QTL mapping of powdery mildew susceptibility in hop (Humulus lupulus L.)

Hop powdery mildew [Podosphaera macularis (Wallr.) U. Braun & S. Takam.] is best controlled via the production of resistant varieties. Recent evidence supports selection against plant susceptibility genes to fungal pathogens as a more durable resistance mechanism than selection for resistance genes. The objective of this study was to identify molecular-based QTLs, their genetic effects and epistasis among QTLs associated with susceptibility to powdery mildew. Parents and offspring from the cross, ‘Perle’ × ‘USDA 19058M’, were clonally replicated and inoculated in a greenhouse using a CRD experimental design in Corvallis, OR. DNA was extracted, purified and analyzed via three different marker systems. Analysis of the resulting markers was based upon the “two-way pseudo-testcross” procedure. QTL mapping using multiple interval mapping and Bayesian interval mapping analyses were performed using WinQTL Cartographer 2.5_003. Comparison amongst mapping analyses identified three persistent QTLs on three linkage groups without significant epistatic effect upon expression. The persistent QTL on linkage group C7 had both additive and dominant effects controlling phenotype expression. The presence or absence of the two AFLP markers bordering the QTL on C7 defined susceptibility in offspring. This is the first report in hop identifying molecular markers linked to QTLs associated with disease susceptibility.     

Exceeding the threshold value for Trioza apicalis Förster 1848 in carrot fields did not cause damage as revealed during monitoring in Germany from 2017–2020

Journal of Plant Diseases and Protection - The carrot psyllid Trioza apicalis Förster 1848 is a carrot pest in Europe that can cause serious damages in case of massive occurrence. Damages up...     

Agroforestry policy issues and research directions in the US and less developed countries: insights and challenges from recent experience

Efforts to improve the performance of agroforestry systems, and to expand the land area and number of people able to benefit from this integrative approach to agriculture and natural resource management, are constrained throughout the world by non-supportive land use policies. A growing sense of urgency that policy change is needed to enable agroforestry to flourish has contributed during the past two years to an unprecedented level of agroforestry policy assessment and planning activity.In the US, agroforestry has emerged from academia, where it has incubated since the mid-1980s, into the professional resource management arena. A multi-organizational agroforestry evaluation process has driven national policy and program formation to the forefront of the agenda of the agroforestry community, as it seeks to influence the 1995 Farm Bill. Internationally, the Consultative Group on International Agricultural Research and collaborators fostered a sequence of policy issue identification activities as a basis for setting strategic research priorities for forestry and agroforestry.Following a brief review of forces driving agroforestry development in industrialized and less developed countries, the paper highlights recent policy assessment initiatives in each sphere. Observations on the issues driving and the priorities emerging from these processes are offered, to lend perspective to the critical challenges facing the agroforestry policy research community. An explanation for pervasive constraints and inconsistencies in policy effectiveness is then explored, from which a promising approach to research intervention is forwarded.It is argued that social scientists might influence agroforestry policy most favorably at this critical juncture, as perceptions of inter-dependence increase among different stakeholders in the policy system, by employing interventionist, actor-oriented perspectives and participatory methods to facilitate policy innovation and evaluation. The approach is consistent with participatory technology design processes that earlier helped to establish agroforestry as a prototype for sustainable development.     

Analysis of a backcross population from a multi-copy transgenic Brassica napus line

Brassica napus is one of the crops at the forefront of biotechnological development. The procedures used to produce transgenic varieties are all prone to generating plants with multiple transgene copies. There has been considereable interest in the behaviour of such multi-copy lines because gene dosage rarely appears to correlate simply with gene expression. Here we report the analysis of a population of 107 progeny from a B. napus transformant containing multiple copies of a GUS marker gene construct. A total of 12 GUS sequence copies were identified including one that was non-functional. The expression of GUS increased with increasing copy number but this increase only made up a small proportion of the total variation between lines. There was no evidence of interaction between the various GUS copies and they appeared to segregate independently. The variation between progeny lines indicated that the number of gene copies was not a good guide to the expression of the gene product and hence that the expression of the gene in progeny from a multiple-copy parent could not be predicted. The importance of these findings in relation to plant breeding and the risk assessment process is discussed. This revised version was published online in August 2006 with corrections to the Cover Date.     

Identification and characterization of T-DNA inserts by T-DNA fingerprinting

A T-DNA fingerprinting method is presented based on amplified fragmentlength polymorphism with an anchored polymerase chain reaction step. Thismethod allows discrimination between different T-DNA inserts in stablytransformed plants. The technique was evaluated by analyzing 51 transgenicArabidopsis lines that had been characterized in detail by genomicblotting. Comparison of the obtained fingerprints with the availableintegration information demonstrated that fingerprints were correlated tothe predicted patterns, except for the inverted repeat junctions and forthose inserts with large deletions at the left or right border. Ourexperiments show that by using T-DNA fingerprinting multi-copy transgeniclines can be eliminated efficiently so that the technique can be used toenrich a population of transgenic plants for putative single-copytransformants.     

Propofol–medetomidine–ketamine total intravenous anaesthesia in thiafentanil–medetomidine-immobilized impala (Aepyceros melampus)

Objective

To characterize a propofol–medetomidine-ketamine total intravenous anaesthetic in impala (Aepyceros melampus).

Diagnosis of bromethalin toxicosis in the dog

Dogs given a single oral dose of bromethalin at 6.25 mg/kg developed a toxic syndrome characterized by hyperexcitability, tremors, seizures, depression, and death within 15-63 hours after bromethalin administration. Gross lesions included mild cerebral edema (2/5) and mild pulmonary congestion (2/5). Histologic lesions included diffuse white matter spongiosis (5/5), mild microgliosis (3/5), optic nerve vacuolization (3/5), mild thickening of Bowman's capsule (2/5), and occasional splenic megakaryocytes (2/5). Ultramicroscopic examination of midbrain stem revealed occasional swollen axons, intramyelinic vacuolization, and myelin splitting at the intraperiod line. Bromethalin was detected in kidney, liver, fat, and brain tissues, using gas chromatography with electron capture detection. Photodegradation of extracted bromethalin may limit accurate quantification of tissue residues.     

Acute aflatoxicosis in feeder pigs, resulting from improper storage of corn

Aflatoxicosis was diagnosed in 600 feeder pigs, of which 400 died, 150 were destroyed, and 50 were marketed. The pigs were exposed to 2,500 to 3,500 micrograms of aflatoxins/kg of feed. Drought-stressed damaged corn infected with Aspergillus flavus was stored under ambient conditions in a glass-lined silo, and this storage environment provided conditions that favored rapid fungal growth and mycotoxin production.