodology may be limited by the availability of historical wind speed records and CU estimates for different combinations of sprinkler models, sprinkler spacings and wind speed. 相似文献
157.
Subsurface drip irrigation and reclaimed water quality effects on phosphorus and salinity distribution and forage production 总被引:3,自引:0,他引:3
M.P. Palacios-Díaz V. Mendoza-Grimn J.R. Fernndez-Vera F. Rodríguez-Rodríguez M.T. Tejedor-Junco J.M. Hernndez-Moreno 《Agricultural Water Management》2009,96(11):1659-1666
In the Canary Islands, water scarcity is one of the constraints for agricultural activity. Non-conventional water resources generally represent more water volume than conventional ones. The distribution of these resources frequently permits the possibility of a conjunctive use of desalinated (DW) water and reclaimed municipal wastewater (RW). Field testing with both water qualities and different irrigation systems is necessary for optimal site-specific management. The objective of this work was to evaluate soil salinity and phosphorus distribution, and alfalfa yield in a 20 month field experiment carried out in the island of Gran Canaria, using municipal RW and freshwater (FW) under subsurface drip irrigation (SDI). Phosphorus speciation was performed both in irrigation waters and in soils (Olsen's inorganic, organic, and microbial). RW had large EC values (2.4 dS m−1) with a remarkable nutrient load contribution and an average total P around 3 mg L−1, predominantly hydrolysable forms, while FW had very low salinity and negligible amounts of P. For the RW treatment a salt gradient was established, causing plant mortality between the irrigation lines. The study of P speciation allows describing P distribution and plant uptake in terms of P forms. Large values of microbial P were produced for the two irrigation waters around the emitters, especially for FW.A faster P-cycling could have contributed to the significantly larger inorganic P contents observed in FW irrigated soils, in spite no external sources were added by the irrigation water. 相似文献
158.
Gratacós-Cubarsí M Castellari M Hortós M García-Regueiro JA Lametsch R Jessen F 《Journal of agricultural and food chemistry》2008,56(19):9312-9316
Effect of tetracycline (TC) administration on the proteomic profile of pig muscle was evaluated by 2D electrophoresis and MALDI-TOF mass spectrometry. The TC content at slaughter was determined in L. dorsi samples by HPLC-DAD. Mean residual concentration of TC in the muscle of treated animals, calculated as the sum of TC and epi-TC was 126.3 microg/kg, indicating a rapid elimination of TC in this tissue. Several differential spots (n = 54, p < 0.05) were observed in protein profiles from control and treated animals. MALDI-TOF identification gave a positive match for 5 differential spots, that is, glycerol-3-phosphate dehydrogenase 1 (G3PD1), phosphoglycerate kinase 1, novelprotein (0610037L13Rik), leucine aminopeptidase 3 (LAP), and hypothetical protein isoform 2. Results show that proteomics could be a useful tool to reveal pharmacological treatments with TC, even if the possible uses of differential spots as biomarkers to detect illegal administration of TC require further studies. Different spot patterns as a consequence of TC treatments seem to be another interesting issue for the consequences on tissue metabolism and meat quality. 相似文献
159.
Abugoch LE Romero N Tapia CA Silva J Rivera M 《Journal of agricultural and food chemistry》2008,56(12):4745-4750
The amino acid composition and the physicochemical and functional properties of quinoa protein isolates were evaluated. Protein isolates were prepared from quinoa seed by alkaline solubilization (at pH 9, called Q9, and at pH 11, called Q11) followed by isoelectric precipitation and spray drying. Q9 and Q11 had high levels of essential amino acids, with high levels of lysine. Both isolates showed similar patterns in native/SDS-PAGE and SEM. The pH effect on fluorescence measurements showed decreasing fluorescence intensity and a shift in the maximum of emission of both isolates. Q9 showed an endotherm with a denaturation temperature of 98.1 degrees C and a denaturation enthalpy of 12.7 J/g, while Q11 showed no endotherm. The protein solubility of Q11 was lower than that of Q9 at pH above 5.0 but similar at the pH range 3.0-4.0. The water holding capacity (WHC) was similar in both isolates and was not affected by pH. The water imbibing capacity (WIC) was double for Q11 (3.5 mL of water/g isolate). Analysis of DSC, fluorescence, and solubility data suggests that there is apparently denaturation due to pH. Some differences were found that could be attributed to the extreme pH treatments in protein isolates and the nature of quinoa proteins. Q9 and Q11 can be used as a valuable source of nutrition for infants and children. Q9 may be used as an ingredient in nutritive beverages, and Q11 may be used as an ingredient in sauces, sausages, and soups. 相似文献
160.
Detrital carbon accumulation accounts for most of an ecosystem's capacity to store organic carbon because the carbon contained as plant detritus exceeds that stored in living plants by about threefold. A comparative analysis of the mass and turnover of detrital carbon in ecosystems demonstrates that these properties are strongly related to the turnover rate of the dominant primary producers and are poorly related to ecosystem primary production. These results contribute to an understanding of the factors that control carbon storage in ecosystems and the role of carbon storage in the global carbon budget. 相似文献