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61.
Two vaccines, based on formalin-killed whole cells of toxigenic Pasteurella multocida type D and Bordetella bronchiseptica combined with a partially toxoided cell extract of P multocida, were prepared with Freund's incomplete adjuvant (vaccine 1) or by alum precipitation (vaccine 2). Each was tested for safety and efficacy in reducing the severity of nasal turbinate atrophy and improving the growth rate of pigs in three Western Australian commercial piggeries with endemic atrophic rhinitis. In safety experiments with vaccine 1, no adverse clinical effects were observed in vaccinated sows or their progeny. Piglets receiving vaccine 2 showed no injection site abnormalities, pyrexia or turbinate atrophy. In field trials, vaccine 1 significantly reduced the prevalence of moderate to severe nasal turbinate atrophy (Done score 3 to 5) when used in two piggeries (A and B). Progeny from vaccinated sows in piggery B also grew significantly faster than controls. When vaccine 2 was used in piggery A at a later date and in another piggery (C), growth rate was not improved in either piggery and the prevalence of moderate to severe turbinate atrophy was reduced only in piggery C. 相似文献
62.
With the commercial release in Australia in 2004 of a vaccine against feline immunodeficiency virus (FIV; Fel‐O‐Vax FIV®), the landscape for FIV diagnostics shifted substantially. Point‐of‐care (PoC) antibody detection kits, which had been the mainstay for diagnosing FIV infection since the early 1990s, were no longer considered accurate to use in FIV‐vaccinated cats, because of the production of vaccine‐induced antibodies that were considered indistinguishable from those produced in natural FIV infections. Consequently, attention shifted to alternative diagnostic methods such as nucleic acid detection. However, over the past 5 years we have published a series of studies emphasising that FIV PoC test kits vary in their methodology, resulting in differing accuracy in FIV‐vaccinated cats. Importantly, we demonstrated that two commercially available FIV antibody test kits (Witness? and Anigen Rapid?) were able to accurately distinguish between FIV‐vaccinated and FIV‐infected cats, concluding that testing with either kit offers an alternative to PCR testing. This review summarises pertinent findings from our work published in a variety of peer‐reviewed research journals to inform veterinarians (particularly veterinarians in Australia, New Zealand and Japan, where the FIV vaccine is currently commercially available) about how the approach to the diagnosis of FIV infection has shifted. Included in this review is our work investigating the performance of three commercially available FIV PoC test kits in FIV‐vaccinated cats and our recommendations for the diagnosis of FIV infection; the effect of primary FIV vaccination (three FIV vaccines, 4 weeks apart) on PoC test kit performance; our recommendations regarding annual testing of FIV‐vaccinated cats to detect ‘vaccine breakthroughs’; and the potential off‐label use of saliva for the diagnosis of FIV infection using some FIV PoC test kits. We also investigated the accuracy of the same three brands of test kits for feline leukaemia virus (FeLV) diagnosis, using both blood and saliva as diagnostic specimens. Based on these results, we discuss our recommendations for confirmatory testing when veterinarians are presented with a positive FeLV PoC test kit result. Finally, we conclude with our results from the largest and most recent FIV and FeLV seroprevalence study conducted in Australia to date. 相似文献
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Johnson JC Bryson WL Barringer S Hunsaker BD 《Veterinary therapeutics : research in applied veterinary medicine》2008,9(1):53-62
This study was designed to compare morbidity and mortality due to bovine respiratory disease (BRD) in 1,400 ranch-fresh, northern US calves that received either no metaphylaxis (MTX) (Group 1), MTX prompted by pen morbidity (Group 2), MTX prompted by a decline in feed intake (Group 3), or on-arrival MTX (Group 4). Ceftiofur crystalline free acid (6.6 mg/kg) was the MTX antimicrobial used. Decreased feed intake did not meet the study threshold, so Groups 1 and 3 were combined into a single control group. The percentage of calves not treated for BRD was 14% to 15% higher in Groups 4 and 2 (72.7% and 73.4%, respectively) compared with the controls (58.2%; P less than or equal to .02). Mortality was numerically higher in Group 2 (3.4%) compared with Groups 4 and 1 (0.9% and 1.6%, respectively). There were no differences in average daily gain or feed consumption. In northern, ranch-fresh calves, MTX as a health management practice reduces the incidence of BRD and has better utility when performed on-arrival versus relying on morbidity as a signal for timing antimicrobial administration. 相似文献
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JM Castillo JC Dockweiler SH Cheong M Diel de Amorim 《Reproduction in domestic animals》2018,53(1):274-277
A 13‐year‐old Romanov sheep presented for evaluation of vaginal discharge, depression and anorexia. Blood work demonstrated mature neutrophilia and marked hyperlactatemia. Transrectal and transabdominal ultrasound revealed echogenic fluid (pus) within the uterus. Purulent uterine fluid culture yield Escherichia coli. A diagnosis of pyometra was made. After medical treatment without clinical improvement, an exploratory laparotomy was performed, and a 360‐degree uterine left horn torsion was identified with the pyometra and multiple corpora lutea on both ovaries. Ovariohysterectomy was performed and the ewe recovered uneventfully. 相似文献
69.
ABSTRACT The fitness and the dynamics of demethylation inhibitor fungicide (DMI) sensitivity in isolates of Monilinia fructicola sensitive (no growth at 0.3 mg/liter propiconazole) and resistant (>/=50% relative growth at 0.3 mg/liter propiconazole) to propiconazole were investigated. Overall, there was no considerable compromise in the fitness of resistant isolates compared to sensitive isolates of M. fructicola at the time of collection. Resistant and sensitive isolates differed in their sensitivity to propiconazole (P < 0.001) and incubation period (P = 0.044), but not in latent period, growth rate, spore production, and spore germination frequency (P > 0.05). Consecutive transferring on potato dextrose agar had an impact on conidia production, conidial germination, and growth rate (P < 0.0001). Consecutive transferring also had an impact on propiconazole sensitivity in resistant isolates. In the resistant isolates, sensitivity to propiconazole increased (R(2) = 0.960, P = 0.0034) within the first eight transfers. Similarly, sensitivity to propiconazole increased by 273% over the course of 34 months in cold storage in propiconazole-resistant isolates. Our results show that propiconazole resistance is unstable in vitro and that standard subculturing and cold storage procedures impact propiconazole sensitivity of resistant isolates. The instability of propiconazole resistance in M. fructicola may have important implications for disease management in that a reversion to propiconazole sensitivity could potentially occur in the absence of DMI fungicide pressure in the field. 相似文献
70.
The objective of this study was to determine the species composition and geographic distribution of ixodid ticks infesting domestic dogs in the eastern region of the Eastern Cape Province. Seventy-two communal cattle dip-tanks within this region were randomly selected as survey localities and their geographic coordinates recorded. In addition to ticks that were collected from five cattle and five goats at each of the dip-tanks, ticks were also collected from five dogs whenever possible. No dogs were available at 19 dip-tanks and no ticks were collected from dogs at 13 dip-tanks, while ticks were collected from 132 of 200 dogs at 40 dip-tanks. Eight ixodid tick species were collected from these dogs, and Haemaphysalis leachi followed by Rhipicephalus appendiculatus and Rhipicephalus simus were present on dogs at the largest number of dip-tanks. Seven dogs were simultaneously infested with three tick species and one with four species. The geographic distributions of Amblyomma hebraeum, H. leachi, R. appendiculatus and R. simus recovered from the dogs lay within the ranges previously reported for these ticks. 相似文献