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21.
Wang Dechun Prato Tony Qiu Zeyuan Kitchen Newell R. Sudduth Kenneth A. 《Precision Agriculture》2003,4(1):35-52
Variable Rate Technology (VRT) has the potential to increase crop yields and improve water quality relative to Uniform Rate Technology (URT). The effects on profitability and water quality of adopting VRT for nitrogen (N) and lime were evaluated for corn production on four claypan soil fields in north central Missouri under average to better than average weather conditions. Variable N and lime rates were based on measured topsoil depth and soil pH, respectively. VRT rates were compared to two different uniform N applications (URT-Nl based on the topsoil depth within these claypan soil fields, and URT-N2 based on a typical N rate for corn production in this area). Expected corn yield was predicted based on topsoil depth, soil pH, N rate, and lime rate. Water quality benefits of VRT relative to URT were evaluated based on potential leachable N. Sensitivity analyses were performed using simulated topsoil data for topsoil depth and soil pH. Results showed that VRT was more profitable than URT in the four sample fields under URT-N1, and in two of the four fields under URT-N2. Greater variation in topsoil depth and soil pH resulted in higher profitability and greater water quality benefits with VRT. Results support adoption of VRT for N and lime application for other claypan soil fields with characteristics similar to those in the fields used in this study. 相似文献
22.
Bakal C Linding R Llense F Heffern E Martin-Blanco E Pawson T Perrimon N 《Science (New York, N.Y.)》2008,322(5900):453-456
Cellular signaling networks have evolved to enable swift and accurate responses, even in the face of genetic or environmental perturbation. Thus, genetic screens may not identify all the genes that regulate different biological processes. Moreover, although classical screening approaches have succeeded in providing parts lists of the essential components of signaling networks, they typically do not provide much insight into the hierarchical and functional relations that exist among these components. We describe a high-throughput screen in which we used RNA interference to systematically inhibit two genes simultaneously in 17,724 combinations to identify regulators of Drosophila JUN NH(2)-terminal kinase (JNK). Using both genetic and phosphoproteomics data, we then implemented an integrative network algorithm to construct a JNK phosphorylation network, which provides structural and mechanistic insights into the systems architecture of JNK signaling. 相似文献
23.
Frudakis T 《Science (New York, N.Y.)》2008,319(5866):1039-40; author reply 1039-40
24.
Femtosecond laser irradiation is used to excite adsorbed CO molecules on a Cu110 surface; the ensuing motion of individual molecules across the surface is characterized on a site-to-site basis by in situ scanning tunneling microscopy. Adsorbate motion both along and perpendicular to the rows of the Cu110 surface occurs readily, in marked contrast to the behavior seen for equilibrium diffusion processes. The experimental findings for the probability and direction of the molecular motion can be understood as a manifestation of strong coupling between the adsorbates' lateral degrees of freedom and the substrate electronic excitation produced by the femtosecond laser radiation. 相似文献
25.
26.
The brain keenly depends on glucose for energy, and mammalians have redundant systems to control glucose production. An increase in circulating glucose inhibits glucose production in the liver, but this negative feedback is impaired in type 2 diabetes. Here we report that a primary increase in hypothalamic glucose levels lowers blood glucose through inhibition of glucose production in rats. The effect of glucose requires its conversion to lactate followed by stimulation of pyruvate metabolism, which leads to activation of adenosine triphosphate (ATP)-sensitive potassium channels. Thus, interventions designed to enhance the hypothalamic sensing of glucose may improve glucose homeostasis in diabetes. 相似文献
27.
Engelman JA Zejnullahu K Mitsudomi T Song Y Hyland C Park JO Lindeman N Gale CM Zhao X Christensen J Kosaka T Holmes AJ Rogers AM Cappuzzo F Mok T Lee C Johnson BE Cantley LC Jänne PA 《Science (New York, N.Y.)》2007,316(5827):1039-1043
The epidermal growth factor receptor (EGFR) kinase inhibitors gefitinib and erlotinib are effective treatments for lung cancers with EGFR activating mutations, but these tumors invariably develop drug resistance. Here, we describe a gefitinib-sensitive lung cancer cell line that developed resistance to gefitinib as a result of focal amplification of the MET proto-oncogene. inhibition of MET signaling in these cells restored their sensitivity to gefitinib. MET amplification was detected in 4 of 18 (22%) lung cancer specimens that had developed resistance to gefitinib or erlotinib. We find that amplification of MET causes gefitinib resistance by driving ERBB3 (HER3)-dependent activation of PI3K, a pathway thought to be specific to EGFR/ERBB family receptors. Thus, we propose that MET amplification may promote drug resistance in other ERBB-driven cancers as well. 相似文献
28.
Justyna Zofia Paplinska David A. Taggart Tony Corrigan Mark D.B. Eldridge Jeremy J. Austin 《Biological conservation》2011,(1):290-297
Use of DNA from museum samples is a powerful tool to directly establish historical ESU boundaries in areas where populations of a species have been extirpated. The brush-tailed rock-wallaby (Petrogale penicillata) has suffered extensive reductions in range and population size since European settlement in Australia. Populations of this species have been grouped into three putative evolutionarily significant units (ESUs) which were likely once contiguous along the south-east coast of Australia. However, there is currently a gap of ∼320 km between extant populations of the southern and central ESUs. Conservation plans for the brush-tailed rock-wallaby include re-introductions of animals to locations within their former range. In order to preserve the historic geographic integrity of the genetic lineages within the species, it is necessary to map the boundary between the southern ESU and central ESU to allow more informed management decisions about which lineages should be used to re-stock specific geographic locations. We have extracted DNA from samples from museum specimens that come from locations between the southernmost extant colony of the central ESU and the remaining wild colony of the southern ESU. We sequenced a 177 bp interval of the left domain of the mitochondrial DNA control region and used phylogenetic analysis to group obtained sequences with previously published sequences belonging to the three ESUs. We have extended the range of the central ESU southwards and the range of the southern ESU northwards such that the gap between the ESUs is now approximately 160 km. We recommend that, where suitable historical museum collections exist, this technique be incorporated into re-introduction plans for other threatened species. 相似文献
29.
Amy M. Treonis Roger Cook Lorna Dawson Susan J. Grayston Tony Mizen 《Biology and Fertility of Soils》2007,43(5):541-548
We studied the effects of the root endoparasitic nematode Heterodera trifolii on rhizodeposition and the root architecture of white clover (Trifolium repens). Rhizosphere solutions were collected from the root systems of plants growing with and without H. trifolii (200 juveniles per inoculated plant) in sand-based microlysimeters. The organic carbon (C) content of these solutions was
analyzed, and they were applied to plant-free soils to investigate microbial responses. Although plant biomass was unaffected
by nematodes, the architecture of the root systems was significantly altered, with a decrease in overall root length and an
increase in the density of lateral branches from the primary root. The presence of nematodes reduced the concentration of
organic compounds in the rhizosphere solutions but only on the final sampling date (75 days). Analysis of microbial signature
phospholipid fatty acids revealed no change in the structure of the microbial communities in soils to which rhizosphere solutions
were applied. However, these microorganisms did respond with changes in substrate utilization patterns (community-level physiological
profiles). Microbes in soils that received rhizosphere solutions from the nematode-infected clover showed lower utilization
of most substrates but higher utilization of oligosugars. These responses appear to be related to changes in roots and rhizodeposition
associated with nematode infection of clover roots. The results of this study suggest that root herbivory can negatively impact
carbon-limited soil microbial communities via changes in root architecture that moderate rhizodeposition. 相似文献
30.
Anthocyanin glycosides from berry fruit are absorbed and excreted unmetabolized by both humans and rats 总被引:10,自引:0,他引:10
McGhie TK Ainge GD Barnett LE Cooney JM Jensen DJ 《Journal of agricultural and food chemistry》2003,51(16):4539-4548
Anthocyanins, the red/blue pigments found in plants, are polyphenolic compounds consumed by humans and are part of a normal diet. Recent studies have shown that anthocyanins have substantial bioactivity including antioxidant activity and therefore may have beneficial effects on human health. Anthocyanins are a group of over 500 compounds of diverse structures containing different core phenolic aglycons and conjugated with sugars in a variety of glycosylation patterns. In this study, we have investigated the bioabsorption of 15 anthocyanins with structures containing different aglycons and conjugated sugars extracted from blueberry, boysenberry, black raspberry, and blackcurrant in both humans and rats. Intact and unmetabolized anthocyanins were detected in urine of rats and humans following dosing for all molecular structures investigated, thus demonstrating that anthocyanins with diverse molecular structure and from different dietary sources are bioavailable at diet relevant dosage rates. In addition, the relative concentrations of anthocyanins detected in urine following dosing varied, indicating that differences in bioavailability are due to variations in chemical structure. Our results suggest that the nature of the sugar conjugate and the phenolic aglycon are both important determinants of anthocyanin absorption and excretion in rats and humans. 相似文献