小鼠胎儿成纤维细胞饲养层制备的研究

研究主要探讨在制备小鼠胎儿成纤雏饲养层中，不同处理条件对小鼠胎儿成纤雏细胞分裂与存活的影响。结果表明：小鼠胎儿成纤雏细胞用适当处理浓度的丝裂霉素-C或适当强度的γ-射线处理一定的时间(10μg／mL 1-4h,20μg／mL 1—2．5h或21Gy和28Gy各处理1h)，能有效地抑制其分裂，且不影响其活力。     

Magnetic Resonance Imaging Study of Adipose Tissues in the Head of a Common Dolphin (Delphinus delphis): Structure Identification and Influence of a Freezing–Thawing Cycle

Magnetic resonance imaging (MRI) was used to scan the head of a common dolphin (Delphinus delphis) in order to visualize the different adipose tissues involved in echolocation functioning and to precisely delineate their anatomical topology. MRI scans were performed on the head taken from a freshly stranded carcass and repeated after a 2‐week freezing time followed by thawing. The main fatty organs of the head, that is the melon, the mandibula bulba, the bursae cantantes, and their different connections with surrounding tissues were identified and labelled. The nasal sacs, other organs of echolocation, were also identified and labelled thanks to different MRI acquisitions. The shape, the location, the type of MRI signal of each organ and of their different connections were successfully analysed on all images, and then, the images of the head fresh or after thawing were compared. No impacts of the freezing/thawing cycle on the fatty tissues of the head were identified. Different parts were distinguished in the melon on the basis of the MRI signal emitted, corresponding most likely to the internal and external melon already identified by other analytical approaches, and linked to differences in lipid composition. MRI is shown here to be a useful tool to study the functional anatomy of the organs responsible for echolocation in odontocetes, with a particularly high level of precision.     

Bilateral perirenal hemorrhage in two Stone's sheep (Ovis dalli stonei): a possible manifestation of malignant catarrhal fever

Malignant catarrhal fever (MCF)-like disease was diagnosed at postmortem in 2 Stone's sheep (Ovis dalli stonei). On gross examination, the predominant abnormality in both sheep was severe perirenal hemorrhage and multiple renal infarcts. Microscopically, there was severe, multisystemic lymphocytic arteritis. Both sheep were positive for Ovine herpesvirus 2 (OvHV-2) on polymerase chain reaction, and partial sequencing of the viral DNA polymerase genes from the 2 sheep revealed >99% homology, with 96% similarity to the reference GenBank OvHV-2 viral sequence. Based on the histological lesions, polymerase chain reaction results, and viral DNA polymerase gene sequencing, a diagnosis of OvHV-2-mediated MCF was made. Massive perirenal hemorrhage has not been described previously as a manifestation of MCF.     

江西省宜春市某肉牛场布鲁菌病的流行病学调查

布鲁菌病是一种以母畜流产、暂时或永久性不育、产奶量下降为主要特征的一种人畜共患传染病,严重危害畜牧业发展和人类健康。为了掌握江西省宜春市某肉牛场布鲁菌病感染情况,以便做好布鲁菌病的预防、控制和净化工作,采用虎红平板凝集试验和试管凝集试验,对该规模化肉牛养殖场的300余头肉牛进行随机抽检。结果显示,随机抽检的60头肉牛样品中,布鲁菌病感染率为0,表明该牛场的布鲁菌病防控措施有效。     

青海互助北山林场羊肚菌营养成分初探

以青海互助北山林场羊肚菌子实体和菌丝体为试材,采用GB/T营养成分测定方法和原子吸收分光光度法,对其所含粗蛋白质、粗脂肪、粗纤维、灰分和矿质元素的含量进行分析。结果表明:羊肚菌子实体和菌丝体中均富含蛋白质、粗纤维、多种常量和微量元素,其中子实体中含有粗蛋白质24.51%、粗脂肪1.36%、粗纤维6.26%,说明羊肚菌是一种集营养、保健和药用功能于一体的优质食用菌资源。     

农药的化学信息学分析

从大英农作物生产研究会e-农药手册(The e-Pesticide Manual V5.0)中提取了1 478个各类农药,利用化学信息学方法对比了农药与临床药物在物理化学性质、分子骨架等方面的差异,分析了二者在化学空间中的分布特点。结果表明:农药具有相对较低的相对分子质量和较高的脂溶性,其F、Cl、P等元素含量明显高于临床药物;在分子骨架和化学空间分布上二者也有着显著差异。因此,在将医药研发的技术应用到农药研发时,应根据农药的特点适当调整设计或计算参数。     

Quantitative Change of Carbohydrate Content of Two Varieties of Jerusalem Artichoke Tubers (Helianthus tuberosus L.) During Cold Storage Conditions (4°C)

The effects of 2,4-D with or without Azospirillum hra-iiknse (Sp7) on nodulation of wheat (cv HD2428) have been investigated under hydropooical conditions. In the nutrient medium, varying concentrations of N and 2,4-D were used for growing wheat seedlings, N free Hoaglatid solution along with 2,4-D (0.5 ppm) was found to be the best concentration for noduiation and growth of the seedings, The nilrogenase activity (acetylene reduction y was relatively higher in plants treated with 2,4-D artd Azospirillum ihan with Azospirillum alone. This seemed to be due to the formation of nodule like stiucture serving as mche for azospirilla to protect it from oxygen. These results confirmed tlie nitrogenase activity of Azospirillun brasiknse in association with the 2,4-D induced nodules of wheat. Plants inoculated with Azospirillum either alone or with 2,4-D were transplanted info pols. The photosynthctic rate, chlorophyll content. NRA, grain yield and N content were found to be higher in inoculated plants. The translocation of current photo-synthates (¹⁴C sucrose studies) towards roots was higher in these treatments. Thtss the growth of Azospirilfum and nodule formatioo depend on availability of current photosynthatcs of hosl plants.     

Use of the skin test in the evaluation of true levels of cellular immunity and levels after experimental administration of glucan and levamisole in feed-lot bulls]

Applicability of a skin test induced by dinitrofluorobenzene (DNFB) to quantification of the actual level of cellular immunity (CI) in vivo and its level after an experimental immunomodulation intervention were evaluated in two breeds (40 animals in each) of fattening bulls (10-11 months old). At the selected methodical procedure of intensity determination of the delayed type of hypersensitivity (DTH), its average value reached 4.5 +/- 1.5 mm in 80 animals, while in 77.5% of bulls its level ranged from 3.6 to 9.6 mm, in 18.7% from 2.0 to 3.5 mm and in 3.8% remained less than 2.0 mm. Evident expression of the reaction points to the possibility of application of the used methodical procedure of the skin test using DNFB to quantify the level of CI response in vivo in cattle. Percentual representation of animals according to the intensity of skin reaction (Tab. I) and concentration of total serum immunoglobulins (Cs-Ig) and serum IgG (Tab. II) indicates the different cellular and humoral state of animals in investigated breeds. This is also confirmed by the recorded average values of mentioned parameters which were significantly lower (P less than 0.01; or 0.05) in animals of the first breed (4.0 +/- 1.3 mm; 28.3 +/- 4.4 U ZST, 18.4 +/- 3.5 g.l-1) than in breed 2 (4.9 +/- 1.6 mm; 32.5 +/- 3.8 U ZST; 20.3 +/- 3.5 g.l-1). The animals of each breed were divided into four experimental groups with the approximately equal actual levels of DTH (Tab. III).(ABSTRACT TRUNCATED AT 250 WORDS)     

Ovarian inhibition of pulsatile luteinizing hormone secretion in prepuberal holstein heifers

Fifteen prepuberal Holstein heifers were utilized to examine pulsatile luteinizing hormone (LH) secretion before and after ovariectomy. Heifers were ovariectornized at 3, 6 or 9 months of age (n=5/group) and scheduled for blood sampling at 1 week before, 1 week after and 4 weeks following ovariectomy. During each 8 hr sampling period (0600–1400 hr), blood samples (10 ml) were collected via indwelling jugular canulae at 10 min intervals. Prior to ovariectomy, mean plasma LH concentration and both number and amplitude of LH pulses per 8 hr sampling period were similar (P>.05) among age groups, and the absence of a pulsatile LH secretion profile was accompanied by a low mean LH concentration. Within 1 week after ovariectomy, both number of LH pulses and mean LH concentrations increased (P<.O1) in all age groups. Between 1 and 4 weeks after ovariectomy, both amplitude of LH pulses and mean LH concentrations increased (P<.O1) when the data from the three age groups were combined. We conclude that ovarian inhibition of pulsatile LH secretion is established by 3 months of age and is maintained through 9 months of age. In addition, the initial elevation mean plasma LH concentration is due to greater pulse frequency, while the subsequent rise in mean LH concentration reflects increased amplitude of LH pulses.