Development of colchicine‐induced tetraploid St. Augustinegrass (Stenotaphrum secundatum) lines

St. Augustinegrass is well suited for lawns and commercial landscapes. While many genotypes are cross‐fertile, all cultivars are propagated vegetatively in sod production. To ensure varietal purity, development of sterile triploid hybrids by crossing tetraploid and diploid genotypes has been successfully used in other warm‐season turfgrasses. Applying this model in St. Augustinegrass would be beneficial to sod producers and turf managers who require purity for certification and uniformity for performance, respectively. This study was conducted to develop colchicine‐induced tetraploid lines of St. Augustinegrass. Seeds of cultivar ‘Raleigh’ were treated with four colchicine concentrations at four exposure times. A non‐treated control was included among the treatments. Seedlings that germinated were screened for genome size changes using flow cytometry. Line DSA 13005 and two progeny lines derived through selfing, DSA 16001 and DSA 16016, were corroborated as tetraploids (2n = 4x = 36) through chromosome counts. These lines will be used in future breeding efforts to attempt development of sterile triploid cultivars of St. Augustinegrass.     

Developments in the nutrition of Menidia estor Jordan 1880

The Mexican silverside, Menidia estor, is a species with great regional importance and with very high prices in local markets. Unfortunately, due to high fisheries pressure, environmental degradation and pollution, the species has become endangered. Recently, there has been much progress in the biotechnology of this species, aimed at its culture, and the present paper describes the advances in feeding and nutrition of those important fish. M. estor is a stomachless, zooplantophagous fish, which also occasionally feeds on small fish and crustaceans in the adult stage. Studies on the digestive enzymatic activities show high proteolytic capacity and a late or different model of digestive maturation from that described for marine stomach fish. Nutritional studies on M. estor have shown that juveniles have dietary requirements of about 400 g kg^?1 protein and 80 mg kg^?1 vitamin C. The upper level of dietary carbohydrate for good growth and survival for juveniles is about 150 g kg^?1. Based on the high docosahexaenoic acid (DHA) content in flesh when fed on diets low in DHA, it is believed that the species has the capacity to biosynthetize >20‐carbon fatty acids from 18‐carbon fatty acids. The high levels of DHA in flesh makes this fish a very significant potential component of human nutrition. Using these findings the first practical diets for commercial culture of the species have been developed.     

First attempts towards the restoration of gorgonian populations on the Mediterranean continental shelf

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Influence of pond fertilization and feeding rate on growth performance, economic returns and water quality in a small-scale cage-cum-pond integrated system for production of Nile tilapia (Oreochromis niloticus L.)

The effects of pond fertilization and feeding rate on growth, economic returns and water quality were investigated to develop a low‐cost cage‐cum‐pond integrated system for production of Oreochromis niloticus (L.). Hand‐sexed male fingerlings averaging 19±0.39 and 32±0.69 g were stocked in cages and open ponds at 150 fish cage^?1 and 2 fish m^?2 respectively. Fish were cultured for 114 days in five triplicate treatments. Cages were installed into ponds and caged fish were fed a 24% protein diet at 3% (T1) and 6% (T2) body weight day^?1 (BWD) without pond fertilization, and 6% BWD with pond fertilization (T3). The open water in the fourth treatment (T4) was not stocked but contained caged fish, which were fed 6% BWD for the first 57 days followed by 3% BWD for the remaining period. Ponds in the control (T5) had no cages and were neither fertilized nor open‐pond fish fed. Feeding rate and pond fertilization significantly (P<0.05) affected fish growth, profitability and water quality among treatments. Fish growth, feed utilization, fish yield, water quality and profits were significantly (P<0.05) better in T3 than the other treatments. It was concluded that fish production and economic returns were optimized at 6% BWD in fertilized ponds.     

Terrestrial-marine continuum of sedimentary natural organic matter in a mid-latitude estuarine system

Journal of Soils and Sediments - Humic acids (HA) have several environmental roles, but are particularly important in aquatic environments, being recognized as redox active natural organic matter...     

Co-inoculation of selected nodule endophytic rhizobacterial strains with Rhizobium tropici promotes plant growth and controls damping off in common bean

Production of common bean(Phaseolus vulgaris)is limited by the occurrence of damping off(rhizoctoniosis),which is caused by the fungus Rhizoctonia solani.However,the co-inoculation of plant growth-promoting rhizobacteria(PGPR)involved in biological control along with diatomic nitrogen(N2)-fixing rhizobia can enhance N nutrition and increase production.In this context,finding microorganisms with synergistic effects that perform these two roles is of fundamental importance to ensure adequate yield levels.The aim of this study was to evaluate the effects of co-inoculation of nodule endophytic strains of the genera Bacillus,Paenibacillus,Burkholderia,and Pseudomonas with Rhizobium tropici CIAT 899,an N2-fixing rhizobial strain,on the biocontrol of damping off and growth promotion in common bean plants.Greenhouse experiments were conducted under axenic conditions using the common bean cultivar Pérola.The first experiment evaluated the potential of the 14 rhizobacterial strains,which were inoculated alone or in combination with CIAT 899,for the control of R.solani.The second experiment evaluated the ability of these 14 rhizobacterial strains to promote plant growth with three manners of N supply:co-inoculation with CIAT 899 at low mineral N supply(5.25 mg N mL^-1),low mineral N supply(5.25 mg N mL^-1),and high mineral N supply(52.5 mg N mL^-1).The use of rhizobacteria combined with rhizobia contributed in a synergistic manner to the promotion of growth and the control of damping off in the common bean.Co-inoculation of the strains UFLA 02-281/03-18(Pseudomonas sp.),UFLA 02-286(Bacillus sp.),and UFLA 04-227(Burkholderia fungorum)together with CIAT 899 effectively controlled damping off.For the common bean,mineral N supply can be replaced by the co-inoculation of CIAT 899 with plant growth-promoting strains UFLA 02-281/02-286/02-290/02-293.Nodule endophytes UFLA02-281/02-286 are promising for co-inoculation with CIAT 899 in the common bean,promoting synergy with rhizobial inoculation and protection against disease.     

Does the application of silvicultural management models drive the growth and stem quality of sweet chestnut coppices towards sustainability?

New Forests - The coppice is a very flexible cultural system producing several calibers adapting to the demands of the market. Small-caliber roundwood from sweet chestnut (Castanea sativa Mill.)...     

Some effects of light intensity and photoperiod on the sea bass larvae (Dicentrarchus labrax (L.)) reared at the Centre Oceanologique de Bretagne

Four experiments were performed to determine the effects of light intensity on the growth and survival of sea bass larvae; two experiments dealt with the photoperiod and two with the combined effects of light intensity and the photoperiod. Recently hatched larvae from the same spawning were used within each experiment.The light intensity experiments show that there is better growth but poorer survival at higher light intensities. The photoperiod experiments show a better growth at 18 h photoperiod and a better survival at 12 h exposure to light. The study of the combined effects of light intensity and photoperiod confirmed the hypothesis that strong light intensities are lethal to newly hatched larvae (with no pigmentation). If the light intensity is lowered during the first week, the best rearing conditions were found to be continuous lighting relative to survival rate, and 14–16 h photoperiod relative to growth.     

Effect of dietary carbohydrate-to-lipid ratios on growth, lipid deposition and metabolic hepatic enzymes in juvenile Senegalese sole (Solea senegalensis, Kaup)

A study was undertaken to determine the effect of various dietary carbohydrate‐to‐lipid ratios on growth performance, whole‐body composition and tissue lipid content in Senegalese sole (Solea senegalensis) juveniles. Data on the dietary regulation of key hepatic enzymes of the lipogenic and glycolytic pathways (glucose‐6‐phosphate dehydrogenase, G6PD; malic enzyme, ME; fatty acid synthetase, FAS; pyruvate kinase, PK and glucokinase, GK) were also generated. Four isonitrogenous (crude protein: 52% dry matter (DM)) diets were formulated to contain one of two lipid levels (11% and 21% DM). Within each dietary lipid level, the nature of the carbohydrate fraction (raw or extruded peas) was varied. Triplicate groups of 54 sole (initial body weight: 23.6±1.2 g) were grown in recirculated seawater over 67 days. Fish were fed using automated feeders. At the end of the study, whole‐body, liver, viscera and muscle samples were withdrawn for analyses. During the experimental period, the mean fish weight about doubled in all treatments. No significant differences were found in growth performance (ranging from 1.1% to 1.4% body weight day^?1) among dietary treatments. High‐fat diets increased whole‐body fat content. Similarly, daily fat gain ranged from 0.54 to 0.78 g kg^?1 day^?1 and highest values were found in fish fed high‐lipid diets. Dietary treatments also affected tissue lipid content (liver, viscera and muscle), with highest values in fish fed high‐fat diets. The nature of dietary carbohydrates had little influence on performance criteria, but affected tissue lipid deposition. The activities of G6PD, ME and FAS were depressed by elevated levels of dietary lipid, confirming the inhibitory effect of dietary fats on lipid biosynthesis. At both dietary lipid levels, ME and FAS activities were little affected by dietary carbohydrate. Activities of PK and GK were not affected by the starch level of the diets. In Senegalese sole juveniles, the lipogenic pathway is more susceptible to modulation by dietary means (particularly through lipid intake) than the glycolytic pathway.     

Growth comparison of Asian Nile and red tilapia strains in controlled and uncontrolled farm conditions

Growth of several genetically improved Nile tilapia (GIFT or Genetically Improved Farmed Tilapia, FaST or Freshwater Aquaculture Center Selected Tilapia, SEAFDEC-selected) and domesticated red tilapia (BFS or Binangonan Freshwater Station, NIFI-red or National Inland Fisheries Institute red, HL or Hacienda Luisita) stocks were compared in controlled (tank) and uncontrolled farm conditions (lake-based cages) with unselected NIFI or Chitralada Nile tilapia as control. Specific growth rate differed significantly (P = 0.009) in tank-reared Nile tilapia stocks where GIFT grew best at 1.358%/day followed by FaST (1.307%/day), control stock NIFI (1.257%/day) and SEAFDEC-selected (1.202%/day). Genetic effect explained 84.4% of the variance in growth of Nile tilapia in tanks. Although Nile tilapia growth in cages followed the same trend where GIFT grew best at 1.570%/day, no significant stock differences (P = 0.479) were noted. Meanwhile, red tilapia reared in either tanks or cages showed no significant stock differences in terms of growth. However, survival of the red tilapia stocks in cages differed significantly with HL having the highest percentage survival at 93.3%. The different growth responses of the Nile tilapia stocks especially under controlled (tank) farm conditions were largely due to genetic factors (stock differences).Under uncontrolled farm conditions, environmental factors were generally observed to have also affected the survival and to some extent, the growth of Asian Nile and red tilapia stocks.