用重组核衣壳蛋白ELISA检测PRRSV抗体的研究

ＰＲＲＳＶ重组Ｎ蛋白ＥＬＩＳＡ检测人工感染猪血清７５份,与ＩＤＥＸＸ公司ＥＬＩＳＡ试剂盒及ＩＦＡ的符合率分别为９７．３％和１００％。检测疫苗免疫猪血清,阳性率１００％。在免疫后６ｄ就能检出抗体,比ＩＦＡ敏感。检测８５份我国田间猪血清,阳性率１８．８％。     

不同激素剂量对幼鼠超数排卵及超排卵质量的影响

应用国产激素制剂对３～８周龄昆明种小白鼠进行了超数排卵处理,重点观察了不同激素剂量对幼龄小鼠的超排效果及超排卵质量的影响。实验结果表明,以ＰＭＳＧ与ｈＣＧ各１０ＩＵ剂量超排３～８周龄小鼠,５周龄小鼠的超排效果（平均获卵８３．８±１０．２个）极显著（Ｐ＜０．０１）好于４～８周龄小鼠的超排效果（平均获卵３９．２～４９．８个）,而３周龄小鼠超排卵受精后体外培养的卵裂率（９７．１％）、桑椹胚率（８６．８％）和囊胚率（６５．１％）与４～８周龄超排卵的（分别为８５．５％～１００．０％、９４．９％～１００．０％和６３．２％～８７．７％）相近;以ＰＭＳＧ与ｈＣＧ各５ＩＵ剂量超排３～５周龄小鼠,３周龄小鼠的超排效果（平均获卵５９．０±６．５个）显著（Ｐ＜０．０５）好于４周龄小鼠的超排效果（平均获卵３１．４±４．９个）,并极显著（Ｐ＜０．０１）好于５周龄小鼠的超排效果（平均获卵２１．４±３．３个）;以ＰＭＳＧ与ｈＣＧ各１５ＩＵ剂量超排３～５周龄小鼠,３周龄小鼠的超排效果（平均获卵４８．６±７．３个）显著（Ｐ＜０．０５）好于５周龄小鼠的超排效果（平均获卵２５．２±５．８个）,也好于４周龄小鼠的超排效果（平均获卵４０．２±５．１个     

不同贮藏条件鸡蛋的脂类过氧化作用

本文以ＴＢＡ光度法分析了不同贮藏条件下鸡蛋脂类的过氧化作用,并分析了温度、氧对过氧化作用的影响。结果表明,在贮藏条件下,鸡蛋蛋黄中伴有明显的脂类过氧化过程。脂类过氧化物（ＬＰＯ）的水平随贮藏时间的延长而不断增加（Ｐ＜０．０１）,但蛋清中的ＬＰＯ在贮藏过程中没有明显的变化。低温和缺氧可明显抑制鸡蛋脂类的过氧化作用（Ｐ＜０．０１）。     

Identification and validation of a yield-enhancing QTL cluster in rice (Oryza sativa L.)

Improvement of rice grain yield (YD) is an important goal in rice breeding. YD is determined by its related traits such as spikelet fertility (SF), 1,000-grain weight (TGW), and the number of spikelets per panicle (SPP). We previously mapped quantitative trait loci (QTLs) for SPP and TGW using the recombinant inbred lines (RILs) derived from the crosses between Minghui 63 and Teqing. In this study, four QTLs for SF and four QTLs for YD were detected in the RILs. Comparison of the locations of QTLs for these three yield-related traits identified one QTL cluster in the interval between RM3400 and RM3646 on chromosome 3. The QTL cluster contained three QTLs, SPP3a, SF3 and TGW3a, but no YD QTL was located there. To validate the QTL cluster, a BC₄F₂ population was obtained, in which SPP3a, SF3 and TGW3a were simultaneously mapped to the same region. SPP3a, SF3 and TGW3a explained 36.3, 29.5 and 59.0 % of phenotype variance with additive effect of 16.4 spikelets, 6 % SF and 1.8 g grain weight, respectively. In the BC₄F₂ population, though the region has opposite effects on TGW and SPP/SF, a YD QTL YD3 identified in this cluster region can increase 4.6 g grains per plant, which suggests this QTL cluster is a yield-enhancing QTL cluster and can be targeted to improve rice yield by marker aided selection.     

酒泉市动物产地检疫现状及对策

随着社会经济迅速发展,酒泉市动物及动物产品流通日益频繁,动物产地检疫极为重要。本文主要论述了酒泉市动物产地检疫的作用、意义、现状、存在的问题及解决对策和措施。     

青皮种子主要储藏条件的研究Ⅰ.种子含水量与测控

水分是影响青皮(Vatica astrotricha Hance)种子活力最关键的内在因素,其中种胚含水量是决定因素。用含水量27—29％椰糠控制种胚含水量在50—52％,也就是控制种子含水量在30—36％,维持种子一定的呼吸强度(32℃下测定为50—60 O_2μl/g·h),种子活力保持最高,能延长寿命。找出了储藏中种子安全含水量的最佳点,此点处于安全含水量下限的内缘,可利用种子本身果翅与种子或大小种子之间含水量的内在关系来测定。这种测定方法准确、快速又简便。可考虑作为确定“顽拗型”种子安全含水量下限的新捷径。     

Studies on the pathogenicity ofBabesia bovis in water buffaloes after cryopreservation and resuscitation

Tropical Animal Health and Production - Packed erythrocytes infected withBabesia bovis were mixed with an equal volume of 16% dimethyl sulphoxide (DMS0) in Alsever’s solution and dispensed...     

Equine chronic diarrhea: Traditional Chinese veterinary medicine review

Chronic diarrhea is a common problem in equine practice. Herbal medicine has been used for the treatment of gastrointestinal disease in horses for at least one thousand years. In present-day equine practice in China, the most common approach to treat chronic diarrhea is herbal medicine, or a combination of herbal medicine and acupuncture. Three types of chronic diarrhea in horses will be described in detail in term of symptoms, diagnosis and herbal treatments in this paper. Nineteen herbal formulas for treatment of different types of diarrhea will be introduced. Clinical results of each herbal formula will be evaluated. Acupuncture treatment of this problem will also be included.     

Multiple amino acid substitutions are involved in the adaptation of H9N2 avian influenza virus to mice

To explore adaptation of avian influenza virus to mice we previously performed serial lung-to-lung passages of the influenza A/Chicken/Jiangsu/7/2002 (H9N2) strain, resulting in the isolation of a variant influenza strain lethal for mice. We now report that virulence correlates with improved growth characteristics on mammalian cells and extended tissue tropism in vivo. Sequencing of the complete genomes of the wild-type and mouse-adapted viruses revealed 25 amino acid substitutions. Some were found to reiterate known substitutions in human and swine H9N2 influenza isolates. Functions affected include nuclear localization signals and sites of protein and RNA interaction, while others are known determinants of pathogenicity and host specificity such as the viral polymerase PB2 E627K substitution. These observations suggest that enhanced growth characteristics and modified cell tropism may contribute to increased virulence in mice. We conclude that multiple amino acid substitutions are likely to be involved in the adaptation of H9N2 avian influenza virus to mice.     

貂,貉,犬病毒性肠炎快速诊断试剂的研究

用金黄葡萄球菌Ａ蛋白（ＳＰＡ）协同凝集方法（ＣｏＡ）对貂、貉、犬病毒性肠炎快速诊断的试剂进行了制备，确定了制备。艺及该制剂的标准操作程序。本制剂特异性强，不与健康动物的组织及其他几种病毒病（犬瘟热、犬传染性肝炎、狂犬病等）发生交叉凝集反应，灵敏度高，通过该制剂ＣｏＡ法与ＨＡ－ＨＩ法比较结果比现行的貂、貉、犬病毒性肠炎ＨＡ－ＨＩ诊断方法检出率高出３０％，同时具有微量、操作简便、易掌握、快速等特点，可在３～５ｍｉｎ获得诊断结果。