昆明鼠卵母细胞的去核及其去核率的观察

本文研究昆明鼠卵母细胞的去核方法，并通过对去核卵母细胞的固定染色，显示其中期Ⅱ染色体，由此观察统计其确定的去核率。     

水稻不育系粒黑粉病普遍率与严重度间的关系

 稻粒黑粉病(病原菌Neovossia horrida (TaK.)Padw. et A. Kahn)是杂交稻繁殖制种田不育系上的主要病害,流行频率高,危害损失大。普遍率和严重度的关系(I-S关系)是植病流行学观测技术上的一个重要问题。     

山东绿篱树种的种质资源及其应用

在调查山东绿篱应用现状的基础上，从了解山东绿篱树种的种质资源状况着手，针对其种类、应用方式、生长特性和适应性挖掘利用方面进行分析。认为目前在绿篱种质资源应用方面存在一些问题，针对这些问题提出了一些建议。     

履带板用高锰钢强韧化研究

本文根据履带板的失效特征和机理,提出了高锰钢的铬、钼再合金化,稀土、钛变质处理和重结晶热处理工艺综合强韧化方案.经实验证实,综合机械性能显著改善.     

‘Plantibodies’: a flexible approach to design resistance against pathogens

Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (V_L) and heavy chain (V_H) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location.     

嫁接对茄子果实中蛋白质、VC、可溶性糖含量及分布的影响

以托鲁巴姆为砧木、西安绿茄为接穗进行嫁接,研究嫁接对茄子果实中蛋白质、VC、可溶性糖含量及分布的影响。结果表明,嫁接后除可溶性糖含量略低于对照外,蛋白质、VC及果实中的含水量均高于对照。嫁接苗果实中主要营养成分在果实各部位的分布规律基本不变,内果皮中各种养分的含量均高于外果皮,由果实顶部至蒂部各种养分的变化规律不尽相同。随着果实的膨大,嫁接苗果实中主要营养成分的变化规律与对照基本一致。瞪眼期果实中的蛋白质含量较高,进入商品成熟期后,果实中的养分含量基本不变,趋于稳定。     

樟子松在西北干旱沙区的光合日变化特征

在甘肃省民勤治沙站 ,用 Li- 62 0 0便携式光合仪分别测定人工栽植的 4龄、9龄和 2 0龄樟子松的净光合速率日进程 ,其结果表明 :在干旱沙区 ,9龄以上樟子松的光合速率在午前随光强和气温而上升 ,午后下降。其 4龄、9龄和 2 0龄樟子松的最大净光合速率分别为 8.7μmol· m- 2 · s- 1、62 .3μmol· m- 2 · s- 1、1 9.7μmol· m- 2 · s- 1。     

Effects of Latent Infection of Stock Plants and Abundance of Thrips on the Occurrence of <Emphasis Type="Italic">Tomato spotted wilt virus </Emphasis>in Chrysanthemum Fields

DAS-ELISA proved to be reliable enough to detect a latent infection by Tomato spotted wilt virus (TSWV) in asymptomatic stock plants of chrysanthemum. A high density of Frankliniella occidentalis, the predominant vector, in the presence of latently infected stock plants resulted in a high incidence of disease in the chrysanthemum production field. The incidence of disease was low when the vector thrips were not abundant in spite of the presence of latently infected stock plants. These results suggest that an infestation of the vector thrips causes severe secondary spread of TSWV originating from latently infected stock plants in chrysanthemum production fields. Received 27 July 2001/ Accepted in revised form 27 November 2001