A dominant gene for garnet brown seed coats at the <Emphasis Type="Italic">Rk</Emphasis> locus in ‘Dorado’ common bean and mapping <Emphasis Type="Italic">Rk</Emphasis> to linkage group 1

The color of the seed coats of ‘Dorado’ (Phaseolus vulgaris L.) is garnet brown (dark red kidney bean color) and differs from most other dry bean varieties in the Honduran red bean market class. A genetic investigation of the color of ‘Dorado’ (same as DOR364) and G19833 (Liborino market class) seed coats was conducted. Crosses with genetic tester stocks demonstrated that the gene for garnet brown (GB) in ‘Dorado’ was not allelic with the R gene for dominant red (oxblood) seed coat. An allelism test between the ‘Dorado’ gene for GB seed coat and the rk ^drv gene for recessive expression of GB demonstrated allelism. We propose the gene symbol for Rk ^r for the ‘Dorado’ GB seed coat color gene. Rk ^r expresses partial dominance over Rk, where Rk ^r/Rk expresses a paler and highly variable intermediate red color. The interactions of Rk ^r, rk ^drv, and c ^u are discussed. Segregation analysis in the mapping population made up of DOR364 (same as ‘Dorado’) × G19833 recombinant inbred lines showed that the Rk ^r gene mapped to linkage group 1. The new allele at Rk was located at a distance of 17 cM from the RFLP marker Bng130 with a LOD > 3.0.     

Long-term management impacts on soil C, N and physical fertility: Part II: Bad Lauchstadt static and extreme FYM experiments

Manure is a source of plant nutrients and can make a valuable contribution to soil organic matter (SOM). Two experimental sites were studied on a Halpic Phaeozem soil near Bad Lauchstadt in Germany. The first experiment, called the static experiment, commenced in 1902. The impact of fresh farmyard manure (FYM) (0, 20 and 30 t ha⁻¹ 2 year⁻¹) combined with P, K and N fertiliser application on total organic C (C_T), labile C (C_L), non-labile C (C_NL), total N (N_T), mean weight diameter (MWD) and unsaturated hydraulic conductivity (K_unsat) was investigated. The second experiment commenced in 1984 and investigated the effect of extreme rates of fresh FYM applications (0, 50, 100 and 200 t ha⁻¹ year⁻¹) and cropping, or a continuous tilled fallow on the same soil properties. At both sites a nearby grassland site served as a reference. On the static experiment, FYM application increased all C fractions, particularly C_L, where application of 30 t ha⁻¹ 2 year⁻¹ increased C_L by 70% compared with no FYM application. Fertiliser additions to the static experiment had a positive influence on C fractions while N_T increased from both FYM and fertiliser application. MWD increased as a result of FYM application, but did not reach that of the grassland site. Both fertiliser and FYM application increased K_unsat (10 mm tension) on the static experiment. In the second experiment application of 200 t ha⁻¹ year⁻¹ of FYM increased concentrations of C_L by 173% and of C_NL by 80%, compared with no FYM application to make them equivalent to, or greater than the grassland site. A continuously tilled fallow resulted in significant decreases in all C fractions, N_T and MWD compared with the cropped site, while K_unsat (10 mm tension) was increased on the 0 and 50 t ha⁻¹ year⁻¹ treatments as a result of a recent tillage. There was no difference in K_unsat between the cropped and the continuous tilled fallow at FYM applications of 100 and 200 t ha⁻¹ year⁻¹. There were similar significant positive correlations of all C fractions and N_T with MWD on both experimental sites but the relationships were much stronger on the extreme FYM experiment. Weaker relationships of C fractions and N_T with K_unsat (10 mm tension) occurred for the static experimental site but these were not significant for the extreme FYM experimental site. The strongest relationship between C fractions and K_unsat was with C_L. This research has shown that applications of FYM can increase SOM and improve soil physical fertility. However, the potential risk of very high rates of FYM on the environment need to be taken into consideration, especially since the application of organic materials to soils is likely to increase in the future.     

A molecular clutch disables flagella in the Bacillus subtilis biofilm

Biofilms are multicellular aggregates of sessile bacteria encased by an extracellular matrix and are important medically as a source of drug-resistant microbes. In Bacillus subtilis, we found that an operon required for biofilm matrix biosynthesis also encoded an inhibitor of motility, EpsE. EpsE arrested flagellar rotation in a manner similar to that of a clutch, by disengaging motor force-generating elements in cells embedded in the biofilm matrix. The clutch is a simple, rapid, and potentially reversible form of motility control.     

Conversion of grassland to coniferous woodland has limited effects on soil nitrogen cycle processes

In the last century, conversion of native North American grasslands to Juniperus virginiana forests or woodlands has dramatically altered ecosystem structure and significantly increased ecosystem carbon (C) stocks. We compared soils under recently established J. virginiana forests and adjacent native C₄-dominated grassland to assess changes in potential soil nitrogen (N) transformations and plant available N. Over a 2-year period, concentrations of extractable inorganic N were measured in soils from forest and grassland sites. Potential gross N ammonification, nitrification, and consumption rates were determined using ¹⁵N isotope-dilution under laboratory conditions, controlling for soil temperature and moisture content. Potential nitrification rates (V_max) and microbial biomass, as well as soil physical and chemical properties were also assessed. Extractable NH₄⁺ concentrations were significantly greater in grassland soils across the study period (P ≤ 0.01), but analysis by date indicated that differences in extractable inorganic N occurred more frequently in fall and winter, when grasses were senescent but J. virginiana was still active. Laboratory-based rates of gross N mineralization (ammonification) and nitrification were greater in grassland soils (P ≤ 0.05), but only on one of four dates. Potential nitrification rates (V_max) were an order of magnitude greater than gross nitrification rates in both ecosystems, suggesting that nitrification is highly constrained by NH₄⁺ availability. Differences in plant uptake of N, C inputs, and soil microclimate as forests replace grasslands may influence plant available N in the field, as evidenced by seasonal differences in soil extractable NH₄⁺, and total soil C and N accumulation. However, we found few differences in potential soil N transformations under laboratory conditions, suggesting that this grassland-to-forest conversion caused little change in mineralizable organic N pools or potential microbial activity.     

Quantification of the effects of rotation breaks on soil biological properties and their impact on yield decline in sugarcane

Three contrasting rotation breaks (sown pasture, alternate crops and bare fallow) were established at five sites in Queensland, Australia, on land that had been under sugarcane monoculture for at least 20 years. The breaks were in place for 30-42 months at four sites and for 12 months at the fifth site. The effects of the breaks on selected soil biological properties were assessed following the removal of the breaks and before the area was re-planted with sugarcane. At the four sites with the long-term breaks, microbial biomass increased under the pasture break, declined under the bare fallow break and did not change significantly under the crop break, compared to microbial biomass under continual sugarcane. At these sites, populations of the root lesion nematode (Pratylenchus zeae) declined under all three breaks whereas populations of free-living nematodes increased under the pasture and crop breaks but declined under the bare fallow break. At the site with the 12 month breaks, a forage legume pasture increased microbial biomass, reduced lesion nematodes and together with the crop break increased populations of free-living nematodes. At the four sites with the long-term breaks there was an increase in the ratio of fungal:bacterial fatty acids and an increase in fatty acid 16:1ω5c (used as a biomarker for mycorrhizal fungi) under the pasture and crop breaks. Also at these sites, the soil microbial community under the pasture, crop and bare fallow breaks, respectively, showed increased, no change or diminished capacity to utilize carbon substrates compared to the soil microbial community under continual sugarcane. The yield of the sugarcane crop following all three breaks was significantly higher than the yield of the crop following continual sugarcane at each of the sites with the long-term breaks. Examination of the longevity of the effect of the rotation breaks on soil biological properties at the sites with the long-term breaks, suggested that effects on some soil organisms (e.g. lesion nematodes) maybe short-lived.     

A method for siting parks and reserves with special reference to Columbia and Ecuador

Many tropical countries contain large numbers of species with small geographical ranges, here, for convenience, termed endemics. South America, for example, harbours 440 endemic land birds having ranges of less than 50 000 km². These comprise about a quarter of the terrestrial avifauna of the continent. Such species are exceptionally vulnerable to deforestation and their preservation will require siting future parks or reserves in just the right places. Using Colombia and Ecuador as an illustration, we describe a simple procedure for locating areas of concentrated endemism that would be optimal for future protection. Unfortunately, there is little correspondence between the points of maximal endemism and the locations of existing and projected reserves in the two countries. The advantages and limitations of using satellite photos for habitat evaluation in centres of endemism are also discussed.     

Comparison of interlaboratory variation in amino acid analysis and rat growth assays for evaluating protein quality

Estimates of inter- and intralaboratory variation of protein efficiency ratio (PER), relative PER (RPER), net protein ratio (NPR), relative NPR (RNPR), and nitrogen utilization (NU) were compared with those of amino acid analysis in the same batches of 7 protein sources (ANRC casein, egg white solids, minced beef, soy assay protein, rapeseed protein concentrate, pea flour, and whole wheat flour). Interlaboratory variation (estimated as between-laboratories coefficients of variation, CV) of NPR and RNPR (up to 6.0%) was lower than that of PER (up to 20.2%) and RPER (up to 18.5%). The interlaboratory determination of NPR and RNPR was also more reproducible than that of most essential amino acids (CV up to 10.0%), especially tryptophan (CV up to 23.7%), cystine (CV up to 17.6%), and methionine (CV up to 16.1%). Intralaboratory variation (estimated as within-laboratories CV) of amino acid analysis (up to 4.7%), however, was comparable to that of protein quality indices in most protein sources (up to 6.0%). The significant (P less than 0.01) positive correlations (r = 0.68-0.74) between amino acid scores and protein quality indices based on rat growth were further improved when amino acid scores were corrected for digestibility of protein (r = 0.73-0.78) or individual amino acids (r = 0.79-0.82).     

The structure of the upper atmosphere of mars: In situ accelerometer measurements from mars global surveyor

The Mars Global Surveyor (MGS) z-axis accelerometer has obtained over 200 vertical structures of thermospheric density, temperature, and pressure, ranging from 110 to 170 kilometers, compared to only three previous such vertical structures. In November 1997, a regional dust storm in the Southern Hemisphere triggered an unexpectedly large thermospheric response at mid-northern latitudes, increasing the altitude of thermospheric pressure surfaces there by as much as 8 kilometers and indicating a strong global thermospheric response to a regional dust storm. Throughout the MGS mission, thermospheric density bulges have been detected on opposite sides of the planet near 90 degreesE and 90 degreesW, in the vicinity of maximum terrain heights. This wave 2 pattern may be caused by topographically-forced planetary waves propagating up from the lower atmosphere.