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51.
Berg CA 《Science (New York, N.Y.)》1973,181(4095):128-138
52.
The mechanisms by which hydrophobic molecules, such as long-chain fatty acids, enter cells are poorly understood. In Gram-negative bacteria, the lipopolysaccharide layer in the outer membrane is an efficient barrier for fatty acids and aromatic hydrocarbons destined for biodegradation. We report crystal structures of the long-chain fatty acid transporter FadL from Escherichia coli at 2.6 and 2.8 angstrom resolution. FadL forms a 14-stranded beta barrel that is occluded by a central hatch domain. The structures suggest that hydrophobic compounds bind to multiple sites in FadL and use a transport mechanism that involves spontaneous conformational changes in the hatch. 相似文献
53.
Evidence of estrogen receptors in normal human osteoblast-like cells 总被引:80,自引:0,他引:80
E F Eriksen D S Colvard N J Berg M L Graham K G Mann T C Spelsberg B L Riggs 《Science (New York, N.Y.)》1988,241(4861):84-86
In seven strains of cultured normal human osteoblast-like cells, a mean of 1615 molecules of tritium-labeled 17 beta-estradiol per cell nucleus could be bound to specific nuclear sites. The nuclear binding of the labeled steroid was temperature-dependent, steroid-specific, saturable, and cell type-specific. These are characteristics of biologically active estrogen receptors. Pretreatment with 10 nanomolar estradiol in vitro increased the specific nuclear binding of progesterone in four of six cell strains, indicating an induction of functional progesterone receptors. RNA blot analysis demonstrated the presence of messenger RNA for the human estrogen receptor. The data suggest that estrogen acts directly on human bone cells through a classical estrogen receptor-mediated mechanism. 相似文献
54.
The existing observational data for the binary pulsar PSR 1913 + 16 are sufficient to give a rather well-defined model for the system. On the basis of evolutionary considerations, the pulsar must be a neutron star near the upper mass limit of 1.2 solar masses (M.). The orbital inclination is probably high, i>/= 700, and the mass of the unseen companion probably lies close to the upper limit of the range 0.25 M. to 1.0 M.. The secondary cannot be a main sequence star and is probably a degenerate helium dwarf. At the 5.6-kiloparsec distance indicated by the dispersion measure, the magnetic dipole model gives an age of approximately 4 x 104 years, a rate of change of the pulsar period of P approximately 2 nanoseconds per day, and a surface magnetic field strength approximately (1/3) that of the Crab pulsar. The pulsar is fainter than an apparent magnitude V approximately + 26.5 and is at least approximately 80 times fainter than the Crab pulsar in the x-ray band. The companion star should be fainter than V approximately + 30, and a radio supernova remnant may be detectable near the position of the pulsar at a flux level of =10 janskys. Important tests of this model will be provided by more accurate measurement of P and by a careful search for a faint supernova remnant. 相似文献
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57.
Recent studies have implied that (Mg, Fe)SiO(3)-perovskite, a likely dominant mineral phase in the lower mantle, may have a high melting temperature. The implications of these findings for the dynamics of the lower mantle were investigated with the use of numerical convection models. The results showed that low homologous temperatures (0.3 to 0.5) would prevail in the modeled lower mantle, regardless of the effective Rayleigh number and internal heating rates. High-temperature ductile creep is possible under relatively cold conditions. In models with low rates of internal heating, local maxima of viscosity developed in the mid-lower mantle that were similar to those obtained from inversion of geoid, topography, and plate velocities. 相似文献
58.
Berg WE 《Science (New York, N.Y.)》1946,104(2711):575-576
59.
60.
A methylaspartate cycle in haloarchaea 总被引:1,自引:0,他引:1
Access to novel ecological niches often requires adaptation of metabolic pathways to cope with new environments. For conversion to cellular building blocks, many substrates enter central carbon metabolism via acetyl-coenzyme A (acetyl-CoA). Until now, only two such pathways have been identified: the glyoxylate cycle and the ethylmalonyl-CoA pathway. Prokaryotes in the haloarchaea use a third pathway by which acetyl-CoA is oxidized to glyoxylate via the key intermediate methylaspartate. Glyoxylate condensation with another acetyl-CoA molecule yields malate, the final assimilation product. This cycle combines reactions that originally belonged to different metabolic processes in different groups of prokaryotes, which suggests lateral gene transfer and evolutionary tinkering of acetate assimilation. Moreover, it requires elevated intracellular glutamate concentrations, as well as coupling carbon assimilation with nitrogen metabolism. 相似文献