Validation of liquid chromatographic method for assay of calcitriol and alfacalcidol in capsule formulations

The validation of a liquid chromatographic procedure suitable for the determination of calcitriol and alfacalcidol in their respective formulations labeled to contain at least 0.25 micrograms drug per unit is described. The capsule content is diluted and chromatographed in 15-20 min on silica columns (5 micron) with a mobile phase of hexane-tetrahydrofuran-methylene dichloride-isopropanol (72 + 12 + 12 + 4, v/v) with detection at 254 nm. The calibration curve is linear. Recoveries of "spikes" averaged 101% with a standard deviation of 2%. Precision was better than 1.5%.     

Dual infections of red deer (Cervus elaphus) by Leptospira interrogans serovars copenhageni and hardjo

On a property in the Nelson District, blood and urine samples were taken from red deer (Cervus elaphus) from which low (<1:100) antibody titres to serovar copenhageni and suspected leptospiral abortions had previously been reported. A total of 27 hinds were sampled. Microscopic agglutination test (MAT) titres in sera ranging from 1:32 to 1:128 were found in six animals. Thirteen leptospiral isolations were made from nine of the 27 urine samples. Four of these were typed as copenhageni and nine as hardjo. Two cultures were prepared from each urine sample and hardjo and copenhageni were both isolated from single urine samples from two animals. None of the 27 deer had serum MAT titres at 1:32 or above to copenhageni.     

Submitting canine blood for prothrombin time and partial thromboplastin time determinations

Practitioners commonly submit samples from dogs for partial thromboplastin time and prothrombin time determinations. Controversy exists as to the necessity for rapid separation of plasma and cells, and submission of the plasma on ice (or frozen). The purpose of this study was to address three questions. First, is it better to submit plasma or is whole blood satisfactory? Second, is it necessary to refrigerate the sample or is maintenance at room temperature (20° C) adequate? Third, does the sample have to arrive at the laboratory within a few hours of collection or can reliable partial thromboplastin time/prothrombin time determinations be made on samples up to 48 hours old?It has been shown by this study that reliable partial thromboplastin time and prothrombin time determinations can be carried out on canine plasma for up to 48 hours after collection regardless of whether or not the plasma is separated immediately; however the samples must be kept at 4°C. If the samples are maintained at room temperature, reliable prothrombin time determinations can be obtained for up to six hours after collection regardless of whether or not the plasma is separated immediately. Reliable partial thromboplastin time determinations can be made on plasma stored at 20°C for up to 24 hours after collection and possibly longer (up to 48 hours) if the plasma has been separated immediately.     

Caprine beta-Mannosidosis in Kids from an Ontario Herd

Caprine β-mannosidosis, a fatal inherited deficiency of the lysosomal enzyme β-mannosidase, was diagnosed in neonatal female Nubian crossbred twin kids from a small herd near Guelph, Ontario. The kids had been tetraplegic since birth, with whole body tremors, abnormal nystagmus and an intention tremor of the head.