The live Brucella melitensis Rev 1 strain is considered the best vaccine available for the prophylaxis of brucellosis in small ruminants. The classically recommended exclusive vaccination of young replacement animals has failed to control brucellosis in some developed countries and is frequently inapplicable in the developing world. Accordingly, whole-flock vaccination is the only feasible alternative to control B. melitensis infection in small ruminants under the extensive management conditions characteristic of these countries. This review describes the practical problems encountered and the experience acquired over the past decade (particularly in Spain) using the Rev 1 based control strategy. The vaccination of pregnant animals with full standard doses of Rev 1 administered subcutaneously is followed by abortion in most vaccinated animals. Reducing the dose of vaccine has been suggested as a method of avoiding this problem and, accordingly, a reduced-dose vaccination strategy has been widely used and has been reported as a safe and effective method of controlling small ruminant brucellosis. However, we reviewed field and experimental results supporting the fact that as a result of the induction of abortion in pregnant animals and the low degree of immunity conferred, reduced doses of Rev 1 should not be recommended as an alternative to the full standard doses.
When tested in a mouse model, differences in residual virulence and immunogenicity have been demonstrated between the different Rev 1 vaccines produced world-wide. These differences could account for the discrepancies in safety results obtained in mass vaccination trials in different countries. The induction of abortions when vaccinating pregnant animals means that there is no entirely safe strategy for Rev 1 vaccination. Conjunctival vaccination is safer than subcutaneous vaccination but is not safe enough to be applied regardless of the pregnancy status of the animals, and should be used only under restricted conditions. For sheep, conjunctival administration of standard doses of Rev 1 during the late lambing season or during lactation is recommended as a whole-flock vaccination strategy. 相似文献
The uptake of L-leucine and L-alanine by trout cardiomyocytes was analysed to identify the carriers involved in their incorporation
into cells. The sodium independent systems L and asc, as well as the sodium dependent system ASC, were found to be present.
The Vmax of these carriers was 33.0, 40.1 and 19.4 pmol amino acid mg−1 prot min−1 for L, asc and ASC, respectively, and the Km values obtained for these transport systems were 296, 269 and 349 μM amino acid. The L system had as its main substrates
the large apolar branched-chain amino acids and showed stereospecificity. The asc and ASC systems carried the short-chain
neutral amino acids and were not inhibited by Me-AIB. The ASC transporter also showed adaptive regulation. The effect of IGF-I
and insulin on L-alanine uptake, as well as the incorporation of L-leucine into protein, were also studied. Low IGF-I concentrations
(below 2.5 ng ml−1) stimulated the uptake of L-alanine and protein synthesis but this effect reverted at high IGF-I concentrations. Insulin
depressed protein synthesis and did not affect L-alanine uptake.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
Low temperatures, and the voluntary fasting that they induce, have been implicated in outbreaks of winter syndrome in farmed
gilthead sea bream (Sparus aurata). Two experiments were performed with the objective of studying if a decrease in water temperature is sufficient to induce
this disease or some of its associated signs. In the first experiment, water temperature was either acutely or gradually reduced
to 8 °C. In the second experiment, fish were exposed to either 8 °C or 12 °C, or fasted at 14 °C.
Although no mortalities due to winter syndrome were registered during any of the experiments, some of the signs described
in affected sea bream were observed in 8 °C-exposed fish. Among the most relevant were the paleness and friability of the
liver and the occurrence of fatty degeneration in the hepatocytes.
During the experiments, the general state of health of fish was monitored by measuring hematic parameters and the plasma concentration
of proteins, glucose and ions. Low temperatures (8 and 12 °C) and fasting at 14 °C resulted in significant decreases in the
levels of the different plasma protein fractions. This drop was more important in all the 8 °C-exposed fish, due mainly to
a higher descent of albumin, α1-globulins and fibrinogen. Moreover, 8 °C-exposed fish showed a fall in total white blood cells and a rise of plasma glycemia,
as well as a significant drop of plasma potassium and calcium levels and a transient increase of plasma magnesium concentration.
In 8 °C-exposed fish, the rate of water temperature descent did not modify the profiles of change of any of the studied parameters.
These results indicate that at 8 °C gilthead sea bream are unable to maintain the levels of plasma protein fractions and ions
present in control animals.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
This study compared the growth performance, plasma testosterone and cortisol levels around castration at 10 months of age, and plasma insulin‐like growth factor (IGF)‐I concentration and flight speed, in intact bulls and steers from 10 to 21 months of age in a feral Spanish breed. Fourteen bulls (366.5 ± 48.5 kg live weight) were assigned at random to one of two treatments: surgically castrated (steers) or intact (bulls), and submitted to an identical fattening period. Steers reared until heavy live weights (21 months of age) grew slowly and had lower plasma IGF‐I concentrations than intact bulls. These differences were mainly highlighted the month after surgery (11 months of age) and the last part of the fattening period (from 19 to 21 months of age). After surgical castration (11 and 12 months of age), steers showed a tendency to display greater flight speed values than intact bulls but baseline plasma cortisol concentration did not differ between groups at this time. At the end, steers and bulls reached nearly similar temperament, as flight speed did not differ between them. The results confirm the role of IGF‐I as a key anabolic hormone in male beef cattle and thus it may reflect growth differences due to altered sex steroids production. 相似文献
The efficacy of Brucella Melitensis Rev 1 vaccine (Rev 1) for the prophylaxis of Brucella ovis ram epididymitis was evaluated. Twenty-nine 3-month-old rams were vaccinated with 2 X 10(9) Rev 1 and 14 were revaccinated with 5 X 10(8) at 14 months of age. Six rams remained unvaccinated as a control group. All rams were challenged with 5 X 10(8) B. ovis at 21 months of age. Before being slaughtered 8 weeks later, only one vaccinated ram developed epididymitis while four of the six control rams developed testicular alterations. Genital and selected extragenital organs and lymph nodes were removed at slaughter and inoculated on selective media. B. ovis was isolated from 26.6% of the vaccinated rams, 21.4% of the revaccinated rams and 100% of control rams. Portions of epididymis, testes and vesicular glands were also used for pathological studies. More severe lesions were observed in control rams than in vaccinated ones. In conclusion, these results show that vaccination of young lambs, followed or not by revaccination, is a suitable method for the prophylaxis of B. ovis infection of rams. 相似文献